Promising Transcription Factors for Salt and Drought Tolerance in Plants

Goel, Parul; Bhuria, Monika; Sinha, Ragini; Sharma, Tilak Raj; Singh, Anil Kumar

doi:10.1007/978-981-15-0690-1_2

Cited by 11 publications

(7 citation statements)

References 306 publications

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“…According to the current results, at 6 h of PEG-induced drought stress, leaf tissues start to sense the stress through the early perception of water deficiency leading to activation of the stress-responsive genes and stimulation of stomatal closure to reduce water loss 48 . The highest expressed gene in leaves at 6 h of drought stress was ATP-dependent Clp proteases (eightfolds higher than control).…”

Section: Discussionmentioning

confidence: 71%

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Arisha

Ahmad

Tang

et al. 2020

Sci Rep

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Purple-fleshed sweet potato (PFSP) is an important food crop, as it is a rich source of nutrients and anthocyanin pigments. Drought has become a major threat to sustainable sweetpotato production, resulting in huge yield losses. Therefore, the present study was conducted to identify drought stressresponsive genes using next-generation (NGS) and third-generation sequencing (TGS) techniques. Five cDNA libraries were constructed from seedling leaf segments treated with a 30% solution of polyethylene glycol (PEG-6000) for 0, 1, 6, 12, and 48 h for second-generation sequencing. Leaf samples taken from upper third of sweet potato seedlings after 1, 6, 12, and 48 h of drought stress were used for the construction of cDNA libraries for third-generation sequencing; however, leaf samples from untreated plants were collected as controls. A total of 184,259,679 clean reads were obtained using second and third-generation sequencing and then assembled into 17,508 unigenes with an average length of 1,783 base pairs. Out of 17,508 unigenes, 642 (3.6%) unigenes failed to hit any homologs in any databases, which might be considered novel genes. A total of 2, 920, 1578, and 2,418 up-regulated unigenes and 3,834, 2,131, and 3,337 down-regulated unigenes from 1 h, 6 h, 12 h, and 48 h library were identified, respectively in drought stress versus control. In addition, after 6, 12, and 48 h of drought stress, 540 up-regulated unigenes, 486 down-regulated unigenes and 414 significantly differentially expressed unigenes were detected. It was found that several gene families including Basic Helix-loop-helix (bHLH), basic leucine zipper (bZIP), Cystein2/Histidine2 (C2H2), C3H, Ethylene-responsive transcription factor (ERF), Homo domain-leucine zipper (HD-ZIP), MYB, NAC (NAM, ATAF1/2, and CUC2), Thiol specific antioxidant and WRKY showed responses to drought stress. In total, 17,472 simple sequence repeats and 510,617 single nucleotide polymorphisms were identified based on transcriptome sequencing of the PFSP. About 96.55% of the obtained sequences are not available online in sweet potato genomics resources. Therefore, it will enrich annotated sweet potato gene sequences and enhance understanding of the mechanisms of drought tolerance through genetic manipulation. Moreover, it represents a sequence resource for genetic and genomic studies of sweet potato. Purple-fleshed sweet potato (PFSP) (Ipomoea batatas (L.) Lam.) is a type of sweet potato, which has purple skin and flesh color of the storage roots due to the large accumulation of anthocyanin pigments 1. It is a root crop widely grown in some Asian and African countries (i.e. China, India, and Kenya). Sweet potato is regarded as the seventh most important food crop. It is among the crops which generate large amounts of food per unit area per unit time 2. Furthermore, it is considered to be a cheap source of energy, carbohydrates, vitamin, potassium, iron, fiber, and protein 3 .

