Promising genetic resources for resistance to wheat streak mosaic virus and the wheat curl mite in wheat-Thinopyrum partial amphiploids and their derivatives

Abstract

Wheat streak mosaic virus (WSMV), vectored by the wheat curl mite (WCM), Aceria tosichella Keifer, is one of the most destructive viral diseases of wheat found in many wheat producing areas of the world. Host resistance is the most effective method for controlling this disease and its vector. Symptomatological analysis and enzymelinked immunosorbent assay (ELISA) were used to characterize WSMV-resistance in wheat-alien partial amphiploid lines and their derivatives. The results showed that most of partial amphiploids derived from Thinopyrum ponticum and Th. intermedium were free of systemic symptoms with very low ELISA readings that were similar to that of the non-inoculated Chinese Spring control. While the partial amphiploid lines 693 and PWM706 were identified as new genetic resources of resistance to WSMV. The present study demonstrated that both symptomatological and ELISA methods efficiently assessed WSMV-resistance in the wheat-alien hybrids and systemic symptom incidence and ELISA absorbance readings were highly correlated (r 2 ¼ 0.8658-0.9323) over time following inoculation. The ELISA results also indicated that the virus did not buildup in the plant tissues of these virus-resistant partial amphiploids. Similar results were observed in chromosome translocation and substitution lines that have the gene Wsm1 conferring WSMV resistance. However, the lines containing the gene Wsm1 and all the partial amphiploid lines, except Agrotana, were susceptible to the WCM. One line derived from a cross of wheat and Agrotana, was effective in controlling the spread of WSMV and was highly resistant to the WCM. Another line and an accession of Triticum dicoccoides (Koern.) Schweinf. were highly susceptible to WSMV and WCM. Early disease development was delayed in a new hard red winter cultivar McClintock. The partial WSMV-resistance of McClintock was demonstrated by initially low ELISA readings, and a lower percentage of infected plants than other WSMV-susceptible wheat. The use of the identified promising sources of resistance to WSMV and the WCM in wheat breeding is discussed.

Detection of alien chromatin introgression from <i>Thinopyrum</i> into wheat using S genomic DNA as a probe – A landmark approach for <i>Thinopyrum</i> genome research

“…Recently, it was reported that the wheat-Th. intermedium partial amphiploid MT-2 was resistant not only to WSMV and WCM , but also to eyespot and Cephalosporium stripe (Cox et al, 2002;Li et al, 2003b).…”

“…Resistance to the WCM in lines derived from Agrotana, which is immune to WSMV, is controlled by two Th. ponticum J S chromosomes (Chen et al, 1999a;Li et al, 2003b). Recently, the resistance to common root rot (Cochliobolus sativus [Ito and Kurib] Drechs.…”

Virus Resistance in Cereals: Sources of Resistance, Genetics and Breeding

“…In addition, it was shown, like for other diseases, that Th. intermedium is a valuable source for introducing mite and WSMV resistance into wheat (Friebe et al., 1996; Cox et al., 2002; Li et al., 2004) and molecular markers specific for these introgressions have been developed (Chen et al., 1998a,b, 1999, 2003). In addition, two temperature‐sensitive resistances to WSMV have been recently described by Seifers et al.…”

What’s “cool” on eriophyoid mites?

“…Great attention has been also paid to the discovery and breeding of WSMV-and WCM-resistant wheat varieties as a promising methods for controlling this mite-vectored disease (e.g. Chen et al 2003;Malik et al 2003;Thomas et al 2004;Harvey et al 2005;Li et al 2005Li et al , 2007. Aceria tosichella has been identified as a vector of three other plant disease viruses, namely Brome Streak Mosaic Virus (Stephan et al 2008), Triticum Mosaic Virus (Seifers et al 2009), and High Plain Disease (Mahmood et al 1998;Seifers et al 2002;Skare et al 2003Skare et al , 2006.…”