Prolonged storage of red blood cells affects aminophospholipid translocase activity

Verhoeven, Arthur J.; Hilarius, P. M.; Dekkers, David W. C.; Lagerberg, Johan W.M.; Korte, Dirk de

doi:10.1111/j.1423-0410.2006.00822.x

Cited by 78 publications

(76 citation statements)

References 23 publications

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“…Similar findings have been reported in studies of humans, in which only a small percentage of PS‐positive erythrocytes (3.5–4.5%) were detected after prolonged storage times of up to 7 weeks using similar techniques 11, 24, 25, 26. One possible explanation for why an increase in RBC PS expression was not detected is that the PS‐positive portions of the erythrocyte membrane could have been released from the cell and become microparticles in the unit.…”

Section: Discussionsupporting

confidence: 88%

Effects of Leukoreduction and Storage on Erythrocyte Phosphatidylserine Expression and Eicosanoid Concentrations in Units of Canine Packed Red Blood Cells

Muro

Lee

Ross

et al. 2017

Veterinary Internal Medicne

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BackgroundStorage of canine packed red blood cells (pRBCs) can increase erythrocyte phosphatidylserine (PS) expression and eicosanoid concentrations.Hypothesis/ObjectivesTo determine the effects of leukoreduction on erythrocyte PS expression and eicosanoid concentrations in stored units of canine pRBCs. Our hypothesis was that leukoreduction would decrease PS expression and eicosanoid concentrations.AnimalsEight healthy dogs.MethodsIn a cross‐over study, units of whole blood were leukoreduced (LR) or non‐LR and stored (10 and 21 days) as pRBCs. Samples were collected at donation, and before and after a simulated transfusion. PS expression was measured by flow cytometry, and concentrations of arachidonic acid (AA), prostaglandin F2α (PGF 2α), prostaglandin E2 (PGE 2), prostaglandin D2 (PGD 2), thromboxane B2 (TXB 2), 6‐keto‐prostaglandin F1α (6‐keto‐PGF 1α), and leukotriene B4 (LTB 4) were quantified by liquid chromatography–mass spectrometry.ResultsThere was no change in PS expression during leukoreduction, storage, and simulated transfusion for non‐LR and LR units. Immediately after leukoreduction, there was a significant increase in TXB 2 and PGF 2α concentrations, but during storage, these eicosanoids decreased to non‐LR concentrations. In both LR and non‐LR units, 6‐keto‐PGF 1α concentrations increased during storage and simulated transfusion, but there was no difference between unit type. There was no difference in AA, LTB 4, PGE 2, and PGD 2 concentrations between unit types.Conclusions and Clinical ImportanceLeukoreduction, storage, and simulated transfusion do not alter erythrocyte PS expression. Leukoreduction causes an immediate increase in concentrations of TXB 2 and PGF 2α, but concentrations decrease to non‐LR concentrations with storage. Leukoreduction does not decrease the accumulation of 6‐keto‐PGF 1α during storage.

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Section: Discussionsupporting

confidence: 88%

Effects of Leukoreduction and Storage on Erythrocyte Phosphatidylserine Expression and Eicosanoid Concentrations in Units of Canine Packed Red Blood Cells

Muro

Lee

Ross

et al. 2017

Veterinary Internal Medicne

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“…For example, decreased deformability of stored RBCs may be ameliorated by correction of intracellular pH and restoration of adenosine triphosphate levels (36,60). Repletion of SNO-Hb levels during storage improved the vasodilatory function of RBCs in vivo (41).…”

Section: Mechanisms Linking Blood Transfusion With Excess Mortalitymentioning

confidence: 96%

Bleeding, Blood Transfusion, and Increased Mortality After Percutaneous Coronary Intervention

Doyle

Rihal

Gastineau

et al. 2009

Journal of the American College of Cardiology

379

229

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Advances in percutaneous coronary intervention (PCI) during the past decade have led to more widespread use of these procedures in older and sicker patients. Refinement of periprocedural antithrombotic therapy has played a particularly important role in reducing ischemic complications to very low levels in routine practice. Although the use of more powerful antiplatelet agents has been associated with increased risk of bleeding (especially among the elderly and patients with serious comorbidities), such complications have traditionally been viewed as benign in nature. Recent studies, however, have identified major bleeding after PCI as an important predictor of increased mortality. Whether this relationship between bleeding and risk of death is cause-and-effect, or merely an association based on shared risk factors, remains unclear. In this review, we examine the basis for a possible causal link between post-PCI bleeding and subsequent mortality. Possible mechanisms underpinning such a link are discussed, including a potential adverse role for blood transfusion in this setting. A framework for further clinical evaluation of this issue is presented.

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“…16 This process is pH dependent and its rate increases with decreases in intracellular pH. 27 Verhoeven and colleagues 27 observed that storage of RBCs leads to a decrease in intracellular pH; intracellular pH of 7-week-stored human RBCs was as low as 6.36 while this was 6.83 in fresh human RBCs. This pH dependency might explain the differences between the hypoxia-induced NO formation rate in fresh and aged RBCs observed in this study.…”

Section: Discussionmentioning

confidence: 97%

“…24 Earlier studies have demonstrated biochemical, structural, and functional changes of RBCs induced by extended cold storage. 19,22,[25][26][27][28] Despite lack of strong evidence, it is believed that depletion of NO bioavailability in relatively "aged" RBCs may provide an explanation for the functional loss of transfused aged RBCs. Several mechanisms have been described to explain such changes, such as depletion of S-nitrosohemoglobin or increasing amounts of cell-free Hb due to hemolysis during the storage process.…”

Section: Discussionmentioning

confidence: 98%

Red blood cell storage increases hypoxia‐induced nitric oxide bioavailability and methemoglobin formation in vitro and in vivo

et al. 2014

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Prolonged storage of red blood cells affects aminophospholipid translocase activity

Abstract: This study shows that, although PS exposure remains low, prolonged storage does compromise the RBC membrane by affecting flippase activity. When the metabolic changes induced by storage are corrected, flippase activity can be restored.

Cited by 78 publications

References 23 publications

Effects of Leukoreduction and Storage on Erythrocyte Phosphatidylserine Expression and Eicosanoid Concentrations in Units of Canine Packed Red Blood Cells

Effects of Leukoreduction and Storage on Erythrocyte Phosphatidylserine Expression and Eicosanoid Concentrations in Units of Canine Packed Red Blood Cells

Bleeding, Blood Transfusion, and Increased Mortality After Percutaneous Coronary Intervention

Red blood cell storage increases hypoxia‐induced nitric oxide bioavailability and methemoglobin formation in vitro and in vivo

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