Proliferative response and metabolic effects of growth factors in human hepatocytes

Functional Assessment of the Quality of Human Hepatocyte Preparations for Cell Transplantation

“…In addition Banked hepatocytes can be used on demand for programmed infusions and are available for immediate use to intrinsic donor characteristics, however, other factors related to the procurement of liver sample and hepato-in urgent cases. Different cryopreservation protocols have been proposed, but a universal consensus about the cyte isolation procedure can contribute to the observed variability in drug-metabolizing activities (11,15). Drug-most appropriate freezing procedure has not been reached (1,2,17,20,42).…”

“…Mild steatosis Freshly isolated hepatocytes were sedimented and suspended at a density of 10 × 10 6 viable cells/ml of (<10% steatotic hepatocytes) has been reported to not affect the outcome of hepatocyte isolation, but severe cyopreservation medium (90% University of Wisconsin solution and 10% DMSO, v/v maintained at 4°C). Then steatosis decreases hepatocyte viability and yield, and also the levels of drug metabolising cytochrome P450 2 ml of the resultant cell suspension was aliquoted into cryovials (20 × 10 6 cells/vial), which were transferred (P450) enzymes (2,3,10,13,15). Short warm ischemia times (<30 min) and flushing the tissue with preserva-into isopropanol containers (Nalgene; Nalge Nunc International Rochester, NY) and stored at −80°C (freezing tion solutions are recommended (7,11,38).…”