Prolonging photoperiod promotes testosterone synthesis of Leydig cells by directly targeting local melatonin system in rooster testes

Xu, Gaoqing; Yuan, Zhiyu; Hou, Jiani; Zhao, Jing; Liu, Hongyu; Lu, Wenfa; Wang, Jun

doi:10.1093/biolre/ioab155

Cited by 8 publications

(8 citation statements)

References 64 publications

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“…Previous studies have shown that melatonin promotes testosterone synthesis in sheep and goat Leydig cells [8,9]. Our previous studies identified the melatonin receptor types of roosters, and our results demonstrated that melatonin could bind to MEL1A and MEL1B to inhibit testosterone synthesis through the cAMP/PKA/CREB signaling pathway in rooster Leydig cells [10]. These results showed that melatonin is species-specific in regulating testosterone synthesis in different species.…”

Section: Introductionsupporting

confidence: 69%

Melatonin Inhibits Testosterone Synthesis in Rooster Leydig Cells by Targeting CXCL14 through miR-7481-3p

Xu,

Pu,

Teng

et al. 2023

IJMS

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Melatonin has been proved to be involved in testosterone synthesis, but whether melatonin participates in testosterone synthesis by regulating miRNA in Leydig cells is still unclear. The purpose of this study is to clarify the mechanism of melatonin on Leydig cells testosterone synthesis from the perspective of miRNA. Our results showed that melatonin could significantly inhibit testosterone synthesis in rooster Leydig cells. miR-7481-3p and CXCL14 were selected as the target of melatonin based on RNA-seq and miRNA sequencing. The results of dual-luciferase reporter assays showed that miR-7481-3p targeted the 3′-UTR of CXCL14. The overexpression of miR-7481-3p significantly inhibited the expression of CXCL14 and restored the inhibitory role of melatonin testosterone synthesis and the expression of StAR, CYP11A1, and 3β-HSD in rooster Leydig cells. Similarly, interference with CXCL14 could reverse the inhibitory effect of melatonin on the level of testosterone synthesis and the expression of StAR, CYP11A1, and 3β-HSD in rooster Leydig cells. The RNA-seq results showed that melatonin could activate the PI3K/AKT signal pathway. Interference with CXCL14 significantly inhibited the phosphorylation level of PI3K and AKT, and the inhibited PI3K/AKT signal pathway could reverse the inhibitory effect of CXCL14 on testosterone synthesis and the expression of StAR, CYP11A1 and 3β-HSD in rooster Leydig cells. Our results indicated that melatonin inhibits testosterone synthesis by targeting miR-7481-3p/CXCL14 and inhibiting the PI3K/AKT pathway.

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Section: Introductionsupporting

confidence: 69%

Melatonin Inhibits Testosterone Synthesis in Rooster Leydig Cells by Targeting CXCL14 through miR-7481-3p

Xu,

Pu,

Teng

et al. 2023

IJMS

Self Cite

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“…With regard to poultry (such as chicken) ( 40 ), successive digestion of trypsin and collagenase II was carried out in the acquisition of cell suspension. In poultry, collagenase II digestion combined with differential centrifugation was applied in the isolation of rooster LCs, and passage was used in its purification ( 34 ). The contradistinction in digestive enzyme selection is probably owing to the distinct concentration of connective tissues in viviparous and oviparous animals ( 41 ), in which potent trypsin could first digest the connective tissues in chicken testes and then released conglobate seminiferous tubule.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the studies related to the isolation and culture of LCs were mainly focused on mice ( 28 ), rats ( 29 ), pigs ( 30 ), sheep ( 31 ), cows ( 32 ), and other mammals ( 33 ). In poultry, collagenase II digestion combined with differential centrifugation was applied in the isolation of rooster LCs, and passage was used in its purification ( 34 ). In contrast, the isolation and identification of highly purified primary LCs in ducks have received very little attention.…”

Section: Introductionmentioning

confidence: 99%

Primary culture and endocrine functional analysis of Leydig cells in ducks (Anas platyrhynchos)

Chu

Javed

Ashraf³

et al. 2023

Front. Endocrinol.

