Prolonged Survival of Rat Liver Allografts Transfected With Fas-Ligand Expressing Plasmid

Cell surface expression of MHC class I antigen is suppressed in indoleamine 2,3-dioxygenase genetically modified keratinocytes: implications in allogeneic skin substitute engraftment

“…When compared with the systemic use of immunosuppressive drugs, local expression of immunomodulatory or graftprotecting molecules has the potential to eliminate problems associated with increased incidence of malignancy and susceptibility to opportunistic infections. Several local immunosuppressive factors including interleukin-10 (IL-10) [1], tumor growth factor-␤ (TGF-␤) [2], and Fas ligand (FasL) [3] have been studied for this aim. Despite advances made in the past decade, these molecules have low therapeutic efficiency.…”

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

“…Using this vector, Bcl2 transfection into the rat liver graft was able to inhibit apoptosis after ischemia reperfusion injury (38,39), and a FasL transfected liver graft could eliminate the infiltrated cytotoxic lymphocytes efficiently (40). These previous results suggested that the HVJ-liposome vector mediated gene delivery system might therefore be a suitable treatment modality in the transplant setting.…”

Genetic Heterogeneity and Efficiency of Two Different Methods of Adenovirus-Mediated Gene Transfer in a Rat Liver Transplantation Model

“…18 Current progress in gene transfer has allowed intervention at the molecular level using gene delivery systems such as viral vector and liposome. [18][19][20] Initial experiments showed that direct injection of a plasmid encoding β-galactosidase gene into heterotopic cardiac grafts results in successful gene expression following transplantation in syngeneic recipients. 21 Retroviral mediated gene delivery via portal perfusion with intraparenchymal injection produced permanent expression of LacZ in regenerating reduced-size, syngeneic liver grafts.…”