Promoção de crescimento vegetal por &lt;i&gt;Bacillus&lt;/i&gt; sp. RZ2MS9: dos genes ao campo

Batista, Bruna Durante

doi:10.11606/t.11.2017.tde-15082017-170543

Cited by 6 publications

(6 citation statements)

References 87 publications

(114 reference statements)

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“…It was found several genes for parts of the iron-and siderophore-uptake systems. In field experiments, maize inoculated with RZ2MS9 demonstrate an increase of 16 bags per hectare, compared with the control non-inoculated, using a lower nitrogen application than usual (Batista, 2017). These previous results demonstrate the potential of this strain to be used as bio-fertilizer.…”

Section: Introductionmentioning

confidence: 52%

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Almeida¹

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Molecular mechanisms involved in the bacterial talking and maize growth promotion With the increase of agricultural production, there is an improvement in the use of mineral fertilizers, which may cause different environmental problems, besides the soil salinization. A possible alternative for reducing the application of these products is the use of plant growth-promoting bacteria (PGPB), that can be used alone or in co-inoculation, resulting in an alternative environmentally and economically feasible. Better results can be obtained if the interaction among bacteria-bacteria and bacteria-plant be elucidated, and strategy developed to optimize these interactions. Thus, the plant growth-promoting Bacillus sp. RZ2MS9, previous described as a potential PGPB in maize and soybean, was GFP-tagged and monitored alone and co-inoculated with Azospirillum brasilense (Ab-v5::pWM1013) during maize colonization. The interaction of tagged strains in maize were monitored by fluorescent microscopy (FM) and quantitative PCR (qPCR), demonstrating an endophytic behavior of Bacillus sp. RZ2MS9. Although the non-detection of Ab-v5::pWM1013, the co-inoculation resulted in the best increase in root and shoot dried weight, root volume and in root diameter, showing that inoculation with more than one strain can be a good choice to development of bio-fertilizers. One important system to bacterial interaction is the quorum sensing (QS). The QS is an important cell-cell communication system that allows bacterial cells to recognize their own population and modulate their gene expression. This, system is also involved in the interspecific communication, including other bacterial species and plants. In the other hand, enzymes able to detect and degrade these molecules evolved, the called quorum quenching (QQ) system, that has been evolved in some bacteria as competitive advantage for niches colonization. The aiiA gene, was one of the first gene related with the QQ in Bacillus. The aiiA was found in Bacillus sp. RZ2MS9 genome. Through construction of a new QQ biosensor, Agrobacterium tumefaciens At11006, and validated by A. tumefaciens NTL4, the ability of RZ2MS9 to degrade QS molecules was confirmed. The knockout of aiiA gene was performed using the CRISPR-Cas9 system, confirming this gene function. By these results, the influence of QQ system of Bacillus sp. RZ2MS9 during maize colonization and RZ2MS9-A. brasilense-maize can be better investigated, opens the possibility to better understand the role of QQ system in the interaction among PGPB and plants.

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Section: Introductionmentioning

confidence: 52%

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Almeida¹

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“…According to LannaFilho et al (2010), the rapid development of the seedling conditions it to reach the adult stage faster, remaining less time in the field, which favors the escape against pathogens present in the soil and in the external environment, it can also promote greater resistance to adverse abiotic conditions by being nutritionally balanced.The inoculation of B. subtilis Bs10 provided an increase of productivity at three fulfilled experiments, the increase of the trial had a variation of 186 to 23.3 a 1398 kg ha -1 . That raise is a result of the benefits and action mechanisms by which the inoculation with B. subtilis Bs10 proportionate throughout the plant's growth Batista (2017). verified a development in soybean productivity which received the inoculation of B. japonicum SEMIA 5080 and of B. subtilis RZ2MS9, corresponding to an increase of 35 and 11 sacks per hectare more than the control.…”

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confidence: 75%

Efficiency of Bacillus subtilis Bs10 as a plant growth promoting inoculant in soybean crop under field conditions

Chagas

Martins

et al. 2021

RSD

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The growth of the Brazilian market for biological defensive follows a worldwide trend of reducing the use of pesticides in crops, due to international and societal demands for a more sustainable agriculture. The objective of the present work was to evaluate the efficiency of Bacillus subtilis Bs10 as an inoculant for the soybean crop, through the promotion of plant growth and productive performance in the field. Three field experiments were conduct in the municipalities of Porto Nacional and Formoso do Araguaia, Tocantins, Brazil. The treatments used in the experiments were five doses of the B. subtilis Bs10-based product (0, 100, 200, 300 and 400 mL 50 kg of seeds-1), plus one treatment with a commercial B. subtilis-based product. The inoculation of B. subtilis Bs10 provided increases in biomass, agronomic characteristics, plant population and productivity in soybeans under field conditions. There were positive results starting at a dose of 200 mL, with gains in productivity ranging from 28 to 41% in relation to the absolute control treatment, without inoculation of B. subtilis, and from 11 to 42% in relation to the treatment with commercial product. The bio formulated B. subtilis Bs10 should be recommend as a plant growth promoting inoculant in soybean.

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“…Bacteria of the Firmicutes phylum are commonly found in the soil (Cardoso & Andreote, 2016), and play important functions of agricultural interest. The predominance of the Bacillus genus was observed by Bomfim (2011), species of this genus are widely reported as plant growth promoters as they are capable of synthesizing phytohormones such as auxins (Batista, 2017) that act in plant development and growth. Another important reported genus is Lactoccocus, bacteria of this genus produce antimicrobial substances, including lactic acid, which contribute to the suppression of pathogens (Bomfim, 2011;Tanya & Leiva, 2019).…”

Section: Composition Of Emmentioning

confidence: 94%

Use of efficient microorganisms in agriculture

Avila

Gabardo

Clock

et al. 2021

RSD

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The interest in the use of microorganisms in agricultural practices has increased significantly in recent years, both in the promotion of plant growth and in the biological control of plant pests and diseases. This literature review work aimed to address information on the use of isolation, multiplication, use and storage methodologies for efficient microorganisms (Effective Microorganisms, EM) in agriculture. These microorganisms have important functions for their hosts, as they have symbiotic interactions with them, and are capable of protecting plants from attack by insects, diseases and herbivorous mammals through the production of toxins. The use of EM in agriculture aims to accelerate the natural composition of organic matter and promote the balance of microbial flora contributing to plant development. EMs are potential substitutes for chemical products, and can thus favor the preservation of the environment. They are collected from fertile forest soils through simple and inexpensive methodologies, consisting of a tool with potential to be used both by family farmers and on a small and large scale. They can be used in different ways, the main ones being in soil, plant, water and animals. The use of EM is an accessible and low-cost technique, in addition to being easy to prepare within the property itself, contributing to the sustainability of agricultural systems.

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Promoção de crescimento vegetal por <i>Bacillus</i> sp. RZ2MS9: dos genes ao campo

Cited by 6 publications

References 87 publications

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Efficiency of Bacillus subtilis Bs10 as a plant growth promoting inoculant in soybean crop under field conditions

Use of efficient microorganisms in agriculture

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