Proline and Proline Metabolising Enzymes in in-vitro Selected NaCl-tolerant Brassica juncea L. under Salt Stress

Madan, Shashi

doi:10.1006/anbo.1995.1077

Cited by 125 publications

(62 citation statements)

References 26 publications

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“…It is well known that proline is a non-protein amino acid, and is believed to protect plant tissues against stress by acting as an N-storage compound, osmosolute, and hydrophobic protectant for enzymes and cellular structures (Madan et al 1995). In the present case, the Pro of both AMC and NAMC leaves were comparatively low under stress-free.…”

Section: Resultsmentioning

confidence: 47%

The enhancement of drought tolerance for pigeon pea inoculated by arbuscular mycorrhizae fungi

Qiao¹,

Wen²,

Yu³

et al. 2011

PLANT SOIL ENVIRON

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Pigeon pea (Cajanus cajan) has been rapidly grown in the drought-striken Karst regions of southwest China. Present research aimed to investigate the effects of arbuscular mycorrhizae (AM) on the drought tolerance of pigeon pea, as well as to elucidate the physiological responses of AM-colonized seedlings to the water deficit. As subjected to drought stress, AM symbiosis (AMD) highly led to the positive effects on root system, plant height and stem diameter. AMD demonstrated a remarkably higher chlorophyll content, photosynthetic rate and stomatal conductance. The soluble sugar in AMD was significantly higher than that of the non-AM seedlings (NAMD), indicating the enhanced tolerance at least partially correlated with osmotic solute. Conversely, the proline (Pro) of AMD was lower, revealing the excessive Pro was not imperative for drought tolerance. After 30 days drought stress, AMD gave around a third less lipid peroxides than that of NAMD. Rather, the root activities of AMD were significantly higher than that of the latter after 10 days drought stress. Thereby, AM fungi might substantially elevate the tolerance to drought of pigeon pea, and the cumulative effects contributed to the enhanced tolerance. To date, this has been the first report concerning the enhancement of drought tolerance via AM colonization in this legume species.

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Section: Resultsmentioning

confidence: 47%

The enhancement of drought tolerance for pigeon pea inoculated by arbuscular mycorrhizae fungi

Qiao¹,

Wen²,

Yu³

et al. 2011

PLANT SOIL ENVIRON

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“…The reaction was stared by the addition of 0.5 ml enzymatic extract and after incubation at 37°C for 20 min, the absorbance was recorded at k = 340 nm using spectrophotometer (Biochrom Ultrospec II, LKB, Sweden). D1-pyrroline-5-carboxylated reductase (P5CR; EC 1.5.1.2) activity was measured in the mixture containing 0.06 mmol dm -3 NADH, 0.15 mmol dm -3 D1-pyrroline-5-carboxylic acid, 120 mmol dm -3 KP buffer, 2 mmol dm -3 dithiothreitol and enzyme extract and the decrease in the absorbance was followed at k = 340 nm (Madan et al 1995). Ornithine-D-aminotransferase (OAT; EC 2.6.1.68) was assayed by the method of Vogel and Kopac (1960).…”

Section: Proline Analysismentioning

confidence: 99%

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

et al. 2012

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Contents of ethylene, osmoprotectants, levels and forms of polyamines (PAs) and activities of antioxidant enzymes in the leaves and roots were investigated for five wheat cultivar seedlings (differing in drought tolerance) exposed to osmotic stress (-1.5 MPa). Stress was induced by 2-day-long treatment of plants with polyethylene glycol 6000 (PEG) or NaCl added to hydroponic cultures. Nawra, Parabola and Manu cv. (drought tolerant) showed a marked increase in osmoprotectors (proline and soluble carbohydrates, mainly glucose, saccharose and maltose), free PAs (putrescine Put, spermidine Spd and spermine Spm) and Spd-conjugated levels, in both leaves and roots, after PEGtreatments. Radunia and Raweta (drought sensitive) exhibited smaller changes in the content of these substances. The analysis of enzymes involved in proline metabolism revealed the glutamate as a precursor of proline synthesis in PEGinduced stress conditions. The increase in the activity of antioxidative enzymes, especially catalase and peroxidases, was characteristic for tolerant wheat plants, but for sensitive ones, a decrease in superoxide dismutase and an increase in mainly glutathione reductase activities were observed. After NaCl-treatment smaller changes of all biochemical parameters were registered in comparison with PEG-induced stress. Exceptions were the higher values of ethylene content and a significant increase in saccharose, raffinose and maltose levels (only in stress sensitive plants). The proline synthesis pathway was stimulated from both glutamate and ornithine precursors. These results suggest that the accumulation of inorganic ions in NaCl-stressed plants may be involved in protective mechanisms as an additional osmoregultor. Thus, a weaker stressogenic effect as determined as water deficit by leaf relative water content and relative dry weight increase rate and differences in metabolite synthesis in comparison with PEG stress was observed. Proline seems to be the most important osmo-protector in osmotic stress initiated by both PEG and NaCl. The synthesis of sugars and PAs may be stimulated in a stronger stress conditions (PEG).

