Proline accumulation and glutamine synthetase activity are increased by salt-induced proteolysis in cashew leaves

Silveira, Joaquim Albenísio Gomes da; Viégas, Ricardo de Almeida; Rocha, Iza M. A.; Monteiro-Moreira, Ana Cristina de Oliveira; Moreira, Renato de Azevedo; Oliveira, José T.A.

doi:10.1078/0176-1617-00890

Cited by 202 publications

(116 citation statements)

References 27 publications

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“…The salinity-stress tolerance mechanism is known as the ability of plant cells to osmotically adjust and to accumulate organic solutes such as sugars and amino acids (Silveira et al, 2003). Our study showed that moderate salinity levels (EC 1.76 dS.m -1 ) did not affect the amino acid, proline and carbohydrate; they were only affected by higher salt concentrations.…”

Section: Morphologicalmentioning

confidence: 54%

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

Cavalcante¹,

Santos²,

Filho³

et al. 2018

Aust J Crop Sci

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The aim of the current study is to assess the growth response, biochemical changes, photosynthetic pigments content and gas exchanges of physic nut (Jatropha curcas) grown under natural saline conditions. The experiment was arranged in a completely randomized design based on the following soil electrical conductivities: 0.29 dS.m -1 (control), 1.76 dS.m -1 (moderate salt concentration), 2.61 dS.m -1 (high salt concentration), 3.79 dS.m -1 (very high salt concentration). Physic nut plants were kept under saline conditions for 19 days in greenhouse. Plant growth analyses were performed on a weekly basis. Plant biomass allocation was quantified at the end of the experiment. Leaf gas exchange, stomatal conductance, Fv/Fm and quantum yield were quantified 102 days after planting. Photosynthetic pigments, amino acids, proline and carbohydrates in fresh leaf tissue were also quantified. The leaf antioxidant enzymes catalase (CAT) and ascorbate peroxidase (APX) were quantified. Although there was no alteration in biomass allocation, the initial growth of J. curcas was gradually reduced by increasing salt concentration, which was observed through reduced plant height, stem diameter, and total number of leaves. Soil electrical conductivity 3.79 dS.m -1 was lethal to seedlings. Seedlings exposed to salt stress had their photosynthesis, stomatal conductance, transpiration and effective photochemical efficiency reduced, and their catalase and ascorbate peroxidase enzyme activity increased. Amino acids, proline and carbohydrate concentrations increased due to salt stress, whereas there was decrease in the chlorophyll content. J. curcas is sensitive to soil salinity at electrical conductivity levels higher than 1.76 dS.m -1 . To some extent, salinity effects can be relieved by osmolyte accumulation and by greater antioxidant activity; however, these factors were not sufficient to keep plant growth within normal rates.

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Section: Morphologicalmentioning

confidence: 54%

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

Cavalcante¹,

Santos²,

Filho³

et al. 2018

Aust J Crop Sci

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“…Reactive Oxygen species (ROS), mainly hydrogen peroxide (H 2 O 2 ) and the superoxide radical (O 2 − ), may react with several molecules generated by cells causing oxidative damage which can interfere with the regular development of the cells (Marino et al, 2009;Silveira et al, 2003). The plants protect their cells and sub-cell invasive geophyte species.…”

Section: Resultsmentioning

confidence: 99%

The presence of Microlobius foetidus cause changes in the antioxidant defense of Urochloa decumbens?

et al. 2015

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Urochloa decumbens (Stapf) R. D. Webster (Poaceae) is an exotic species with has spread rapidly through the Cerrado area of Pantanal, Mato Grosso do Sul, Brazil. It has covered the soil aggressively turning it into cultivated pastures. Thus, it has become a challenge to protect native areas due its capacity of exclusion of native species. It has been observed that Microlobius foetidus (Jacq.) M.Sousa & G.Andrade species (Fabaceae) shows a dominant pattern over the development of U. decumbens. This work shows that M. foetidus interfere on the natural growth of U. decumbens within 10 m ratio. Between 15 and 20 m, it was observed an increase of Importance Value index (IVI) and Relative cover (RC) values. It was also observed a variation on the antioxidant defense system of U. decumbens within 10m ratio from M. foetidus. The enzymes superoxide dismutase, catalase and peroxidase present higher levels of activity then those found for glutathione reductase. This data indicates that M. foetidus may have an effect on U. decumbens, increase the activity of antioxidant enzymes. This effect probably happens as means to neutralize the toxic effects of the oxygen generated due to the presence of allelochemicals, which increases oxidative stress.Keywords: antioxidant enzymes, signal grass, garlic stick, competition. A presença de

