2017
DOI: 10.1099/mic.0.000490
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Proline utilization system is required for infection by the pathogenic α-proteobacterium Brucella abortus

Abstract: Proline utilization (Put) systems have been described in a number of bacteria; however, the importance and functionality of the Put system in the intracellular pathogen Brucellaabortus has not been explored. Generally, bacterial Put systems are composed of the bifunctional enzyme proline dehydrogenase PutA and its transcriptional activator PutR. Here, we demonstrate that the genes putA (bab2_0518) and putR (bab2_0517) are critical for the chronic infection of mice by B. abortus, but putA and putR are not requi… Show more

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Cited by 9 publications
(13 citation statements)
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“…1B ) M2P media, showing that PutA is required for proline utilization by C. crescentus . This result strongly suggests that C. crescentus PutA can convert proline into glutamate as previously shown for PutA homologs from other Alphaproteobacteria 9 , 11 , 14 , 15 .
Figure 1 PutA and PutR are required for proline utilization as a carbon source.
…”
Section: Resultssupporting
confidence: 86%
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“…1B ) M2P media, showing that PutA is required for proline utilization by C. crescentus . This result strongly suggests that C. crescentus PutA can convert proline into glutamate as previously shown for PutA homologs from other Alphaproteobacteria 9 , 11 , 14 , 15 .
Figure 1 PutA and PutR are required for proline utilization as a carbon source.
…”
Section: Resultssupporting
confidence: 86%
“…Most bacteria use a bi-functional PutA enzyme to catabolize the conversion of proline into glutamate, the first step in proline utilization 3 . In order to study how C. crescentus uses proline as a source of nutrients, we searched for a putA homolog in its genome and found the CCNA_00846 protein that shares ~47% of identity with bi-functional PutA proteins from other Alphaproteobacteria 9 , 14 . It encodes a protein carrying two domains: one with similarities with the PRODH domain and the other one with similarities with the P5CDH domain, as expected for a bi-functional enzyme 3 .…”
Section: Resultsmentioning
confidence: 99%
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“…L‐Proline plays an important role in the virulence mechanism of various pathogens. Proline is the most hydrophilic amino acid and therefore it is found to accumulate in the cells of large variety of water‐stressed organisms and hence comes in the class of osmolytes . Proline used to stimulate the growth and respiration in bacteria in the presence of low water activities therefore act as proteogenic amino acid or “chaperone” to protein formation in the osmolytic conditions .…”
Section: Figurementioning
confidence: 99%