Proline accumulation induces the production of total phenolics in transgenic tobacco plants under water deficit without increasing the G6PDH activity

Silva, Fláive Loyze Baldassarini; Vieira, Luiz Gonzaga Esteves; Ribas, Alessandra Ferreira; Moro, Adriana Lima; Neris, Daniel Moreira; Pacheco, Ana Cláudia

doi:10.1007/s40626-018-0119-0

Cited by 51 publications

(21 citation statements)

References 51 publications

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“…In our study, we also found the elevated levels of free proline in all transgenic sweet potato plant lines compared with the untransformed plants under water deficit stress, suggesting that XvSap1 could induce synthesis of proline genes to confer drought tolerance in sweet potato. Our findings are consistent with the results obtained from overexpressing TsVP and BetA in maize (Wei et al, 2011), G6PDH in tobacco plants (Silva et al, 2018), and BrCIPK1 in rice (Abdula et al, 2016). The biochemical relation between the action of the XvSap1 gene and the high proline concentration in the transgenic sweet potato plants seems to be a sensitive balance.…”

Section: Discussionsupporting

confidence: 92%

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

2019

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Drought stress often leads to reduced yields and is a perilous delimiter for expanded cultivation and increased productivity of sweet potato. Cell wall stabilization proteins have been identified to play a pivotal role in mechanical stabilization during desiccation stress mitigation in plants. They are involved in numerous cellular processes that modify cell wall properties to tolerate the mechanical stress during dehydration. This provides a plausible approach to engineer crops for enhanced stable yields under adverse climatic conditions. In this study, we genetically engineered sweet potato cv. Jewel with XvSap1 gene encoding a protein related to cell wall stabilization, isolated from the resurrection plant Xerophyta viscosa, under stress-inducible XvPSap1 promoter via Agrobacterium-mediated transformation. Detection of the transgene by PCR, Southern blot, and quantitative real-time PCR (qRT-PCR) analyses revealed the integration of XvSap1 in the three independent events. Phenotypic evaluation of shoot length, number of leaves, and yield revealed that the transgenic plants grew better than the wild-type plants under drought stress. Assessment of biochemical indices during drought stress showed higher levels of chlorophyll, free proline, and relative water content and decreased lipid peroxidation in transgenic plants than in wild types. Our findings demonstrate that XvSap1 enhances drought tolerance in transgenic sweet potato without causing deleterious phenotypic and yield changes. The transgenic drought-tolerant sweet potato lines provide a valuable resource as a drought-tolerant crop on arid lands of the world.

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Section: Discussionsupporting

confidence: 92%

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

2019

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“…Secondary metabolites are produced in plants to avoid deleterious effect of biotic and abiotic stresses at the expense of plant growth and development (Yang et al, 2018). In plants, flavonoids (Tohge et al, 2018) and proline (Silva et al, 2018) are the two important group of secondary metabolites which are induced in plant under stress condition. We determined total flavonoid content and proline in LE and RE of maize through spectrophotometry technique (Zhou et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…In such condition, plant increases the concentration of secondary metabolites in its leaf and roots which combat any stressing condition (Yang et al, 2018). The commonly determined secondary metabolites in the plants are proline (Silva et al, 2018), flavonoid contents (Tohge et al, 2018), phenyl propanoids (PPs) (Hiruma, 2019), and glucosinolates (GLs) (Czerniawski and Bednarek, 2018). Phyto-hormonically in host, HR inducing microbes supress the E3 ligase polyubiquitination to inhibit GA 3 from degrading DELLA protein (Li et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Yousaf

