Promising antimicrobial and antibiofilm activities of Orobanche aegyptiaca extract-mediated bimetallic silver-selenium nanoparticles synthesis: Effect of UV-exposure, bacterial membrane leakage reaction mechanism, and kinetic study

Mostafa, H. Y.; El-Sayyad, Gharieb S.; Nada, Hanady G.; Ellethy, Rania A.; Zaki, E. G.

doi:10.1016/j.abb.2023.109539

Cited by 24 publications

(9 citation statements)

References 96 publications

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“…The color of the WR seemed yellow, which shifted to a deep reddish brown concerning the biosynthesis of bimetallic Se-Ag NPs. The produced color was assigned to the stimulation of biogenic Se-Ag NPs’ surface plasmon resonance and provided a specific wavelength of their appearance [ 40 ]. The capacity of WR filtrate to produce bimetallic Se-Ag NPs was evaluated.…”

Section: Resultsmentioning

confidence: 99%

“…The anisotropic form and partial stability of the NPs in our work are caused by using of reducing and capping agents (WR extract). Finally, the outcomes were connected to the most recent publications [ 40 ]. Bimetallic Se-Ag NPs were subjected to DLS analysis in order to assess the particle size distribution and determine the average particle size, which was determined to be 47.3 nm ( Figure 2 d).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, both E. coli and C. albicans were the least sensitive among the other tested microbial strains, where the MIC was 50 µg/mL for each one. Mostafa, El-Sayyad [ 40 ] used Orobanche aegyptiaca extract for the biosynthesis of bimetallic Ag-Se NPs where it showed promising antimicrobial activity against S. aureus , E. coli , P. aeruginosa , and C. albicans. Also, bimatellic Ag-Se nanoparticles were synthesized using quercetin and gallic acid, where exhibited outstanding antimicrobial and antitumor activities [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

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Watermelon Rind Mediated Biosynthesis of Bimetallic Selenium-Silver Nanoparticles: Characterization, Antimicrobial and Anticancer Activities

Hashem,

El-Sayyad,

Al-Askar

et al. 2023

Plants

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One of the most hazardous diseases that influences human health globally is microbial infection. Therefore, bimetallic nanoparticles have received much attention for controlling microbial infections in the current decade. In the present study, bimetallic selenium–silver nanoparticles (Se-Ag NPs) were effectively biosynthesized using watermelon rind WR extract through the green technique for the first time. UV-visible spectroscopy, transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDX) methods were used to characterize the produced NPs. The results indicated that the bimetallic Se-Ag NPs had synergistic antimicrobial activity at low concentrations, which helped to reduce the toxicity of Ag NPs after the bimetallic Se-Ag NPs preparation and increase their great potential. Se-Ag NPs with sizes ranging from 18.3 nm to 49.6 nm were detected by TEM. Se-Ag NP surfaces were uniformly visible in the SEM picture. The cytotoxicity of bimetallic Se-Ag NPs was assessed against the Wi38 normal cell line to check their safety, where the IC50 was 168.42 µg/mL. The results showed that bimetallic Se-Ag NPs had antibacterial action against Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Bacillus subtilis, and Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 12.5 to 50 µg/mL. Additionally, bimetallic Se-Ag NPs had promising anticancer activity toward the MCF7 cancerous cell line, where the IC50 was 21.6 µg/mL. In conclusion, bimetallic Se-Ag NPs were biosynthesized for the first time using WR extract, which had strong antibacterial, antifungal and anticancer properties.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Watermelon Rind Mediated Biosynthesis of Bimetallic Selenium-Silver Nanoparticles: Characterization, Antimicrobial and Anticancer Activities

Hashem,

El-Sayyad,

Al-Askar

et al. 2023

Plants

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“…These nanoparticles exhibited noteworthy antibacterial properties against MDR E. coli and methicillin-resistant Staphylococcus aureus (MRSA) strains ( Lomelí-Marroquín et al, 2019 ). Furthermore, bimetallic silver–selenium nanoparticles were biosynthesized in a green manner using Orobanche aegyptiaca extract; these nanoparticles showed superior antimicrobial and antibiofilm activity against Staphylococcus aureus , P. aeruginosa , and Candida albicans ( Mostafa et al, 2023 ). Furthermore, pomegranate peel extract was used for the biosynthesis of bimetallic silver/zinc oxide nanoparticles that exhibited antimicrobial activity against E. coli , P. aeruginosa , B. subtilis , Staphylococcus aureus , Escherichia faecalis , Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus , and A. brasiliensis ( Hashem and El-Sayyad, 2023 ).…”

Section: Resultsmentioning

confidence: 99%

Green biosynthesis of bimetallic selenium–gold nanoparticles using Pluchea indica leaves and their biological applications

Aly Khalil,

Saied,

Mekky

et al. 2024

Front. Bioeng. Biotechnol.

