Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations

Lafraxo, Soufyane; Zouirech, Otmane; Barnossi, Azeddin El; Chelouati, Tarik; Chebaibi, Mohamed; Chebbac, Khalid; Nafidi, Hiba-Allah; Salamatullah, Ahmad Mohammad; Bourhia, Mohammed; Aboul‐Soud, Mourad A. M.; Bari, Amina

doi:10.1515/chem-2022-0332

Cited by 7 publications

(1 citation statement)

References 25 publications

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“…Moreover, the presence of monoterpenes and sesquiterpenes especially the major constituents (Elemol, β-Eudesmol and α-Eudesmol), and fatty acids, such as linoleic and oleic acids, may be one of the reasons for this activity. These components have been reported active against bacteria [68][69][70][71]. Compounds with high lipophilic structure (terpenes and fatty acids), can disrupt cell membrane integrity, leading to the loss of chemiosmotic control, and cell lysis [69].…”

Section: Uae and Soxhlet Extraction Comparisonmentioning

confidence: 99%

Green Extraction of Oleoresin from Pink Pepper Fruits: Effect of Experimental Conditions and Characterization

de Mello,

Hoscheid,

Raspe

et al. 2024

AppliedChem

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This work evaluated the green extraction of oleoresin from pink pepper fruits (ORPPF) using ultrasound-assisted extraction (UAE) and ethanol as a solvent. The effects of temperature, ultrasound power intensity, sample to solvent ratio and time on the global extraction yield (YGE) and phenolic compounds yield (YPC) were evaluated. The oleoresin samples were characterized and its antimicrobial activity determined, and the obtained results were compared to conventional extraction in Soxhlet. From the results it was found that the application of the highest levels of the independent variables favored the extraction process. The maximum values of YGE and YPC were 28.60 wt% and 6.37 mg GAE per g fruit, respectively, obtained at 60 °C, 100% of ultrasound power (165 W), 1:20 g mL−1 (sample:solvent) and 45 min. Under maximized conditions, the ORPPF obtained by UAE showed a content of phenolic compounds and antioxidant activity inferior to soxhlet–ethanol extraction. However, the time and solvent consumption were reduced. Oleic and linoleic acids predominated in the fatty acid composition of ORPPF, in addition to sesquiterpenes and gallic and syringic acids. The ORPPF presented weak antibacterial activity, with minimum inhibitory concentration ranging from 31.25 to 125 mg mL−1.

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Section: Uae and Soxhlet Extraction Comparisonmentioning

confidence: 99%

Green Extraction of Oleoresin from Pink Pepper Fruits: Effect of Experimental Conditions and Characterization

de Mello,

Hoscheid,

Raspe

et al. 2024

AppliedChem

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Phytochemical Diversity and Biological Effects of Juniperus Thurifera L., Essential Oil : A Comparative Study using In Vitro and In Silico Methods

Elhrech,

Aguerd,

El Omari

et al. 2024

Chemistry & Biodiversity

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This study aims to elucidate the phytochemical diversity and biological activities of J. thurifera essential oil (JTEO) through a comparative analysis of samples from two distinct regions: Tensift‐Al Haouz and Azilal, using both in vitro and in silico methods. Gas Chromatography‐Mass Spectrometry (GC‐MS) analysis revealed 21 components in the Tensift‐Al Haouz JTEO (99.99% of the oil) and 23 components in the Azilal JTEO (99.58% of the oil), with oxygenated monoterpenes being the predominant compounds in both. The biological activities were assessed in vitro. Antioxidant properties, evaluated using DPPH, FRAP, and ABTS assays, showed significant activity in both oils. Antibacterial activity was tested against two strains of Gram‐positive and two strains of Gram‐negative bacteria, with both oils demonstrating notable bacterial growth inhibition. Enzymatic assays assessed the antidiabetic (α‐amylase and α‐glucosidase), dermo‐protective (tyrosinase and elastase), and neuroprotective (AChE and BChE) activities. Both oils displayed substantial inhibitory effects across all tested activities, with variations attributed to their distinct chemical compositions. In silico analyses of six target enzymes confirmed significant binding affinities of the major compounds. Notably, 2,2’‐Thiobis(6‐tert‐butyl‐p‐cresol) exhibited strong binding affinities with AChE, BChE, α‐amylase, α‐glucosidase, tyrosinase, and elastase, with binding energies ranging from ‐10.0 to ‐6.2 kcal/mol.

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Chemical Profiling and Antioxidant, Antimicrobial, and Hemolytic Properties of Euphorbia calyptrata (l.) Essential oils: in Vitro and in Silico Analysis

El Kamari,

Zouirech,

Metouekel

et al. 2024

ChemistryOpen

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In this work, we sought to validate the use of Euphorbia calyptrata (L.), a Saharan and Mediterranean medicinal plant, in traditional pharmacopeia. GC‐MS/MS identified volatile compounds of potential therapeutic interest. Antioxidant tests were performed using β‐carotene decolorization, DPPH radical scavenging, FRAP, beta‐carotene bleaching, and TAC. The antimicrobial activity was evaluated on solid and liquid media for bacterial and fungal strains to determine the zone of inhibition and the minimum growth concentration (MIC) of the microbes tested. The hemolytic activity of these essential oils was assessed on red blood cells isolated from rat blood. Phytochemical characterization of the terpenic compounds by GC‐MS/MS revealed 31 compounds, with alpha‐Pinene dominating (35.96 %). The antioxidant power of the essential oils tested revealed an IC50 of 67.28 μg/mL (DPPH), EC50 of 80.25.08±1.42 μg/mL (FRAP), 94.83±2.11 μg/mL (beta carotene) and 985.07±0.70 μg/mL (TAC). Evaluating solid media‘s antibacterial and antifungal properties revealed a zone of inhibition between 10.28 mm and 25.80 mm and 31.48 and 34.21 mm, respectively. On liquid media, the MIC ranged from 10.27 μg/mL to 24.91 μg/mL for bacterial strains and from 9.32 μg/mL to 19.08 μg/mL for fungal strains. In molecular docking analysis, the compounds naphthalene, shogunal, and manol oxide showed the greatest activity against NADPH oxidase, with Glide G scores of −5.294, −5.218 and −5.161 kcal/mol, respectively. For antibacterial activity against E. coli beta‐ketoacyl‐[acyl carrier protein] synthase, the most potent molecules were cis‐Calamenene, alpha.‐Muurolene and Terpineol, with Glide G‐scores of −6.804, −6.424 and −6.313 kcal/mol, respectively. Hemolytic activity revealed a final inhibition of 9.42±0.33 % for a 100 μg/mL concentration. The essential oils tested have good antioxidant, antimicrobial, and hemolytic properties thanks to their rich phytochemical composition, and molecular docking analysis confirmed their biological potency.

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Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations

Cited by 7 publications

References 25 publications

Green Extraction of Oleoresin from Pink Pepper Fruits: Effect of Experimental Conditions and Characterization

Green Extraction of Oleoresin from Pink Pepper Fruits: Effect of Experimental Conditions and Characterization

Phytochemical Diversity and Biological Effects of Juniperus Thurifera L., Essential Oil : A Comparative Study using In Vitro and In Silico Methods

Chemical Profiling and Antioxidant, Antimicrobial, and Hemolytic Properties of Euphorbia calyptrata (l.) Essential oils: in Vitro and in Silico Analysis

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