Proline-rich protein MdPRP6 alters low nitrogen stress tolerance by regulating lateral root formation and anthocyanin accumulation in transgenic apple (Malus domestica)

Zhang, Xiaoli; Gong, Xiaoqing; Cheng, Siyuan; H, Yu; Li, Danyang; Su, Xinjian; Lei, Zhaolong; Li, Mingjun; Ma, Fengwang

doi:10.1016/j.envexpbot.2022.104841

Cited by 10 publications

(4 citation statements)

References 74 publications

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“…Previously we demonstrated that MdPRP6 was expressed in leaves, stems, and roots of apple plants, and it played a role in plants responding to heat, salt, and low nitrogen stresses (Zhang et al ., 2021a , 2022 , 2023 ). Here, we further examined the spatial expression patterns of MdPRP6 by in situ hybridization in different tissues.…”

Section: Resultsmentioning

confidence: 99%

“…To gain a better understanding of the role of MdPRP6 under drought stress, we generated another transgenic line with suppressed expression of MdPRP6 in GL‐3 via RNA interference silencing (Ri3 in Figure S6a,b ), without a decrease in expression levels of the other MdPRP members (Figure S6c , Ri1 and Ri2 were previously obtained in Zhang et al ., 2022 ). After 8 days of plant exposure to drought stress, leaves of WT were substantially more wilting than those of Ri plants (Figure 5a,b ).…”

Section: Resultsmentioning

confidence: 99%

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The ubiquitin‐binding protein MdRAD23D1 mediates drought response by regulating degradation of the proline‐rich protein MdPRP6 in apple (Malus domestica)

Zhang

Gong

et al. 2023

Plant Biotechnology Journal

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Summary RAD23 (RADIATION SENSITIVE23) proteins are a group of UBL‐UBA (ubiquitin‐like‐ubiquitin‐associated) proteins that shuttle ubiquitylated proteins to the 26S proteasome for breakdown. Drought stress is a major environmental constraint that limits plant growth and production, but whether RAD23 proteins are involved in this process is unclear. Here, we demonstrated that a shuttle protein, MdRAD23D1, mediated drought response in apple plants (Malus domestica). MdRAD23D1 levels increased under drought stress, and its suppression resulted in decreased stress tolerance in apple plants. Through in vitro and in vivo assays, we demonstrated that MdRAD23D1 interacted with a proline‐rich protein MdPRP6, resulting in the degradation of MdPRP6 by the 26S proteasome. And MdRAD23D1 accelerated the degradation of MdPRP6 under drought stress. Suppression of MdPRP6 resulted in enhanced drought tolerance in apple plants, mainly because the free proline accumulation is changed. And the free proline is also involved in MdRAD23D1‐mediated drought response. Taken together, these findings demonstrated that MdRAD23D1 and MdPRP6 oppositely regulated drought response. MdRAD23D1 levels increased under drought, accelerating the degradation of MdPRP6. MdPRP6 negatively regulated drought response, probably by regulating proline accumulation. Thus, “MdRAD23D1‐MdPRP6” conferred drought stress tolerance in apple plants.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The ubiquitin‐binding protein MdRAD23D1 mediates drought response by regulating degradation of the proline‐rich protein MdPRP6 in apple (Malus domestica)

Zhang

Gong

et al. 2023

Plant Biotechnology Journal

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“…These transformants have been utilized to examine nutrient uptake, abiotic stresses, nodule development, mycorrhizal interactions, allelopathy, biosynthesis, and wood formation. For instance, in apple, the function of the MdPRP6 gene was elucidated under low nitrogen conditions through A. rhizogenes -mediated knockout or overexpression ( Zhang et al., 2022 ). In Eucalyptus , the STOP-like gene was disrupted to assess its aluminum resistance function ( Sawaki et al., 2014 ).…”

Section: Applications In Woody Plantsmentioning

confidence: 99%

Agrobacterium rhizogenes: paving the road to research and breeding for woody plants

Ying,

Wen,

et al. 2023

Front. Plant Sci.

