Proliferative responses of rat ventral prostate: Effects of variations in organ culture media and methodology

Buchanan, Lynn; Riches, Andrew

doi:10.1002/pros.2990080108

Cited by 8 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Insulin can act directly through its own high-affinity receptor [7,109] or through low-affinity binding to the insulin-like growth factor I (IGF-I) receptor [24,90,91,109]. Insulin and IGF-I receptors have been reported for normal and neoplastic rat and human prostates [8,13,17,34,47,87,92], and insulin and IGF-I are mitogenic for prostatic epithelial cells in vitro [8,12,17,19,47,71,87]. Moreover, insulin has been demonstrated to be an essential component of serum-free media and, in the case of the neonatal mouse SV, is required to obtain maximal response to testosterone [116].…”

Section: Insulin and Insulin-like Growth Factorsmentioning

confidence: 99%

Growth factors as mediators of androgen action during the development of the male urogenital tract

et al. 1995

View full text Add to dashboard Cite

Studies on the developing prostate and SV suggest that androgens act via mesenchymal AR to elicit synthesis and secretion of various autocrine and paracrine factors that regulate epithelial and stromal growth and differentiation. Clearly, the global regulation of epithelial growth and ductal branching morphogenesis is a complex multifactorial process involving the interplay of many diffusible factors (both positive and negative regulators), extracellular matrix molecules, cell-surface receptors for growth factors, receptors for extracellular matrix molecules, and matrix-degrading enzymes. Future progress will certainly be dependent upon the utilization of appropriate, biologically relevant models to examine the respective roles of various growth factors in the growth and development of androgen target organs.

show abstract

Section: Insulin and Insulin-like Growth Factorsmentioning

confidence: 99%

Growth factors as mediators of androgen action during the development of the male urogenital tract

et al. 1995

View full text Add to dashboard Cite

show abstract

“…In vitro procedures, both organ and cell cultures, have been particularly useful for investigating the role of androgens in the development, growth, and function of the male reproductive tract, because the complex hormonal interactions that occur in vivo can be eliminated or controlled (10)(11)(12)(13)(14)(15)(16). One limitation of past studies has been the suboptimal growth and morphogenesis of male accessory gland rudiments in organ culture compared to in vivo development.…”

mentioning

confidence: 99%

Postnatal Growth of Mouse Seminal Vesicle Is Dependent on 5α-Dihydrotestosterone*

Shima¹,

Tsuji²,

Young³

et al. 1990

Endocrinology

View full text Add to dashboard Cite

The seminal vesicles (SV) develop from the lower portion of the Wolffian ducts (WD) in response to androgens, which prevent their degeneration and subsequently stimulate organogenesis of the epididymis, vas deferens, seminal vesicles, and ejaculatory ducts. Earlier studies suggest that testosterone (T) is the active androgen for WD development. By contrast, development of urogenital sinus and external genitalia is dependent upon 5 alpha-dihydrotestosterone (DHT), produced by 5 alpha-reductase within the target tissue itself. To reevaluate the possible role of DHT during SV morphogenesis, SVs from 0-day-old (day of birth) mice were grown for 3, 6, or 9 days in either serum-free or serum-containing medium in the presence or absence of T (10(-7) M) or DHT (10(-8) M). The serum-free medium consisted of Ham's F-12-Dulbecco's Modified Eagle's Medium (1:1) containing insulin, transferrin, cholera toxin, BSA, and epidermal growth factor. The serum-containing medium was Ham's F-12-Dulbecco's Modified Eagle's Medium containing 10% fetal calf serum. Epithelial branching morphogenesis of SVs occurred in serum-free or serum-containing medium supplemented with either T or DHT and was comparable to that of SVs of similar ages in vivo. In serum-containing medium the DNA content of the cultures was about 2-fold higher in T-containing vs. T-deficient medium. However, in serum-free medium the DNA content was the same in cultures grown with or without T. SVs cultured under serum-free conditions in the presence of T plus 390 MSD (17 beta-N,N-diisopropylcarbamoyl-4-aza-5 alpha-androstan-3-one, an inhibitor of 5 alpha-reductase) were completely inhibited in their development, while in the presence of DHT plus 390 MSD, branching morphogenesis was comparable to that in SVs cultured in the presence of T or DHT alone. In medium lacking either T or DHT, SV development was inhibited. In addition, it was confirmed by TLC that [1 beta,2 beta-3H]T was converted into [3H]DHT at the ratio of 15.4% for the first 2 days and at 35.3% for the subsequent 2 days of the culture of SVs in serum-free medium. These data demonstrate that T is important as a precursor of DHT and DHT is the major androgen in the postnatal development of mouse SVs from the lower WD.

