Proliferative Enteritis Associated With Lawsonia Intracellularis in a Japanese Macaque (Macaca Fuscata)

Lafortune, M; Wellehan, James F. X.; Jacobson, Elliott R.; Troutman, J. Mitchell; Gebhart, Connie J.; Thompson, Margaret Susan

doi:10.1638/03-080

Cited by 15 publications

(13 citation statements)

References 7 publications

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“…as well as H. cinaedi and recently identified H. macacae have been isolated from inflamed colons of rhesus monkeys (Fox et al, 2001a,b;Fox et al, 2007). Similar lesions have been identified in Japanese macaques (M. fuscata) (Wamsley et al, 2005;Lafortune et al, 2004). Persistent infection of rhesus macaques with H. macacae associated with colonic adenocarcinoma has been described (Marini et al, 2010;Lertpiriyapong et al, 2014).…”

Section: F Helicobacter Sppmentioning

confidence: 79%

Nonhuman Primates

Magden

Mansfield²,

Simmons

et al. 2015

Laboratory Animal Medicine

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Section: F Helicobacter Sppmentioning

confidence: 79%

Nonhuman Primates

Magden

Mansfield²,

Simmons

et al. 2015

Laboratory Animal Medicine

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“…In horses, proliferative enteropathy is considered an emerging disease [5]. In non-human primates, there are reports of outbreaks of the hemorrhagic form of proliferative enteropathy, causing sudden death in affected animals [6,7].…”

Section: Introductionmentioning

confidence: 99%

Survival of Lawsonia intracellularis in porcine peripheral blood monocyte-derived macrophages

et al. 2020

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Lawsonia intracellularis, an obligately intracellular enteric bacterium, infects intestinal epithelial cells, but may also be found within macrophages in the intestinal lamina propria of affected pigs. Macrophages play an important role in host defense against infectious agents, but the role of this cell in L. intracellularis infection is not well understood. The aim of this study was to evaluate the permissibility of macrophages to L. intracellularis infection in vitro. Pure culture of L. intracellularis was added to swine peripheral blood monocyte-derived macrophages. Viability of intracytoplasmic L. intracellularis was evaluated at different time points by transmission electron microscopy (TEM). Potential replication of L. intracellularis in macrophages was also evaluated by qPCR. By TEM, phagocytosis L. intracellularis within of phagolysosomes were observed 1-hour post-infection (hpi) and bacterial structures in binary fission at 48 hpi. The number of intracellular bacteria was determined at 1, 4, 24, 48, and 72 hpi by qPCR in infected macrophages and compared to the number of intracellular bacteria from culture in McCoy cells. In both cell lines, the amount of L. intracellularis was decreased at 4 hpiand increased at 24 hpi. The number of intracellular bacteria continued to increase in McCoy cells over time. This is the first study showing interaction, survival and propagation of L. intracellularis in macrophages. These findings are critical to establish an experimental model for future studies of the pathogenesis of porcine proliferative enteropathy and the potential persistence of L. intracellularis in macrophages during chronic infections.

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“…Most concerned species are pigs, horses and hamsters 3. Non‐human primates are also susceptible, and cases have been described in Japanese macaques ( Macaca fuscata ) and rhesus macaques ( M mulatta ) 4 5 . L intracellularis has never been identified as an enteric pathogen in humans and is not considered to be a zoonotic disease 2.…”

Section: Introductionmentioning

confidence: 99%

Suspicion of epizootic Lawsonia intracellularis disease in a group of pileated gibbons (Hylobates pileatus)

Quintard

François-Brazier²,

Giorgiadis³

et al. 2020

Vet Record Case Reports

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Within a month, all five individuals of a pileated gibbon (Hylobates pileatus) family presented the same symptoms: diarrhoea, vomiting and severe apathy. During this first outbreak, three of them died acutely and a fourth died during another outbreak 7 months later. Extensive virology, bacteriology and parasitology undertaken on faeces did not reveal any pathogen. Haematology and extensive biochemistry did not demonstrate any obvious medical disorder. Necropsy revealed a severe proliferative enteropathy, and rare argyrophilic bacteria were noted within the lumen of hyperplastic colonic glands on histology with silver staining. Specific faecal PCR was positive for Lawsonia intracellularis infection. These combined elements led to a strong suspicion of L intracellularis epizooty, despite the absence of large numbers of argyrophilic bacteria, as usually expected in this disease process. L intracellularis has mostly been described in pigs even if reported in a broad range of mammalian species including primates. L intracellularis may be underdiagnosed in zoos as it requires a faecal PCR for identification and silver staining for histological visualisation.

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Proliferative Enteritis Associated With Lawsonia Intracellularis in a Japanese Macaque (Macaca Fuscata)

Cited by 15 publications

References 7 publications

Nonhuman Primates

Nonhuman Primates

Survival of Lawsonia intracellularis in porcine peripheral blood monocyte-derived macrophages

Suspicion of epizootic Lawsonia intracellularis disease in a group of pileated gibbons (Hylobates pileatus)

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