Prolongation of Discordant Heart Xenograft Survival after Liver Hemoperfusion: Role of Nonparenchymal Liver Cells

Militerno, Giuseppe; Gugenheim, Jean; Cursio, Raffaele; Hofman, Paul; Saint–Paul, Marie–Christine; Damais, Anne; Mouïel, J

doi:10.1159/000129543

Cited by 4 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that blocking KCs by GdCl 3 can reduce ischemia/reperfusion injury24–26 and have protective effects on grafts in LT 27–29. Nevertheless, other studies have revealed that KCs play a protective role in liver ischemia/reperfusion injury and transplantation,30–35 and the animal survival rate is significantly decreased because of KC blockade;32, 33 this is consistent with our findings.…”

Section: Discussionsupporting

confidence: 92%

Role of Kupffer cells in the induction of tolerance of orthotopic liver transplantation in rats

Chen¹,

Liu²,

Liang³

et al. 2008

Liver Transpl

View full text Add to dashboard Cite

Because the role of Kupffer cells (KCs) in liver transplantation (LT) tolerance is not well understood, we investigated their role in liver allograft acceptance in rats. Male Sprague-Dawley rats were randomly assigned to either an LT group or a transplantation group pretreated with GdCl 3 (Gd group). The rats were postoperatively sacrificed at indicated times for histology and assessment of KC function, nuclear factor kappa B (NF-B) activity, and cytokine production. KCs and T cells (TCs) were isolated from allografts to assess Fas/Fas ligand (FasL) expression. Cytotoxicity of KCs against TCs was monitored by coculturing of 3 H-thymidine TCs with KCs at various effector-to-target ratios. The results were as follows. First, grafts were spontaneously accepted in the LT group with evident apoptosis of TCs; however, inhibition of KCs by pretreatment with GdCl 3 decreased TC apoptosis and shortened the survival of allografts. Second, KCs in the LT group had increased levels of FasL messenger RNA and protein with respect to that in the Gd group. Third, by in vitro cocultivation assays, KCs induced TC apoptosis though elevated expression of FasL, and this process could be blocked by anti-FasL antibody. Fourth, there was a positive correlation between activation of NF-B and FasL expression in KCs and interleukin-4 production in the LT group, and the activation of NF-B was inhibited by pretreatment with GdCl 3 . In conclusion, KC-induced depletion of TCs via the Fas/FasL pathway might play a critical role in LT tolerance. However, the tolerance is abrogated by suppression of FasL and IL-4 expression via inhibition of NF-B activity by GdCl 3 . Liver Transpl 14: 823-836, 2008.

show abstract

Section: Discussionsupporting

confidence: 92%

Role of Kupffer cells in the induction of tolerance of orthotopic liver transplantation in rats

Chen¹,

Liu²,

Liang³

et al. 2008

Liver Transpl

View full text Add to dashboard Cite

show abstract

“…For example, intravenous MDP was used to activate guinea pig liver macrophages to absorb cytotoxic antibodies and protect heart xenografts [114].…”

Section: Macrophage Activation In Animal Modelsmentioning

confidence: 99%

Effects of Muramyl Peptides on Macrophages, Monokines, and Sleep

Pabst

Beranová-Giorgianni

Krueger

1999

Neuroimmunomodulation

View full text Add to dashboard Cite

Muramyl peptides are fragments of peptidoglycan from the cell walls of bacteria. Because of their unique chemistry, the immune system recognizes that muramyl peptides are products of bacteria, and it responds by becoming activated to resist infection. This resistance to infection is nonspecific, and extends to unrelated species of bacteria, fungi, and viruses. A key mechanism of the resistance to infection is activation of macrophages. Macrophage activation results in increased production of microbicidal oxygen radicals like superoxide and peroxide, and in increased secretion of inflammatory cytokines like interleukin-1β and tumor necrosis factor-α. These cytokines, besides activating neutrophils, B lymphocytes, and T lymphocytes, act on the central nervous system to induce physiological responses like fever and sleep. These physiological responses also aid in combating infection. Muramyl peptides also activate macrophages and other cells of the immune system to kill cancer cells. Muramyl peptides and similar agents will become more important as therapeutic agents in the future, due to increasing resistance of microbes to antibiotics, and increasing numbers of patients with immunodeficiencies.

show abstract