Promoter activity and regulation of the Pou5f1 homolog from a teleost, Nile tilapia

Wei, Jing; Huang, Xiaohuan; Fan, Zhenhua; Yang, Zhuo; Ding, Fan; Zhou, Lei; Wang, Deshou

doi:10.1016/j.gene.2017.11.036

Cited by 6 publications

(1 citation statement)

References 38 publications

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“…MZT activation. Studies of the expression patterns of oct4 in medaka (Oryzias latipes) [95][96][97][98], Nile tilapia (Oreochromis niloticus) [99,100], and zebrafish [101][102][103][104][105] have documented its importance for normal fish embryonic development and survival. In zebrafish, maternally inherited oct4, sox2 (soxb1), and nanog transcripts are responsible for activating zygotic expression and initiating the clearance of maternal mRNA through activation of microRNA, hence they are considered fundamental for successful MZT [103].…”

Section: Molecular Ontogeny and Embryonic Developmentmentioning

confidence: 99%

Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel

et al. 2020

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Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergic inhibition of the neuroendocrine system, preventing gonadotropin secretion and gonadal development. The present study used either CPE or SPE to induce vitellogenesis in female European eel and compared impacts on egg quality and offspring developmental competence with emphasis on the maternal-to-zygotic transition (MZT). Females treated with SPE produced significantly higher proportions of floating eggs with fewer cleavage abnormalities and higher embryonic survival. These findings related successful embryogenesis to higher abundance of mRNA transcripts of genes involved in cell adhesion, activation of MZT, and immune response (dcbld1, epcam, oct4, igm) throughout embryonic development. The abundance of mRNA transcripts of cldnd, foxr1, cea, ccna1, ccnb1, ccnb2, zar1, oct4, and npm2 was relatively stable during the first eight hours, followed by a drop during MZT and low levels thereafter, indicating transfer and subsequent clearance of maternal mRNA. mRNA abundance of zar1, epcam, and dicer1 was associated with cleavage abnormalities, while mRNA abundance of zar1, sox2, foxr1, cldnd, phb2, neurod4, and neurog1 (before MZT) was associated with subsequent embryonic survival. In a second pattern, low initial mRNA abundance with an increase during MZT and higher levels persisting thereafter indicating the activation of zygotic transcription. mRNA abundance of ccna1, npm2, oct4, neurod4, and neurog1 during later embryonic development was associated with hatch success. A deviating pattern was observed for dcbld1, which mRNA levels followed the maternal-effect gene pattern but only for embryos from SPE treated females. Together, the differences in offspring production and performance reported in this study show that PE composition impacts egg quality and embryogenesis and in particular, the transition from initial maternal transcripts to zygotic transcription.

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