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Woody plants play a vital role in global ecosystems and serve as valuable resources for various industries and human needs. While many woody plant genomes have been fully sequenced, gene function research and biotechnological breeding advances have lagged behind. As a result, only a limited number of genes have been elucidated, making it difficult to use newer tools such as CRISPR-Cas9 for biotechnological breeding purposes. The use of Agrobacterium rhizogenes as a transformative tool in plant biotechnology has received considerable attention in recent years, particularly in the research field on woody plants. Over the past three decades, numerous woody plants have been effectively transformed using A. rhizogenes-mediated techniques. Some of these transformed plants have successfully regenerated. Recent research on A. rhizogenes-mediated transformation of woody plants has demonstrated its potential for various applications, including gene function analysis, gene expression profiling, gene interaction studies, and gene regulation analysis. The introduction of the Ri plasmid has resulted in the emergence of several Ri phenotypes, such as compact plant types, which can be exploited for Ri breeding purposes. This review paper presents recent advances in A. rhizogenes-mediated basic research and Ri breeding in woody plants. This study highlights various aspects of A. rhizogenes-mediated transformation, its multiple applications in gene function analysis, and the potential of Ri lines as valuable breeding materials

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“…Así mismo, la falta de nutrientes en el campo de cultivo puede ser un gran problema para el desarrollo de la las plantas, tal y como lo señala (Zhang et al, 2022) quien indica que el nitrógeno (N) al ser un nutriente esencial que regula muchos aspectos como el crecimiento y desarrollo de las plantas. Su presencia de forma natural en los campos de cultivo es cada vez más escasa y la aplicación de una gran cantidad de este fertilizante no sólo puede aumentar el costo de la producción agrícola, sino también tienen efectos nocivos sobre el medio ambiente.…”

Section: Estado Del Arteunclassified

Cultivos Transgénicos: El Futuro de la Agricultura

Marquina

2022

HIGH TECH-ENGINEERING JOURNAL

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El presente artículo analiza los aspectos referentes a los organismos modificados genéticamente y su implementación en la agricultura, en él se expone las características mejoradas de estos, así como los diversos beneficios que ofrecen ante diferentes problemas que se presentan en dicha área que es la agricultura. Mediante la revisión de algunos artículos los cuales aportan datos positivos y fiables con respecto a la modificación del genoma de cultivos convencionales y la utilización de dichos cultivos transgénicos, en el análisis se exponen las razones para la utilización de estos cultivos, tales como la capacidad de resistencia a plagas y enfermedades, resistencia a sequias, resistencia a metales pesados y a la falta de nutrientes en el terreno. Además de ello, se analizan los riesgos sobre la inocuidad para la salud de estos organismos y otros factores, en particular la contaminación genética de los cultivos no modificados genéticamente, los impactos sobre el medio ambiente como la aparición de resistencias en insectos, la pérdida de biodiversidad, el aumento de los productos químicos empleados. Por último, mediante la revisión de dichos artículos se concluye que los cultivos transgénicos son beneficioso y a su vez favorecen la agricultura, dado que poseen mejores características que ayudan a su crecimiento en comparación a los cultivos convencionales, lo que otorgan grandes beneficios para los agricultores al aumentar su producción y economía, así como también para el medio ambiente, al reducir el uso de tierras, pesticidas y las emisiones de gases de efecto invernadero. De igual manera beneficia a los consumidores con respecto a la seguridad alimentaria, ya que dichos, cultivos son evaluados arduamente, no sólo en su desempeño agronómico, sino en su inocuidad para garantiza que su consumo no causará ningún daño.

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Proline-rich protein MdPRP6 alters low nitrogen stress tolerance by regulating lateral root formation and anthocyanin accumulation in transgenic apple (Malus domestica)

Cited by 10 publications

References 74 publications

The ubiquitin‐binding protein MdRAD23D1 mediates drought response by regulating degradation of the proline‐rich protein MdPRP6 in apple (Malus domestica)

The ubiquitin‐binding protein MdRAD23D1 mediates drought response by regulating degradation of the proline‐rich protein MdPRP6 in apple (Malus domestica)

Agrobacterium rhizogenes: paving the road to research and breeding for woody plants

Cultivos Transgénicos: El Futuro de la Agricultura

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