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“…1, p. 151-157, Jan./March, 2005 foliar total e número de folhas, aumento A tendência a aumento no teor de prolina livre em plantas crescidas em condição de deficiência hídrica tem sido constatada pela pesquisa (Marur et al, 1994;Singh-sangwan et al, 1994;Kumar e Singh, 1998; Somal e Yapa, 1998). Acredita-se que aminoácidos livres, como a prolina, protejam os tecidos vegetais contra esses estresses por servirem como reserva de nitrogênio, osmo-soluto e protetor hidrofóbico de enzimas e estruturas celulares (Madan et al, 1995;Somal e Yapa, 1998). Navari-Izzo et al (1990), citados por Marur et al (1994), sugeriram que o aumento no teor de aminoácidos livres poderia contribuir na tolerância da planta à deficiência hídrica por meio de aumento no potencial osmótico, ou como reserva de nitrogênio, principalmente na síntese de enzimas específicas.…”

Section: Resultsunclassified

“…O ajuste osmótico é importante mecanismo de tolerância à seca, porque capacita a planta a continuar a expansão celular, realizar ajustes estomáticos e fotossintéticos, otimizar o crescimento vegetal e ter, em alguns casos, produção rentável (Heuer, 1994;Kumar e Singh, 1998). Em decorrência da sua importância no ajuste osmótico, a prolina é a molécula mais estudada em plantas submetidas a estresses abióticos (Heuer, 1994;Madan et al, 1995).…”

Section: Introductionunclassified

Crescimento, teor de partenolídeo e de prolina em plantas de <em>tanacetum parthenium</em> (l.) Schultz-Bip crescidas em substrato com diferentes teores de umidade

Carvalho¹,

Casali

Souza³

et al. 2005

Acta Sci. Agron.

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RESUMO.Com o objetivo de determinar o efeito do teor de umidade do substrato sobre o teor de partenolídeo na parte aérea das plantas, cultivou-se a artemísia [Tanacetum parthenium (L.) Schultz Bip.] em vasos de 5 L com substrato mantido a 100%, 90%, 70% ou 50% da capacidade de campo. Nos níveis de maior disponibilidade hídrica no substrato, verificou-se maior altura, número de folhas, área foliar total e teor de partenolídeo. No menor nível de disponibilidade de água no solo verificou-se redução de 16% na altura das plantas, de 13% no número de folhas, de 14% na área foliar total e de 28% no teor de partenolídeo, além de aumento no teor de prolina. Conclui-se que os maiores teores de umidade no substrato são mais indicados à produção de plantas com maior teor de partenolídeo.Palavras-chave: planta medicinal, deficiência hídrica, princípio ativo.ABSTRACT. Growth, parthenolide and proline contents in Tanacetum parthenium (L. ) Schultz Bip. in substratum of different humidity contents. Aiming at determining the effect of soil's water levels on the parthenolide contents of plants aerial parts, this experiment used feverfew [Tanacetum parthenium (L. ) Schultz Bip. ], grown in 5-L vases, with substratum kept at 100%, 90%, 70% and 50% field capacity. In the substratum highest water availability levels, plants showed larger height, more leaves, higher total leaf area and higher parthenolide content. In the lowest water availability level, the following decreases were verified: 16% in plants height, 13% in number of leaves, 14% in leaf area and 28% in parthenolide content, and an increase in the proline content. This suggests that higher water levels in soil are more appropriate for producing plants with larger parthenolide content.

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Proline and Proline Metabolising Enzymes in in-vitro Selected NaCl-tolerant Brassica juncea L. under Salt Stress

Cited by 125 publications

References 26 publications

The enhancement of drought tolerance for pigeon pea inoculated by arbuscular mycorrhizae fungi

The enhancement of drought tolerance for pigeon pea inoculated by arbuscular mycorrhizae fungi

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

Crescimento, teor de partenolídeo e de prolina em plantas de <em>tanacetum parthenium</em> (l.) Schultz-Bip crescidas em substrato com diferentes teores de umidade

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