Promoter Elements of the Rat Choline Acetyltransferase Gene Allowing Nerve Growth Factor Inducibility in Transfected Primary Cultured Cells

Bejanin, Stéphane; Habert, Estelle; Berrard, Sylvie; Edwards, Jean-Baptiste Dumas Milne; Loeffler, Jean‐Philippe; Mallet, Jacques

doi:10.1111/j.1471-4159.1992.tb11383.x

Cited by 50 publications

(22 citation statements)

References 19 publications

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“…The sequences of the corresponding exons, numbered 1 and 2, were identical with those previously reported (Hersh et al, 1989;Toussaint et al, 1992). A 1.9-kb Hind111 rat genomic fragment (Bejanin et al, 1992) ern blots of human cosmid DNA at moderate stringency. The probe labeled a 1.7-kbp ApaI-XhoI fragment, which was located from 8.1 to 6.4 kbp upstream of exon 1, and included the human homolog of rodent exon R. A dotplot comparison of the sequence of the ApaI-XhoI fragment with the published rat genomic sequence (Ibaiiez et al, 1991;Bejanin et al, 1992) is presented on Figure 2B.…”

Section: Localization Of 5' Exonsmentioning

confidence: 90%

Human choline acetyltransferase gene: Localization of alternative first exons

Chireux

Thai

Weber

1995

J of Neuroscience Research

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Two overlapping cosmids containing the 5' end of human choline acetyltransferase (ChAT) gene have been cloned. Using heterologous probes, we localized two alternative first exons homologous to rodent ChAT exons R and M (Misawa et al.: J Biol Chem 267:20392-20399, 1992). The sequence of rodent exon N was not conserved in the human gene. Northern blot analysis of mRNA purified from the human neuroepithelioma cell lines LA-N2 and MC-I-XC revealed that both exons R and M were transcribed in mRNA species of 6.0 and 2.5 kb. Only the 6-kb species was detected with both R- and M-specific probes in the neuroepithelioma cell line CHP126. Reverse transcription-polymerase chain reaction (RT-PCR) analysis suggested that the major mRNA species in MC-I-XC and CHP126 cells contained the proximal part of exon M spliced to exon 1, which contains the alternative ACG initiation codon. RT-PCR also allowed the characterization of a mRNA species containing exon R spliced to exon 1, but no species containing both exon R and the distal part of exon M could be detected. RT-PCR was also used to evidence an alternative exon (tentatively numbered exon 8) in the coding sequence.

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Section: Localization Of 5' Exonsmentioning

confidence: 90%

Human choline acetyltransferase gene: Localization of alternative first exons

Chireux

Thai

Weber

1995

J of Neuroscience Research

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“…These mRNA isoforms appear to be generated from either a transcriptional start site upstream of the R exon, which may also be used for ChAT, or from sites within the VAChT exon (Bejanin et al, 1992;Misawa et al, 1992;Kengaku et al, 1993;Alfonso et al, 1994;Cervini et al, 1995), Fig. 1.…”

Section: Genomic Structure and Transcription Of The Cholinergic Gene mentioning

confidence: 98%

Regulation of the cholinergic gene locus by the repressor element-1 silencing transcription factor/neuron restrictive silencer factor (REST/NRSF)

Shimojo

Hersh

2004

Life Sciences

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“…A variety of neurotrophic factors, including nerve growth factors (NGF), are known to participate in the regulation of ChAT mRNA expression (Bejanin et al, 1992;Cavicchioli et al, 1991;Cervini et al, 1994;Misawa et al, 1993;Nawa et al, 1991;Pedersen et al, 1995). By using an antiserum against VAChT, Takei et al (1997a) reported that NGF and brain-derived neurotrophic factor (BDNF) increased the number of immunoreactive neurons and enhanced immunoreactivity in cultured rat embryonic septal cells.…”

Section: Introductionmentioning

confidence: 98%

Nerve growth factor increases the synthesis and release of acetylcholine and the expression of vesicular acetylcholine transporter in primary cultured rat embryonic septal cells

1999

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Acetylcholine (ACh) is synthesized by choline acetyltransferase (ChAT) in the cytoplasm of cholinergic nerve terminals and transported into synaptic vesicles by vesicular ACh transporter (VAChT). The genes encoding ChAT and VAChT are colocalized within the genome, and their products are known to be coregulated by various neurotrophic factors. In the present study, nerve growth factor (NGF; 100 ng/ml) was shown to enhance expression of VAChT and ChAT mRNA in primary cultured rat embryonic septal cells. By using a radioimmunoassay, we also found that NGF increased both neuronal content and spontaneous release of ACh, which were first detected on day 2 of culture and time-dependently increased up to day 10. Stimulated release of ACh elicited by high K+ (50 mM KCl) was also significantly greater in NGF-treated cells than in control cells. NGF enhanced immunoreactivity to antiserum against VAChT, indicating that the augmented responses were due to, at least in part, increased expression of VAChT protein. In contrast, the numbers of immunocytochemically positive cells were unaffected. Thus, NGF appears to augment ACh synthesis, its transport into synaptic vesicles, and its subsequent release. The data also suggest that NGF facilitates growth and development of cholinergic neurons, but not their survival.

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Promoter Elements of the Rat Choline Acetyltransferase Gene Allowing Nerve Growth Factor Inducibility in Transfected Primary Cultured Cells

Cited by 50 publications

References 19 publications

Human choline acetyltransferase gene: Localization of alternative first exons

Human choline acetyltransferase gene: Localization of alternative first exons

Regulation of the cholinergic gene locus by the repressor element-1 silencing transcription factor/neuron restrictive silencer factor (REST/NRSF)

Nerve growth factor increases the synthesis and release of acetylcholine and the expression of vesicular acetylcholine transporter in primary cultured rat embryonic septal cells

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