Prolonging somatic cell proliferation through constitutive hox gene expression in C. elegans

Heinze, Svenia D.; Berger, Simon; Engleitner, Stefanie; Daube, Michael; Hajnal, Alex

doi:10.1038/s41467-023-42644-1

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…Posterior intestinal cells distinguish themselves from more anterior cells in particular by the expression of two Hox transcription factors, NOB-1 and PHP-3 (Liu and Murray, 2023;Murray et al, 2022;Zhao et al, 2010). Interestingly, Hox family members have been shown to promote proliferation in C. elegans (Heinze et al, 2023;Roiz et al, 2016), raising the possibility that the presence of NOB-1 and/or PHP-3 in posterior cells could contribute to their ability to undergo supernumerary divisions in the absence of ELT-2 and ELT-7.…”

Section: Discussionmentioning

confidence: 99%

The proliferation and differentiation programs are only partially coupled duringC. elegansintestine development

Dieng,

Michaux,

Pacquelet

2024

Preprint

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Cell proliferation and differentiation are essential processes underlying multicellular organism development. Cell proliferation arrest usually precedes terminal differentiation, suggesting that these two processes may be coordinated. Here we took advantage of the very stereotyped development of theC. elegansintestine to address whether the control of the proliferation and differentiation programs are systematically coupled. We show that retarding cell cycle arrest leads to a delay in the recruitment of some, but not all, brush border components. Reciprocally, we find that cell cycle arrest relies on the differentiation factors ELT-2 and ELT-7 only in posterior intestinal cells. The occurrence of supernumerary divisions in the absence of ELT-2 and ELT-7 is associated with changes in the expression pattern of the cell cycle regulators CKI-1 and cyclin B1. Our work thus demonstrates the existence of reciprocal interactions between cell proliferation and cell differentiation. It nevertheless also shows that these two processes are only partially coupled, suggesting the existence of additional mechanisms ensuring their temporal control.

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Section: Discussionmentioning

confidence: 99%

The proliferation and differentiation programs are only partially coupled duringC. elegansintestine development

Dieng,

Michaux,

Pacquelet

2024

Preprint

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Hoxc10‐mediated ‘positional memory’ regulates cartilage formation subsequent to femoral heterotopic grafting

Song,

Hao,

Xie

et al. 2024

J Cellular Molecular Medi

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The Hox gene plays a crucial role in the bone development, determining their structure and morphology. Limb bone grafts expressing Hox positive genes are commonly used for free transplantation to repair Hox negative mandibular critical bone defects. However, the specific role of original Hox genes in newly formed bone during the cross‐layer bone grafting healing process remains unexplored. Our findings demonstrate that femurs ectopically grafted into the mandibular environment retained a significant ability to differentiate into cartilage and form cartilaginous callus, which may be a key factor contributing to differences in bone graft healing. Hoxc10, an embryonic layer‐specific genes, regulates cartilage formation during bone healing. Mechanistically, we observed Hoxc10 retention in co‐cultured femoral BMSCs. Knocking out Hoxc10 narrows the bone gap and reduces cartilage formation. In summary, we reveal Hoxc10's ‘positional memory’ after adult cross‐layer bone graft, influencing the outcomes of autologous bone graft.

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The role of lin-12 notch in C. elegans anchor cell proliferation

Hajnal,

Deng,

Lattmann

2024

Biology Open

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The gonadal anchor cell (AC) is an essential organizer for the development of the egg-laying organ in the C. elegans hermaphrodite. Recent work has investigated the mechanisms that control the quiescent state the AC adopts while fulfilling its functions. In this context, the transcription factors EGL-43 and NHR-67 are required to maintain the G1 cell cycle arrest of the AC and prevent proliferation. While NHR-67 acts primarily by up-regulating the CDK inhibitor CKI-1, the role of EGL-43 in this process has been subject to debate. Deng et al. (2020) reported that inhibition of the notch receptor lin-12 by RNAi partially suppressed the AC proliferation phenotype caused by egl-43 RNAi. By contrast, Martinez et al. (2022) found that down-regulation of LIN-12 NOTCH via the auxin-inducible degradation system did not reduce AC proliferation. To resolve this issue, we performed egl-43 RNAi in the background of a lin-12 null allele and observed a similar suppression of AC proliferation as reported previously by Deng et al. (2020). Hence, AC proliferation caused by the downregulation of egl-43 partially depends on LIN-12 NOTCH signaling.

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Prolonging somatic cell proliferation through constitutive hox gene expression in C. elegans

Cited by 3 publications

References 68 publications

The proliferation and differentiation programs are only partially coupled duringC. elegansintestine development

The proliferation and differentiation programs are only partially coupled duringC. elegansintestine development

Hoxc10‐mediated ‘positional memory’ regulates cartilage formation subsequent to femoral heterotopic grafting

The role of lin-12 notch in C. elegans anchor cell proliferation

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