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Section: Discussionmentioning

confidence: 71%

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Arisha

Ahmad

Tang

et al. 2020

Sci Rep

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“…TFs are considered early activated genes that are targeted by phosphatases and protein kinases [ 41 ]. Most of the TFs that play important roles in abiotic stress tolerance in plants fall into major TF families (AP2/ERF, NAC, MYB/MYC, WRKY, bZIP, bHLH, and ZFP) [ 42 ]. A previous study in Sophora alopecuroides functionally annotated a total of 11,936 TF genes from 82 TF families under salt, alkali, and drought stress [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

et al. 2021

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Background Sophora tonkinensis Gagnep is a traditional Chinese medical plant that is mainly cultivated in southern China. Drought stress is one of the major abiotic stresses that negatively impacts S. tonkinensis growth. However, the molecular mechanisms governing the responses to drought stress in S. tonkinensis at the transcriptional and posttranscriptional levels are not well understood. Results To identify genes and miRNAs involved in drought stress responses in S. tonkinensis, both mRNA and small RNA sequencing was performed in root samples under control, mild drought, and severe drought conditions. mRNA sequencing revealed 66,476 unigenes, and the differentially expressed unigenes (DEGs) were associated with several key pathways, including phenylpropanoid biosynthesis, sugar metabolism, and quinolizidine alkaloid biosynthesis pathways. A total of 10 and 30 transcription factors (TFs) were identified among the DEGs under mild and severe drought stress, respectively. Moreover, small RNA sequencing revealed a total of 368 miRNAs, including 255 known miRNAs and 113 novel miRNAs. The differentially expressed miRNAs and their target genes were involved in the regulation of plant hormone signal transduction, the spliceosome, and ribosomes. Analysis of the regulatory network involved in the response to drought stress revealed 37 differentially expressed miRNA-mRNA pairs. Conclusion This is the first study to simultaneously profile the expression patterns of mRNAs and miRNAs on a genome-wide scale to elucidate the molecular mechanisms of the drought stress responses of S. tonkinensis. Our results suggest that S. tonkinensis implements diverse mechanisms to modulate its responses to drought stress.

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“…Multi‐omics techniques that assess many aspects of TF activity are suitable for studying the interplay between cell signaling and gene regulation, with TFs serving as the perfect bridge between the two disciplines (Weidemüller et al, 2021). Under salinity stress, the major TFs which play a critical role in plant survival are NAC , WRKY , bZIP , DREB , AP2 , and C2H2 Zn‐finger families (Goel et al, 2019; Gupta & Huang, 2014). Regulatory TFs attach to the specific regions of promoters of stress‐responsive genes and hence alter their expression to mitigate the stress injury.…”

Section: Rice Tfs Crosstalks With Cell Signaling and Gene Regulation ...mentioning

confidence: 99%

Seedling‐stage salinity tolerance in rice: Decoding the role of transcription factors

Tiwari

Nutan

Deshmukh

et al. 2022

Physiologia Plantarum

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Rice is an important staple food crop that feeds over half of the human population, particularly in developing countries. Increasing salinity is a major challenge for continuing rice production. Though rice is affected by salinity at all the developmental stages, it is most sensitive at the early seedling stage. The yield thus depends on how many seedlings can withstand saline water at the stage of transplantation, especially in coastal farms. The rapid development of “omics” approaches has assisted researchers in identifying biological molecules that are responsive to salt stress. Several salinity‐responsive quantitative trait loci (QTL) contributing to salinity tolerance have been identified and validated, making it essential to narrow down the search for the key genes within QTLs. Owing to the impressive progress of molecular tools, it is now clear that the response of plants toward salinity is highly complex, involving multiple genes, with a specific role assigned to the repertoire of transcription factors (TF). Targeting the TFs for improving salinity tolerance can have an inbuilt advantage of influencing multiple downstream genes, which in turn can contribute toward tolerance to multiple stresses. This is the first comparative study for TF‐driven salinity tolerance in contrasting rice cultivars at the seedling stage that shows how tolerant genotypes behave differently than sensitive ones in terms of stress tolerance. Understanding the complexity of salt‐responsive TF networks at the seedling stage will be helpful to alleviate crop resilience and prevent crop damage at an early growth stage in rice.

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Promising Transcription Factors for Salt and Drought Tolerance in Plants

Cited by 11 publications

References 306 publications

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

Seedling‐stage salinity tolerance in rice: Decoding the role of transcription factors

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