show abstract

“…Organ culture Ventral prostate from young adult Wistar rats (4-6 months old) was maintained in serum free and serum-supplemented (5, 10 or 20% foetal calf serum) organ culture for 4 days (Riches et al, 1976;Mistry et al, 1982;Buchanan & Riches, 1985, 1986 …”

Section: Methodsmentioning

confidence: 99%

“…Thus, in the present study, an in vitro model of induced DNA synthesis in cultured rat ventral prostate (Mistry et al, 1982;Buchanan & Riches, 1985, 1986 …”

mentioning

confidence: 87%

Testosterone-induced DNA synthesis in cultured rat ventral prostate: effects of estracyt and its derivatives

Buchanan¹,

Ac²

1987

Br J Cancer

View full text Add to dashboard Cite

Summary Testosterone-induced DNA synthesis in cultured rat ventral prostate was used to compare the direct effects of EstracytR and EmcytR with that of their metabolite, estramustine, and their carrier-hormone, oestradiol-17/ on prostatic growth. In serum-supplemented medium (5-20% FCS), all the compounds were equally effective in suppressing testosterone stimulated DNA synthesis which was reduced by between 40-50%, whereas in serum-free medium the estramustine compounds were consistently less effective than oestradiol-17,B. In the presence of 4 x 10 -9M testosterone in serum-free medium, stimulated DNA synthesis was reduced by 15-30% following incubation with 4 x 10-7 M of EstracytR, EmcytR and estramustine and by 60% with 4 x 10-7 M oestradiol-17f3. Thus, none of the estramustine compounds appear to offer any selective advantage over that of oestradiol-17/1 in suppressing prostatic DNA synthesis at the target tissue level.Prostatic cancer is the most common malignancy of the male urogenital tract and a leading cause of death due to cancer in aging men (Chisholm, 1981;Flanders, 1984 (Tew, 1983;Hoisaeter, 1984).In both the rat (Plym-Forshell & Nilsson, 1974;Hoisaeter, 1976aHoisaeter, , 1977 Hoisaeter & Bakke, 1983) and man (PlymForshell et al., 1976;Sandberg, 1983), Estracyt is rapidly dephosphorylated to yield estramustine and its dehydrogenated counterpart, estromustine. These metabolites are selectively retained in the prostate by interactions with estramustine binding protein (Forsgren et al., 1979(Forsgren et al., , 1981Bjork et al., 1982) and subsequent intracellular hydrolysis of these compounds slowly liberates free nitrogen mustard and oestrogen moieties. However, the question remains whether the antiprostatic actions of Estracyt are mediated by the oestrogen moiety, the nitrogen mustard moiety or the intact drug complex itself.While in vivo studies have shown that Estracyt is a more potent inhibitor of rat prostatic DNA synthesis than either its hormone or cytostatic parts (Hoisaeter, 1976b(Hoisaeter, , 1977, such comparisons are limited by the extensive metabolism of Estracyt in vivo. Thus, in the present study, an in vitro model of induced DNA synthesis in cultured rat ventral prostate (Mistry et al., 1982;Buchanan & Riches, 1985, 1986

show abstract

Proliferative responses of rat ventral prostate: Effects of variations in organ culture media and methodology

Cited by 8 publications

References 15 publications

Growth factors as mediators of androgen action during the development of the male urogenital tract

Growth factors as mediators of androgen action during the development of the male urogenital tract

Postnatal Growth of Mouse Seminal Vesicle Is Dependent on 5α-Dihydrotestosterone*

Testosterone-induced DNA synthesis in cultured rat ventral prostate: effects of estracyt and its derivatives

Contact Info

Product

Resources

About