Prolongation of Islet Graft Survival Using Concomitant Transplantation of Islets and Vascular Endothelial Cells in Diabetic Rats

Song, Huanjin; Xue, Wujun; Li, Y.; Tian, Xin; Ding, Xiaoming; Xiong, Feng; Song, Yong; Tian, Puxun

doi:10.1016/j.transproceed.2010.06.003

Cited by 23 publications

(22 citation statements)

References 16 publications

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“…Previous reports have shown that endothelial (progenitor) cells of different origin can improve transplanted beta cell survival and function [7,8,16]. Nevertheless, in contrast to these studies, which evaluated the effect of non-human and/or human cord-blood-derived cells, the present study pioneers co-transplantation of adult human peripheral BOEC with a marginal-islet graft and reports a significant BOEC-specific increase in plasma C-peptide levels and improvement in blood glucose control.…”

Section: Discussionmentioning

confidence: 85%

“…Therefore, strategies that preserve early graft function and survival are needed, reducing the required number of islets per recipient. When cotransplanted, rat aortic endothelial cells have been shown to support beta cell function and/or survival [7]. Human cord-blood-derived endothelial progenitor cells (EPC) are beneficial to porcine islet graft transplantation via rapid graft revascularisation [8].…”

Section: Introductionmentioning

confidence: 99%

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Human blood outgrowth endothelial cells improve islet survival and function when co-transplanted in a mouse model of diabetes

et al. 2012

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Aims/hypothesis As current islet-transplantation protocols suffer from significant graft loss and dysfunction, strategies to sustain the long-term benefits of this therapy are required. Rapid and adequate oxygen and nutrient delivery by blood vessels improves islet engraftment and function. The present report evaluated a potentially beneficial effect of adult human blood outgrowth endothelial cells (BOEC) on islet graft vascularisation and function. Methods Human BOEC, 5×10 5 , were co-transplanted with a rat marginal-islet graft under the kidney capsule of hyperglycaemic NOD severe combined immunodeficiency (SCID) mice, and the effect on metabolic outcome was evaluated. Results Although vessel density remained unaffected, cotransplantation of islets with BOEC resulted in a significant and specific improvement of glycaemia and increased plasma C-peptide. Moreover, in contrast to control mice, BOEC recipients displayed reduced beta cell death and increases in body weight, beta cell proliferation and graft-vessel and beta cell volume. In vivo cell tracing demonstrated that BOEC remain at the site of transplantation and do not expand. The potential clinical applicability was underscored by the observed metabolic benefit of co-transplanting islets with BOEC derived from a type 1 diabetes patient. Conclusions/interpretation The present data support the use of autologous BOEC in translational studies that aim to improve current islet-transplantation protocols for the treatment of brittle type 1 diabetes.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Human blood outgrowth endothelial cells improve islet survival and function when co-transplanted in a mouse model of diabetes

et al. 2012

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“…In addition, a smaller islet mass is demanded to reverse hyperglycemia in the renal subcapsular space [61,62]. Furthermore, islet transplants under the kidney capsule allow the cotransplantion with endothelial cells [63], MSCs [64], and BM stem cells [65]. In humans, the invasive surgical procedure is used to release the islets under the kidney capsule, which is the really limited space for a high transplant mass.…”

Section: Kidney Capsulementioning

confidence: 99%

Xenotransplantation for Islets from Clinical Side

Wang¹,

Liang²,

Nie³

et al. 2017

Xenotransplantation - New Insights

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Islet transplantation can eliminate severe hypoglycemia symptoms caused by conventional treatment, and has the advantages of less trauma and complications, which is considered as the most promising treatment for type 1 diabetes mellitus (T1DM). Regulatory guidance is needed for a standard pig source. In section 1, the regulation of medical grade designed pathogen free (DPF) donor pig for clinical xenotransplantation consists of five parts: genetic quality control, microbiological surveillance, formula feeds, specification of pathological diagnosis, and requirements of environment and housing facilities. In section 2, we present the current approach and progress in pig donor selecting, pancreatic digestion, isolation and preparation of porcine islet grafts, identification and quality assessment of final islet product in clinical trials. The liver is currently the most preferred site for islet transplantation, even though it is far from ideal. A large number of alternative sites have been used for islet transplantation in experimental animal models to provide improved engraftment and long-term survival. In Section 3, we introduce some commonly used sites in xenotransplantation. The benefits and drawbacks of each parameter above are discussed in an attempt to decide which is the most suitable for clinical use and to direct future research.

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“…Concomitant transplantation of islets with BM stem cells, vascular endothelial cells, endothelial progenitor cells (EPCs) and MSCs creates a suitable niche for promotion of revascularization and enhancement of grafted islet survival and function (123,124,125,126).…”

Section: Enhancement Of Islet Vasculaturementioning

confidence: 99%

THERAPY OF ENDOCRINE DISEASE: Islet transplantation for type 1 diabetes: so close and yet so far away

Khosravi‐Maharlooei¹,

Hajizadeh‐Saffar²,

Tahamtani³

et al. 2015

European Journal of Endocrinology

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Over the past decades, tremendous efforts have been made to establish pancreatic islet transplantation as a standard therapy for type 1 diabetes. Recent advances in islet transplantation have resulted in steady improvements in the 5-year insulin independence rates for diabetic patients. Here we review the key challenges encountered in the islet transplantation field which include islet source limitation, sub-optimal engraftment of islets, lack of oxygen and blood supply for transplanted islets, and immune rejection of islets. Additionally, we discuss possible solutions for these challenges.

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Prolongation of Islet Graft Survival Using Concomitant Transplantation of Islets and Vascular Endothelial Cells in Diabetic Rats

Cited by 23 publications

References 16 publications

Human blood outgrowth endothelial cells improve islet survival and function when co-transplanted in a mouse model of diabetes

Human blood outgrowth endothelial cells improve islet survival and function when co-transplanted in a mouse model of diabetes

Xenotransplantation for Islets from Clinical Side

THERAPY OF ENDOCRINE DISEASE: Islet transplantation for type 1 diabetes: so close and yet so far away

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