Proliferative Effectin Vitroof Follicle-Stimulating Hormone on the Left Ovary of the Chick Embryo

Velázquez, P.N.; Peralta, Idanely Mora; Pedernera, E.

doi:10.1006/gcen.1996.6796

Cited by 16 publications

(2 citation statements)

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“…In vitro dispersed cell cultures of left embryonic chicken ovary, treated with FSH, increased cell proliferation in later stages of incubation and estrogen and testosterone synthesis in all stages of incubation studied (Pedernera et al, 1999). This proliferation is observed in poorly differentiated epithelial cells from the medulla and pregranulosa cells from the cortex (Velaquez et al, 1997). The above inductions of From the above observations it is evident that as the chicken medulla transforms itself into a lacunar system, this tissue possesses endocrine responsiveness and the ability to modulate ovarian development.…”

Section: Discussionmentioning

confidence: 93%

Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

Moore

Uribe-Aranzábal

Boggs

et al. 2007

Journal of Morphology

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American alligator (Alligator mississippiensis) ovary development is incomplete at hatching. During the months following hatching, the cortical processes of oogenesis started in ovo continues and folliculogenesis is initiated. Additionally, the medullary region of the gonad undergoes dramatic restructuring. We describe alligator ovarian histology at hatching, 1 week, 1 month, and 3 months of age in order to characterize the timing of morphological development and compare these findings to chicken ovary development. At hatching, the ovarian cortex presents a germinal epithelium containing oogonia and a few primary oocytes irregularly scattered between somatic epithelial cells. The hatchling medulla shows fragmentation indicative of the formation of lacunae. By 1 week of age, oocytes form growing nests and show increased interactions with somatic cells, indicative of the initiation of folliculogenesis. Medullary lacunae increase in diameter and contain secretory material in their lumen. At 1 month, nest sizes and lacunar diameters continue to enlarge. Pachytene oocytes surrounded by somatic cells are more frequent. Trabeculae composed of dense irregular connective tissue divide cortical nests. Three months after hatching oocytes in meiotic stages of prophase I up to diplotene are present. The ovary displays many enlarged follicles with oocytes in diplotene arrest, thecal layers, lampbrush chromosomes, and complete layers of follicular cells. The medulla is an elaborated complex of vascularized lacunae underlying the cortex and often containing discrete lymphoid aggregates. While the general morphology of the alligator ovary is similar to that of the chicken ovary, the progression of oogenesis and folliculogenesis around hatching is notably slower in alligators. Diplotene oocytes are observed at hatching in chickens, but not until 3 months in alligators. Folliculogenesis is completed at 3 weeks in chickens whereas it is still progressing at 3 months in alligators.

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Section: Discussionmentioning

confidence: 93%

Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

Moore

Uribe-Aranzábal

Boggs

et al. 2007

Journal of Morphology

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“…FSH stimulates cell proliferation, growth, and DNA synthesis, enhances the secretion of steroids in the embryonic ovary, provokes structural changes of the ovary, and increases the cell numbers of different ovarian cell subpopulations of hatched chicks (Velázquez et al, 1997;Méndez-Herrera et al, 1998;Ohtani et al, 2001). FSH-binding cells have been detected on Day 6.5 of incubation in the left embryonic ovarian cells.…”

Section: Introductionmentioning

confidence: 97%

Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation

Liu

Zeng

Cao

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathways. Ovarian cells were treated with FSH alone or in the presence of forskolin (FRSK), PKA inhibitor (H 89 ), PKC activator (PMA) or inhibitor (H 7 ). The germ cell number was counted from micropictures. The immunocytochemistry of proliferating cell nuclear antigen (PCNA) was applied to identify the proliferating cells. The germ cell labeling index (LI) was determined for cell proliferation. The FSH treatment increased the germ cell number, and this stimulating effect was enhanced by FRSK or PMA, but inhibited by H 89 or H 7 in a dose-dependent manner. Moreover, the PCNA-LI showed parallel changes with germ cell numbers. This study suggests that FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways.

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Adenohypophysis regulates cell proliferation in the gonads of the developing chick embryo

2005

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The aim of the present study was to evaluate the effect of hypophysectomy on cell proliferation in the left ovary and the left testis of 8- to 14-day-old chick embryos. Hypophysectomy was performed by the partial decapitation technique. At 44-46 h of incubation, chick embryo heads were sectioned at the mesencephalic level and the prosencephalic region removed. Embryos were further incubated until 8-14 days of development. Cell division was evaluated by bromodeoxyuridine (BrdU) incorporation and by counting the total number of somatic and germ cells in the gonads. The ovary displayed an exponential increase in the number of somatic and germ cells and a higher rate of BrdU incorporation compared to the testis. BrdU incorporation was reduced in the ovary of hypophysectomized embryos at 9-14 days of incubation, while in the testis, the reduction was significant at 14 days of development. Changes in the total number of somatic and germ cells further suggest that the absence of hypophysis affects the growth of the ovary earlier than the growth of the testis. Reduction in the number of somatic and germ cells after hypophysectomy in the ovary was reversed by a hypophyseal graft on the chorioallantoic membrane. The adenohypophysis regulates, probably through gonadotropic hormones, proliferation of somatic and germ cells in the gonads during chick embryo development.

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Proliferative Effectin Vitroof Follicle-Stimulating Hormone on the Left Ovary of the Chick Embryo

Cited by 16 publications

References 0 publications

Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation

Adenohypophysis regulates cell proliferation in the gonads of the developing chick embryo

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