Prolonged outbreak of adenovirus A31 in allogeneic stem cell transplant recipients

Swartling, Lisa; Allard, Annika; Törlén, Johan; Ljungman, Per; Mattsson, Jonas; Sparrelid, E.

doi:10.1111/tid.12443

Cited by 18 publications

(13 citation statements)

References 53 publications

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“…Although respiratory HAdVs are detectable year round (Fig. 1), the impressive HAdV disease potential is demonstrated in regional and institutional outbreaks of certain subtypes (192)(193)(194)(195)(196)(197).…”

Section: Other Carvs In Sot and Allogeneic Hctmentioning

confidence: 99%

Community-Acquired Respiratory Viruses in Transplant Patients: Diversity, Impact, Unmet Clinical Needs

2019

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SUMMARY Patients undergoing solid-organ transplantation (SOT) or allogeneic hematopoietic cell transplantation (HCT) are at increased risk for infectious complications. Community-acquired respiratory viruses (CARVs) pose a particular challenge due to the frequent exposure pre-, peri-, and posttransplantation. Although influenza A and B viruses have a top priority regarding prevention and treatment, recent molecular diagnostic tests detecting an array of other CARVs in real time have dramatically expanded our knowledge about the epidemiology, diversity, and impact of CARV infections in the general population and in allogeneic HCT and SOT patients. These data have demonstrated that non-influenza CARVs independently contribute to morbidity and mortality of transplant patients. However, effective vaccination and antiviral treatment is only emerging for non-influenza CARVs, placing emphasis on infection control and supportive measures. Here, we review the current knowledge about CARVs in SOT and allogeneic HCT patients to better define the magnitude of this unmet clinical need and to discuss some of the lessons learned from human influenza virus, respiratory syncytial virus, parainfluenzavirus, rhinovirus, coronavirus, adenovirus, and bocavirus regarding diagnosis, prevention, and treatment.

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Section: Other Carvs In Sot and Allogeneic Hctmentioning

confidence: 99%

Community-Acquired Respiratory Viruses in Transplant Patients: Diversity, Impact, Unmet Clinical Needs

2019

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“…Although variable, most studies reported that HAdV infection including viremia usually occurred within the first 100‐180 days after transplantation . Endogenous reactivation of latent virus was regarded as the major mode of infection; however, de novo infection in nosocomial and community settings could also occur . HAdV‐attributable mortality is approximately 1%; however, in disseminated cases, the rate may increase up to 80%‐100% .…”

Section: Introductionmentioning

confidence: 99%

Prospective monitoring of adenovirus infection and type analysis after allogeneic hematopoietic cell transplantation: A single‐center study in Korea

Kang

Park

Kim

et al. 2018

Transplant Infectious Dis

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“…In general, relatively mild viral infections in healthy individuals can be prolonged or more severe in immunocompromised children. If undetected they may spread among transplanted patients which could potentially lead to a clinical manifestation (Schwartz et al, 2011;Swartling et al, 2015;van der Doef et al, 2016). Interestingly, the mere presence of certain viruses in the gut both before HSCT (van Montfrans et al, 2015) and before or within 1 week after HSCT (Legoff et al, 2017) can be predictive of/predispose for the development of intestinal GVHD.…”

Section: Discussionmentioning

confidence: 99%

Broad Virus Detection and Variant Discovery in Fecal Samples of Hematopoietic Transplant Recipients Using Targeted Sequence Capture Metagenomics

et al. 2020

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Pediatric allogeneic hematopoietic stem cell transplantation (HSCT) patients often suffer from gastro-intestinal (GI) disease caused by viruses, Graft-versus-Host Disease (GVHD) or a combination of the two. Currently, the GI eukaryotic virome of HSCT recipients remains relatively understudied, which complicates the understanding of its role in GVHD pathogenicity. As decisions regarding immunosuppressive therapy in the treatment of virus infection or GVHD, respectively, can be completely contradicting, it is crucial to better understand the prevalence and relevance of viruses in the GI tract in the HSCT setting. A real time PCR panel for a set of specific viruses widely used to diagnose the most common causes of GI viral gastroenteritis is possibly insufficient to grasp the full extent of viruses present. Therefore, we applied the targeted sequence capture method ViroCap to residual fecal samples of 11 pediatric allogeneic HSCT recipients with GI symptoms and a suspicion of GVHD, to enrich for nucleic acids of viruses that are known to infect vertebrate hosts. After enrichment, NGS was applied to broadly detect viral sequences. Using ViroCap, we were able to detect viruses such as norovirus and adenovirus (ADV), that had been previously detected using clinical diagnostic PCR on the same sample. In addition, multiple, some of which clinically relevant viruses were detected, including ADV, human rhinovirus (HRV) and BK polyomavirus (BKV). Interestingly, in samples in which specific PCR testing for regular viral GI pathogens did not result in a diagnosis, the ViroCap pipeline led to the detection of viral sequences of human herpesvirus (HHV)-7, BKV, HRV, KI polyomavirus and astrovirus. The latter was an only recently described variant and showed extensive sequence mismatches with the applied real time PCR primers and would therefore not have been detected if tested. Our results indicate that target enrichment of viral nucleic acids through ViroCap leads to sensitive and broad possibly clinically relevant virus detection, including the detection of newer variants in clinical HSCT recipient samples. As such, ViroCap could be a useful detection tool clinically, but also in studying the associations between viral presence and GVHD.

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Prolonged outbreak of adenovirus A31 in allogeneic stem cell transplant recipients

Cited by 18 publications

References 53 publications

Community-Acquired Respiratory Viruses in Transplant Patients: Diversity, Impact, Unmet Clinical Needs

Community-Acquired Respiratory Viruses in Transplant Patients: Diversity, Impact, Unmet Clinical Needs

Prospective monitoring of adenovirus infection and type analysis after allogeneic hematopoietic cell transplantation: A single‐center study in Korea

Broad Virus Detection and Variant Discovery in Fecal Samples of Hematopoietic Transplant Recipients Using Targeted Sequence Capture Metagenomics

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