Prolonged SARS‐CoV‐2 RNA shedding: Not a rare phenomenon

Li, Na; Wang, Xiao; Lv, Tangfeng

doi:10.1002/jmv.25952

Cited by 191 publications

(203 citation statements)

References 8 publications

Supporting

Mentioning

186

Contrasting

Unclassified

Order By: Relevance

“…It is interesting to note that, with lower viremia, for the present case, 0.1 ≤ α ≤0.5 copies/ml, virus shedding events occur only when IIR= 0.1. The above provides an explanation for clinical observation related to positive RT-PCR results in patients who have recovered from COVID-19 22,23,24 . Following Li and collaborators 24 , the prolonged shedding of virus from recovered patients is obviously not an isolated phenomenon, but an integral component of the interaction dynamics between SARS-CoV-2 and the immune cells.…”

Section: Resultsmentioning

confidence: 82%

System Dynamics Modeling of Within-Host Viral Kinetics of Coronavirus (SARS CoV-2)

Burgos-Salcedo¹

2020

Preprint

View full text Add to dashboard Cite

Mathematical models are being used extensively in the study of SARS-CoV-2 transmission dynamics, becoming an essential tool for decision making concerning disease control. It is now required to understand the mechanisms involved in the interaction between the virus and the immune response effector cells, both innate and adaptive, in order to support lines of research related to the use of drugs, production of protective antibodies and of course, vaccines against SARS-CoV-2. The present study, using a system dynamic approach, hypothesizes over the conditions that characterize the fraction of the population which get infected by SARS-CoV-2 as the asymptomatic patients, the mild symptomatic, acute symptomatic, and also super-spreaders, in terms of innate immune response, the initial virus load, the virus burden with shedding events, and the cytokine levels.

show abstract

Section: Resultsmentioning

confidence: 82%

System Dynamics Modeling of Within-Host Viral Kinetics of Coronavirus (SARS CoV-2)

Burgos-Salcedo¹

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Importantly, 34.1 % of patients with E-,N2+ results had a previous diagnosis of COVID-19 and we observed an E-,N2+ result in a patient with an initial positive result obtained 71 days prior (e = 26.7; N2 = 29.0). These data support findings elsewhere [ 11 , 12 ] suggesting that low-level positive results can occur after an extended period in the same patient. It is therefore plausible that high Ct values, and in particular E-,N2+ results may occur among patients with prolonged sub-clinical infection or who have clinically recovered.…”

Section: Discussionmentioning

confidence: 99%

A needle in the haystack? Assessing the significance of envelope (E) gene-negative, nucleocapsid (N2) gene-positive SARS-CoV-2 detection by the Cepheid Xpert Xpress SARS-COV-2 assay

Khoshchehreh

Wald-Dickler

Holtom

et al. 2020

Journal of Clinical Virology

View full text Add to dashboard Cite

Highlights Controversy exists surrounding the clinical significance of high Crossing threshold (Ct) detections of SARS-CoV-2. Single target detection of SARS-CoV-2 (E-target-negative, N2-target positive) by the Cepheid Xpert Xpress SARS-Cov-2 accounted for 3.9 % of positive detections during a period of high COVID-19 activity (15.4 % overall positivity). Most patients with E-target-negative, N2-target-positive results were asymptomatic, but 25 % of those who underwent chest radiographic imaging had findings concerning for viral pneumonia suggesting subclinical infection. E-target-negative, N2-target-positive results were present in 8 symptomatic patients with a new diagnosis of COVID-19. Our findings suggest that the maximal PCR sensitivity is essential in hospital settings and that E-negative, N2-positive results can be significant.

show abstract

“…Based on previous estimates [ 9 , 20 ], respective high copy numbers are only detectable for few days of the infection. In contrast, highly sensitive PCR facilitates the detection of prolonged shedding of viral RNA sequences irrespective of their infectious potential [ 21–24 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the automated cartridge-based ARIES SARS-CoV-2 Assay (RUO) against automated Cepheid Xpert Xpress SARS-CoV-2 PCR as gold standard

Tanida

Koste

Koenig

et al. 2020

EuJMI

View full text Add to dashboard Cite

Introduction To evaluate the automated cartridge-based PCR approach ARIES SARS-CoV-2 Assay targeting the ORF-sequence and the N-gene of SARS-CoV-2. Methods In line with the suggestions by Rabenau and colleagues, the automated ARIES SARS-CoV-2 Assay was challenged with strongly positive samples, weakly positive samples and negative samples. Further, intra-assay and inter-assay precision as well as the limit-of-detection (lod) were defined with quantified target RNA and DNA. The Cepheid Xpert Xpress SARS-Cov-2 Assay was used as gold standard. Results Concordance between the ARIES assay and the Cepheid assay was 100% for strongly positive samples and for negative samples, respectively. For weakly positive samples as confirmed applying the Cepheid assay, a relevant minority of 4 out of 15 samples (26.7%) went undetected by the ARIES assay. Intra- and inter-assay precision were satisfactory, while the lod was in the 103 DNA copies/reaction-range, in the 103 virus copies/reaction-range, or in the 103–104 free RNA copies/reaction-range in our hands. Conclusions The automated ARIES assay shows comparable test characteristics as the Cepheid assay focusing on strongly positive and negative samples but a slightly reduced sensitivity with weakly positive samples. Decisions on diagnostic use should include considerations on the lod.

show abstract

Prolonged SARS‐CoV‐2 RNA shedding: Not a rare phenomenon

Cited by 191 publications

References 8 publications

System Dynamics Modeling of Within-Host Viral Kinetics of Coronavirus (SARS CoV-2)

System Dynamics Modeling of Within-Host Viral Kinetics of Coronavirus (SARS CoV-2)

A needle in the haystack? Assessing the significance of envelope (E) gene-negative, nucleocapsid (N2) gene-positive SARS-CoV-2 detection by the Cepheid Xpert Xpress SARS-COV-2 assay

Evaluation of the automated cartridge-based ARIES SARS-CoV-2 Assay (RUO) against automated Cepheid Xpert Xpress SARS-CoV-2 PCR as gold standard

Contact Info

Product

Resources

About