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Increasing bacterial resistance and the negative impact of currently used antibacterial agents have produced the need for novel antibacterial agents and anticancer drugs. In this regard, nanotechnology could provide safer and more efficient therapeutic agents. The main methods for nanoparticle production are chemical and physical approaches that are often costly and environmentally unsafe. In the current study, Pluchea indica leaf extract was used for the biosynthesis of bimetallic selenium–gold nanoparticles (Se-Au BNPs) for the first time. Phytochemical examinations revealed that P. indica leaf extract includes 90.25 mg/g dry weight (DW) phenolics, 275.53 mg/g DW flavonoids, and 26.45 mg/g DW tannins. X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques were employed to characterize Se-Au BNPs. Based on UV-vis spectra, the absorbance of Se-Au BNPs peaked at 238 and 374 nm. In SEM imaging, Se-Au BNPs emerged as bright particles, and both Au and Se were uniformly distributed throughout the P. indica leaf extract. XRD analysis revealed that the average size of Se-Au BNPs was 45.97 nm. The Se-Au BNPs showed antibacterial properties against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis, with minimum inhibitory concentrations (MICs) of 31.25, 15.62, 31.25, and 3.9 μg/mL, respectively. Surprisingly, a cytotoxicity assay revealed that the IC50 value toward the Wi 38 normal cell line was 116.8 μg/mL, implying that all of the MICs described above could be used safely. More importantly, Se-Au BNPs have shown higher anticancer efficacy against human breast cancer cells (MCF7), with an IC50 value of 13.77 μg/mL. In conclusion, this paper is the first to provide data on the effective utilization of P. indica leaf extract in the biosynthesis of biologically active Se-Au BNPs.

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“…Recently, many researchers proposed Ag-based NPs synthesized using enzymes, microorganisms, or plant extracts [12][13][14]. In fact, there are many easy and green protocols for synthesizing AgNPs from natural constituents; however, these methods have limited applications as the NPs can only be only prepared in the solution phase [15,16].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and Antibiofilm Activity of Silver-Polypropylene Nanocomposites

Bellisario,

Santo,

Quadrini

et al. 2023

Antibiotics

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The development of biocompatible nanomaterials that interface with human skin and tissue is critical for advancing prosthetics and other therapeutic medical needs. In this perspective, the development of nanoparticles with cytotoxicity and antibiofilm properties and biocompatibility characteristics are important. Metallic silver (Ag) exhibits good biocompatibility, but it is often challenging to integrate it into a nanocomposite without compromising its antibiofilm properties for optimal applications. In this study, new polymer nanocomposites (PNCs) with ultra-low filling content (0.0023–0.046 wt%) of Ag nanoplates were manufactured and tested. The cytotoxicity and antibiofilm activity of different composites with polypropylene (PP) matrix were examined. At first, PNCs surface were analyzed by means of AFM (atomic force microscopy) with phase contrast evaluation and FTIR (Fourier-transform infrared spectroscopy) to study the Ag nanoplates distribution. Subsequently, the cytotoxicity and growth properties of biofilms were assessed by MTT assay protocol and detection of nitric oxide radicals. Antibacterial and antibiofilm activities were measured against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (K. pneumoniae). The PNCs with silver exhibited antibiofilm activity although they did not inhibit regular planktonic bacterial growth. Moreover, the PNCs were not cytotoxic to mammalian cells and did not induce significant immune response. These features reveal the potential of the PNCs developed in this study for usage in fabrication of prosthetics and other smart structures for biomedical applications.

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Promising antimicrobial and antibiofilm activities of Orobanche aegyptiaca extract-mediated bimetallic silver-selenium nanoparticles synthesis: Effect of UV-exposure, bacterial membrane leakage reaction mechanism, and kinetic study

Cited by 24 publications

References 96 publications

Watermelon Rind Mediated Biosynthesis of Bimetallic Selenium-Silver Nanoparticles: Characterization, Antimicrobial and Anticancer Activities

Watermelon Rind Mediated Biosynthesis of Bimetallic Selenium-Silver Nanoparticles: Characterization, Antimicrobial and Anticancer Activities

Green biosynthesis of bimetallic selenium–gold nanoparticles using Pluchea indica leaves and their biological applications

Cytotoxicity and Antibiofilm Activity of Silver-Polypropylene Nanocomposites

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