Hussain

Hamayun

et al. 2019

Preprint

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16Besides acting as growth inducing molecule, Gibberellin (GA 3 ) also confers the compatibility 17 of microbial interactions with host. We inoculated 11 days old Z. mays seedlings grown under 18 hydroponic conditions and high GA 3 levels with Bipolaris sorokiniana (BIPOL) at the spore 19 density (SD) of OD 0.6 . The high level of GA 3 negatively affected the growth of the seedlings, 20 accompanied by the high level of stress deducing secondary metabolites (proline, total 21 flavanoids, phenylpropanoids, and glucosinolides). Moreover, high level of GA 3 produced a 22 hypersensitive response (HR) in the seedlings. The HR developed cross talks with IAA and 23 trans-zeatins and triggered higher production of hypersensitive inducing biomolecules. The 24 other HR co-related biological processes were demonstrated by high phytoalexins level and 25 high protease activities. Such activities ultimately inhibited the colonization of BIPOL on the 26 roots of maize seedlings. The products of the genes expressed at high GA 3 also conferred the 27 deterrence of BIPOL colonization at SD = OD 0.6 . Intriguingly, when we inhibited GA 3 28 biosynthesis in the seedlings with aerially sprayed uniconizole, prior to BIPOL treatment, the 29 BIPOL colonized and subsequently promoted the seedling growth. This low level of GA 3 30 after BIPOL treatment checked the high level of secondary metabolites and hypersensitivity 31 inducing molecules. The results, thus suggested that the aforementioned processes only 32 happened in the BIPOL at SD (OD 0.6 ), whereas the SD at lower levels (OD 0.2 or OD 0.4 ) 33 neither promoted the growth of uniconizole pre-treated seedlings nor produced HR in control 34 seedlings of maize plant.35

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“…A great number of researches have shown that G6PDHs play a critical role in plant growth and abiotic stress responses (Esposito, 2016;Hýsková et al, 2017). Although G6PDH genes have been cloned from several model organisms such tobacco (Scharte et al, 2009;Silva et al, 2018), Arabidopsis (Wakao and Benning, 2005), barley (Cardi et al, 2013;Caretto et al, 2015), wheat (Nemoto and Sasakuma, 2000) and tomato (Landi et al, 2016), there is scarce information about their biological functions in soybean. In this study, we identified nine G6PDH gene family members from the soybean genome, named as GmG6PDH1-9 (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of the Glucose-6-Phosphate Dehydrogenase Family in Soybean and Functional Identification of GmG6PDH2 Involvement in Salt Stress

et al. 2020

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Glucose-6-phosphate dehydrogenase (G6PDH) is known as a critical enzyme responsible for nicotinamide adenine dinucleotide phosphate (NADPH) generation in the pentose phosphate pathway (PPP), and has an essential function in modulating redox homeostasis and stress responsiveness. In the present work, we characterized the nine members of the G6PDH gene family in soybean. Phylogenic analysis and transit peptide prediction showed that these soybean G6PDHs are divided into plastidic (P) and cytosolic (Cy) isoforms. The subcellular locations of five GmG6PDHs were further verified by confocal microscopy in Arabidopsis mesophyll protoplasts. The respective GmG6PDH genes had distinct expression patterns in various soybean tissues and at different times during seed development. Among them, the Cy-G6PDHs were strongly expressed in roots, developing seeds and nodules, while the transcripts of P-G6PDHs were mainly detected in green tissues. In addition, the activities and transcripts of GmG6PDHs were dramatically stimulated by different stress treatments, including salt, osmotic and alkali. Notably, the expression levels of a cytosolic isoform (GmG6PDH2) were extraordinarily high under salt stress and correlated well with the G6PDH enzyme activities, possibly implying a crucial factor for soybean responses to salinity. Enzymatic assay of recombinant GmG6PDH2 proteins expressed in Escherichia coli showed that the enzyme encoded by GmG6PDH2 had functional NADP +-dependent G6PDH activity. Further analysis indicated overexpression of GmG6PDH2 gene could significantly enhance the resistance of transgenic soybean to salt stress by coordinating with the redox states of ascorbic acid and glutathione pool to suppress reactive oxygen species generation. Together, these results indicate that GmG6PDH2 might be the major isoform for NADPH production in PPP, which is involved in the modulation of cellular AsA-GSH cycle to prevent the oxidative damage induced by high salinity.

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Proline accumulation induces the production of total phenolics in transgenic tobacco plants under water deficit without increasing the G6PDH activity

Cited by 51 publications

References 51 publications

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Genome-Wide Analysis of the Glucose-6-Phosphate Dehydrogenase Family in Soybean and Functional Identification of GmG6PDH2 Involvement in Salt Stress

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