Prolonged platelet preservation by transient metabolic suppression

Badlou, Bahram Alamdary; IJseldijk, M.J.W.; Smid, WM; Akkerman, Jan Willem N.

doi:10.1111/j.1537-2995.2004.04022.x

Cited by 32 publications

(58 citation statements)

References 42 publications

(68 reference statements)

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“…11,30 We observed some increase in P-selectin and phosphatidyl serine expression on murine platelets after 48 hours of cold storage. In contrast, P-selectin and phosphatidyl serine expression on the cold-stored human platelets were similar to those of platelets stored at RT (Table 3).…”

Section: Discussionmentioning

confidence: 79%

Galactosylation does not prevent the rapid clearance of long-term, 4°C-stored platelets

Wandall

Hoffmeister

Sørensen

et al. 2008

Blood

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Cold storage of platelets for transfusion is desirable to extend platelet storage times and to prevent bacterial growth. However, the rapid clearance of coldstored platelets prevents their use. A novel method for preventing the rapid clearance of cold-stored platelets has previously been developed in a murine model. Cold storage induces the clustering and recognition of exposed ␤-Nacetylglucosamine (␤GlcNAc) on platelet surfaces. Glycosylation of ␤GlcNAc residues with uridine 5-diphosphogalactose (UDP-galactose) results in the normal survival of short-term (2 h) 0°C-stored murine platelets. Based on this finding, we developed a similar glycosylation process by adding UDP-galactose to human apheresis platelets. A phase 1 clinical trial was conducted transfusing radiolabeled autologous apheresis platelets stored for 48 hours at 4°C with or without pretreatment with UDP-galactose. In contrast to the murine study, galactosylation of human platelets did not prevent the accelerated platelet clearance routinely observed after 4°C storage. We next developed a murine model of platelet storage for 48 hours at 4°C and showed that UDP-galactose treatment of murine platelets also did not prevent their rapid clearance, in agreement with the human platelet study. We conclude that different mechanisms of clearance may exist for short-and long-term cold-stored platelets. (Blood. 2008;111:3249-3256)

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Section: Discussionmentioning

confidence: 79%

Galactosylation does not prevent the rapid clearance of long-term, 4°C-stored platelets

Wandall

Hoffmeister

Sørensen

et al. 2008

Blood

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“…Reduced glycolysis means that glucose remains present in the cold-stored PLTs throughout the 21-day storage period, which is at least 7 days beyond that of the conventionally stored PLTs, thus supporting an extension of the shelf life. Previous studies have also shown that metabolic suppression can result in preservation of PLT function, 50 which may also provide an explanation for the maintenance of aggregation in cold-stored PLTs. In addition, refrigerated storage reduces bacterial growth, 51,52 which is one of the primary reasons that the shelf life of PLTs is so limited.…”

Section: Discussionmentioning

confidence: 96%

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

et al. 2016

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“…28,29 To preserve platelet viability and provide optimal hemostatic effectiveness, an important drawback of storage of platelet concentrates at low temperature is the risk of spontaneous platelet activation, so-called coldinduced activation, which was also observed. 30e32 Coldinduced activation might partly explain GF release after storage of platelets at À70 C in the present study.…”

Section: Discussionmentioning

confidence: 98%

Freezing procedure without thrombin activation to retain and store growth factors from platelet concentrates

Gau

Shen

et al. 2011

Journal of Dental Sciences

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Background/purpose: The aim of this study was to examine a new procedure of simple freezing without the need of thrombin activation to retain and store growth factors (GFs) from platelet concentrates for up to 6 months. Materials and methods: After being re-suspended with Tyrode's solution, platelet suspensions were divided into four groups. In the negative control group, platelet supernatants were collected after centrifugation and then frozen at À70 C until being tested. In the frozen group, platelet suspensions were directly frozen and stored at À70 C; centrifugation was postponed until just before testing. In the thrombin and post-thrombin groups, the procedures were the same as those of the previous two groups, respectively, except that thrombin was added before centrifugation. Concentrations of platelet-derived GF-AB and tumor GF-b1 were assayed by an ELISA at 0 (the day on which the experiment began), 1, 2, and 4 months in first three groups (Experiment 1), and in all four groups at 6 months (Experiment 2). Results: Similar platelet-derived GF-AB and transforming GF-b1 concentrations were recorded in the frozen and thrombin groups with up to 4 months of storage. After 6 months of storage, similar concentrations were recorded among the frozen, thrombin, and post-thrombin groups.

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Prolonged platelet preservation by transient metabolic suppression

Abstract: Metabolic suppression before storage at 4 degrees C contributes to better preservation of PLT function.

Cited by 32 publications

References 42 publications

Galactosylation does not prevent the rapid clearance of long-term, 4°C-stored platelets

Galactosylation does not prevent the rapid clearance of long-term, 4°C-stored platelets

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

Freezing procedure without thrombin activation to retain and store growth factors from platelet concentrates

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