“…Resultants were subjected to chemical as well as spectral analysis (Fig. 1) such as UV, IR, 1 HNMR, 13 CNMR and LCMS [10,11]. Fig.…”
Section: Methodsmentioning
confidence: 99%
“…Extensive chromatography of this extract sequestered asarinin, seasamin, sesamolin, simplexolin [9] and triacontanoic ester of 5ʹʹ-hydroxyjustisolin. Recently, the petroleum ether extract was found to have promising antitumor activity in vivo , by inhibiting angiogenesis via downregulation of VEGF and also by preventing the migration of cells [10].…”
“…Resultants were subjected to chemical as well as spectral analysis (Fig. 1) such as UV, IR, 1 HNMR, 13 CNMR and LCMS [10,11]. Fig.…”
Section: Methodsmentioning
confidence: 99%
“…Extensive chromatography of this extract sequestered asarinin, seasamin, sesamolin, simplexolin [9] and triacontanoic ester of 5ʹʹ-hydroxyjustisolin. Recently, the petroleum ether extract was found to have promising antitumor activity in vivo , by inhibiting angiogenesis via downregulation of VEGF and also by preventing the migration of cells [10].…”
“…HaCaT cells were seeded at 3.5 × 10 4 cells per well in 96-well plates and then treated with EGCG-5′Glu for 24 h. For cell proliferation measurements, HaCaT cells were seeded at 3 × 10 3 cells per well in 96-well plates and then treated with EGCG-5′Glu (0–25 μM) for 24 h and 48 h. After incubation times of 24 h and 48 h, cell viability and proliferation were measured using conventional MTT assay and trypan blue dye exclusion assay, as reported previously [ 43 , 44 ].…”
Epigallocatechin gallate (EGCG) is a well-studied polyphenol with antioxidant effects. Since EGCG has low solubility and stability, many researchers have modified EGCG residues to ameliorate these problems. A novel EGCG derivative, EGCG-5′-O-α-glucopyranoside (EGCG-5′Glu), was synthesized, and its characteristics were investigated. EGCG-5′Glu showed antioxidant effects in cell and cell-free systems. Under SNP-derived radical exposure, EGCG-5′Glu decreased nitric oxide (NO) production, and recovered ROS-mediated cell viability. Moreover, EGCG-5′Glu regulated apoptotic pathways (caspases) and cell survival molecules (phosphoinositide 3-kinase (PI3K) and phosphoinositide-dependent kinase 1 (PDK1)). In another radical-induced condition, ultraviolet B (UVB) irradiation, EGCG-5′Glu protected cells from UVB and regulated the PI3K/PDK1/AKT pathway. Next, the proliferative effect of EGCG-5′Glu was examined. EGCG-5′Glu increased cell proliferation by modulating nuclear factor (NF)-κB activity. EGCG-5′Glu protects and repairs cells from external damage via its antioxidant effects. These results suggest that EGCG-5′Glu could be used as a cosmetics ingredient or dietary supplement.
“…Sus resultados se interpretan dependiendo del contexto en que se plantea el objetivo de la investigación, si la sustancia evaluada no es citotóxica es un indicador que esta sustancia puede ser bien tolerada por un sistema biológico, y si la sustancia es citotóxica es un indicador que esta sustancia puede contener compuestos bioactivos con potencial efecto antitumoral (9) . Es importante señalar que un estudio desarrollado por el National Cancer Institute de los Estados Unidos mostró que existe una fuerte correlación entre la prueba de letalidad de Artemia salina, y la inhibición del crecimiento in vitro en líneas celulares de tumores sólidos humanos (10) ; estos resultados han servido para que, actualmente, varios estudios utilicen esta prueba para establecer un mecanismo de selección preliminar de especies vegetales con potencial anticancerígeno (11,12) .…”
Objetivo. Determinar la bioactividad de trece plantas medicinales peruanas a través de su capacidad citotóxica. Materiales y métodos. Se elaboraron extractos acuosos, hidroalcohólicos, o zumos liofilizados de las especies vegetales seleccionadas. La citotoxicidad in vitro fue evaluada usando la prueba de letalidad de Artemia salina, con la determinación de la concentración letal media (CL50). El potencial citotóxico de las muestras de extractos evaluados, se clasificaron en: a) no tóxico: CL50 > 1000 μg/ mL; b) baja toxicidad: 500 < CL50 ≤ 1000 μg/ mL; c) toxicidad moderada: 100 < CL50 ≤ 500 μg/ mL, y d) alta toxicidad: CL50 < 100 μg/ mL. Resultados. Los diferentes extractos del rizoma de Curcuma longa mostraron una potente actividad citotóxica, con CL50 entre 20,67 ± 7,04 y 98,14± 2,64 ug/mL. Los extractos de rizoma de Zingiber officinale, del fruto de Physalis angulata y la planta entera de Physalis angulata también mostraron actividad citotóxica con CL50 de 87,15±18,17, 323,48±18,85y 328,92±23,08 ug/mL, respectivamente. Conclusión. Se encontró actividad citotóxica en losextractos de los rizomas de Curcuma longa, Zingiber officinale, así como el fruto y planta entera dePhysalis angulata. Futuros estudios podrán determinar si la flora cultivada en el Perú puede ser unafuente para el desarrollo futuro de agentes antitumorales.
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