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Testicular Leydig cells (LCs) are the primary known source of testosterone, which is necessary for maintaining spermatogenesis and male fertility. However, the isolation, identification, and functional analysis of testosterone in duck LCs are still ambiguous. The aim of the present study was to establish a feasible method for isolating highly purified primary duck LCs. The highly purified primary duck LCs were isolated from the fresh testes of 2-month-old ducks via the digestion of collagenase IV and Percoll density gradient centrifugation; hematoxylin and eosin (H&E), immunohistochemistry (IHC) staining, ELISA, and radioimmunoassay were performed. Results revealed that the LCs were prominently noticeable in the testicular interstitium of 2-month-old ducks as compared to 6-month-old and 1-year-old ducks. Furthermore, IHC demonstrated that the cultured LCs occupied 90% area of the petri dish and highly expressed 3β-HSD 24 h after culture (hac) as compared to 48 and 72 hac. Additionally, ELISA and radioimmunoassay indicate that the testosterone level in cellular supernatant was highly expressed in 24 and 48 hac, whereas the testosterone level gradually decreased in 72 and 96 hac, indicating the primary duck LCs secrete testosterone at an early stage. Based on the above results, the present study has effectively developed a technique for isolating highly purified primary duck LCs and identified its biological function in synthesizing testosterone.

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“…The effects of melatonin on the levels of reproductive hormones depend on physiological conditions and species of animals 35 . Melatonin inhibits StAR, GATA-4/SF-1, or other protein expressions through specific binding sites by blocking StAR protein expression without altering the activity of the P450 enzyme 36 – 38 . Meanwhile, melatonin promotes male reproductive performance and increases testosterone synthesis in mammalian Leydig cells 39 .…”

Section: Discussionmentioning

confidence: 99%

The aphrodisiac potential of β-cyclodextrin–curcumin via stimulating cAMP-PKA pathway in testicular Leydig cells

Liu

Xue

Yuan

et al. 2022

Sci Rep

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The water-soluble β-cyclodextrin–curcumin (CDC) is used in pharmaceutical applications and as a natural food colorant. The previous study revealed that curcumin potentially impacted the reproductive system. The present study investigated the possible roles of the CDC in testosterone secretion in Leydig cells and mice. Primary Leydig cells were treated with the CDC to determine their effect on cell proliferation, testosterone levels, the protein and mRNA expression of the transcription factor, and steroidogenic enzymes. Our data showed that CDC stimulated testosterone production via upregulating transcription factor steroidogenic factor-1 (NR5A1), cAMP-response element-binding protein (CREB), and steroidogenic enzymes steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (CYP11A1), 17-alpha-hydroxylase/17,20-lyase (CYP17A1), 3β-/17β-hydroxysteroid dehydrogenase type 1 (3β/17β-HSD, HSD3b1/HSD17b1). CDC could significantly stimulate H89-suppressed StAR and CREB expression but not reverse melatonin-suppressed StAR expression. We further detected the hormonal activity with transgenic yeast, and CDC showed potential androgenic antagonistic activity. Meanwhile, we investigated its aphrodisiac effect on hydrocortisone-induced mice. Exposure to hydrocortisone decreased the mating ability, reproductive organs, and testosterone level and disrupted testicular histology. However, all of these effects were significantly improved by CDC treatment. In conclusion, these results indicated that mechanisms of CDC in stimulating testosterone production involve upregulating the cAMP-PKA pathway.

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Prolonging photoperiod promotes testosterone synthesis of Leydig cells by directly targeting local melatonin system in rooster testes

Cited by 8 publications

References 64 publications

Melatonin Inhibits Testosterone Synthesis in Rooster Leydig Cells by Targeting CXCL14 through miR-7481-3p

Melatonin Inhibits Testosterone Synthesis in Rooster Leydig Cells by Targeting CXCL14 through miR-7481-3p

Primary culture and endocrine functional analysis of Leydig cells in ducks (Anas platyrhynchos)

The aphrodisiac potential of β-cyclodextrin–curcumin via stimulating cAMP-PKA pathway in testicular Leydig cells

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