“…In summary, accurate and rapid modification of nitrilases to increase their acid resistance, protease resistance, stability and ease of preparation and increase their stability and ease of preparation, according to agricultural and food application scenarios will greatly improve the potential application value of nitrilases in agriculture (FIG ). In addition, cofermentation by the addition of adding multiple enzymes such as feed enzymes , or other nitrile-converting enzymes also plays an important role in production activities. An accurate understanding of its catalytic mechanism and more comprehensive transformation, including further development of high-throughput screening methods and an understanding of the relationship between the two methods of nitrilase conversion of nitriles are expected to further expand the scale of nitrilase use in agricultural fields such as feed.…”