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“…Letras distintas, acima das barras, indicam médias significativamente diferentes pelo teste de Tukey (p<0,05); ns: diferenças não-significativas pela análise de variância (p>0,05). celular ou se é meramente um sintoma de injúria (Silveira et al, 2003). De acordo com Errabi et al (2006), o aumento induzido por estresse hídrico é fracamente envolvido com ajustamento osmótico na célula.…”

Section: Resultsunclassified

“…Em plantas superiores, o acúmulo de prolina pode estar relacionado à síntese "de novo" e à diminuição de seu catabolismo. Porém, a baixa utilização de prolina durante o estresse, em conseqüência da redução na síntese protéica e do aumento na proteólise, também pode contribuir para seu acúmulo (Hare et al, 1998;Silveira et al, 2003). No entanto, Hien et al (2003) enfatizaram que toda a controvérsia em torno do papel da prolina pode refletir a predominância de sua ação em outros mecanismos de tolerância, que não o ajustamento osmótico.…”

Section: Introductionunclassified

Alterações no perfil de frações nitrogenadas em calos de cana-de-açúcar induzidas por deficit hídrico

Brito

Silveira

Lima

et al. 2008

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o uso de marcadores bioquímicos e fisiológicos, na caracterização do estresse hídrico em calos de cultivares de cana-de-açúcar (Sacharum sp.), RB 72 454 (sensível) e SP 813250 (resistente), contrastantes quanto à resistência à seca em campo. O delineamento experimental foi o inteiramente casualizado, em esquema fatorial de 2x5 [cultivar x doses de polietilenoglicol (PEG)], com dez tratamentos e três repetições. Os calos foram submetidos a concentrações de PEG correspondentes aos potenciais osmóticos de 0, -0,3, -0,6, -0,9 e -1,2 MPa, por 120 horas. A variação no conteúdo relativo de água e na umidade não foi significativamente diferente entre as cultivares. Entretanto, foi observada a tendência de aumento no vazamento de eletrólitos, em conseqüência da diminuição do potencial osmótico na cultivar tolerante. Na cultivar sensível, observou-se tendência de aumento de prolina e, na resistente, diminuição, embora os níveis não tenham sido afetados pelo deficit hídrico. As concentrações de aminoácidos livres foram maiores na sensível. Houve queda nas concentrações de amônia, em ambas cultivares. Os níveis de proteínas não foram afetados pelo PEG. O perfil protéico por SDS-PAGE não mostrou aumento induzido por PEG, na intensidade das bandas correspondentes aos peptídeos entre 14 e 66 kDa. Os marcadores bioquímicos e fisiológicos não foram relacionados ao grau diferencial de resistência observado nas cultivares em condições de campo.Termos para indexação: Saccharum sp., ajustamento osmótico, cultivo de calos, solutos orgânicos. Alterations on the profile of nitrogenous fractions in sugarcane calluses induced by water deficitAbstract -The aim of this work was to evaluate the use of physiological and biochemical parameters, in the characterization of water stress in sugarcane (Sacharum sp.) calluses, cultivars RB 72454 (sensible) and SP 813250 (resistant), differing in drought resistance in the field. The experimental design was completely randomized with a factorial scheme 2x5 [cultivar x concentrations of polyethylene glycol (PEG)], with ten treatments and three replicates. Calluses were submitted to PEG concentrations corresponding to osmotic potentials of 0, -0.3, -0.6, -0.9 and -1.2 MPa, during 120 hours. The variation of relative water content and humidity showed no significant differences between the cultivars. However, it was observed a trend of increase in electrolyte leakage, in the resistant cultivar related to diminishig of the osmotic potential in resistant cultivar. In the sensible cultivar, it was observed a trend of increase in proline, while the resistant one showed decrease, though proline levels were not affected by the water deficit. The amino acid concentrations were bigger in the sensible cultivar. There was a decrease of ammonia in both cultivars. Protein levels were not affected by the PEG. The protein profile evaluated by SDS-PAGE showed no PEG-induced increase in the intensity of bands corresponding to peptides between 14 and 66 kDa. The physiological...

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Proline accumulation and glutamine synthetase activity are increased by salt-induced proteolysis in cashew leaves

Cited by 202 publications

References 27 publications

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

The presence of Microlobius foetidus cause changes in the antioxidant defense of Urochloa decumbens?

Alterações no perfil de frações nitrogenadas em calos de cana-de-açúcar induzidas por deficit hídrico

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