Promoter hypermethylation of <i><scp>SOX</scp>11</i> correlates with adverse clinicopathological features of human prostate cancer

Pugongchai, Apiwat; Bychkov, Andrey; Sampatanukul, Pichet

doi:10.1111/iep.12257

Cited by 13 publications

(10 citation statements)

References 33 publications

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“…At the same time, enormous research is being carried out for the identification of novel and clinically efficient blood-based and urinary cancer biomarkers [ 56 , 57 , 58 ]. In this respect, a number of novel assay concepts have been reported that detect DNA hypermethylation, a characteristic trait of prostate cancer associated with the epigenetic silencing of key genes such as GST-Pi and SOX11 [ 59 , 60 , 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

Mavrikou

Moschopoulou

Zafeirakis³

et al. 2018

Sensors

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Prostate-specific antigen (PSA) is the established routine screening tool for the detection of early-stage prostate cancer. Given the laboratory-centric nature of the process, the development of a portable, ultra rapid high-throughput system for PSA screening is highly desirable. In this study, an advancedpoint-of-care system for PSA detection in human serum was developed based on a cellular biosensor where the cell membrane was modified by electroinserting a specific antibody against PSA. Thirty nine human serum samples were used for validation of this biosensory system for PSA detection. Samples were analyzed in parallel with a standard immunoradiometric assay (IRMA) and an established electrochemical immunoassay was used for comparison purposes. They were classified in three different PSA concentration ranges (0, <4 and ≥4 ng/mL). Cells membrane-engineered with 0.25 μg/mL anti-PSA antibody demonstrated a statistically lower response against the upper (≥4 ng/mL) PSA concentration range. In addition, the cell-based biosensor performed better than the immunosensor in terms of sensitivity and resolution against positive samples containing <4 ng/mL PSA. In spite of its preliminary, proof-of-concept stage of development, the cell-based biosensor could be used as aninitiative for the development of a fast, low-cost, and high-throughput POC screening system for PSA.

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Section: Discussionmentioning

confidence: 99%

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

Mavrikou

Moschopoulou

Zafeirakis³

et al. 2018

Sensors

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“…In addition, the loss of endogenous SOX5 expression attenuates the invasion and metastasis of gastric cancer cells, while overexpression of SOX5 signi cantly reverses these events [23]. Furthermore, other studies have pointed out that member proteins of SOX family detected in human cancer tissues, were served as potential molecular markers [24]. It is proved that SOX5 is up-regulated in various forms of cancer and is associated with poor prognosis of patients.…”

Section: Discussionmentioning

confidence: 99%

MiR-219-5p is Involved in the Proliferation, Migration and Invasion of Oral Cancer Through SOX5 Regulation

Zhou

He²,

Dong

et al. 2021

Preprint

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Purpose Oral cancer has the characteristics of rapid progression, wide invasion and poor prognosis, which induces higher mortality in the patients. At present, there are about 300 thousand new cases of oral cell carcinoma worldwide. Particularly, the incidence rate of oral cancer in China is relatively high. Therefore, it urgently needs to understand the pathogenesis of oral cancer and molecular mechanisms underlying. Abnormal regulation of miR-219-5p is present in various types of cancer. However, the relationship between miR-219-5p and its targets in oral cancer has not been well evaluated. Methods Western blotting and Quantitative RT-PCR were used to detect the expression of SOX5 in oral cancer tissues.Migration ,cell proliferation,and invasion were detected using CCK8 assay,Conlony formation assay and Transwell assays .The interaction between SOX5 and miR-219-5p and oral cancer was confirmed using dual-luciferase reporter assays.Result This study aims to investigate the possible roles of miR-219-5p and its potential target gene, SOX5, in the progress of oral cancer. Our data showed that the high miR-219-5p and low SOX5 expression levels were associated with improved survival rates in patients. miR-219-5p level was negatively correlated with the expression of SOX5. Genetic analysis and luciferase assay revealed that the miR-291-5p regulated SOX5 expression by targeting the 3'-UTR region of SOX5 mRNA. Functionally, we confirmed that miR-219-5p mimics inhibited SOX5 expression and suppressed the proliferation, colony formation ability, invasion and migration of oral cancer cells, SCC4 and SCC9. In contrast, inhibition of miR-219-5p increased SOX5 levels and promoted the vitality and mobility of oral cancer cells. Furthermore, special siRNA targeting SOX5 partially neutralized the effects of miR-219-5p inhibitor. Conclusions This study demonstrates that miR-219-5p may inhibit the proliferation, migration and invasion of oral cancer by targeting the expression of SOX5, which provided novel candidates for clinic prognosis and/or therapy.

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“…Aberrant DNA methylation of SOX11 has been found in cancers such as prostate, gastric, ovarian, and chronic lymphocytic leukemia [13,[18][19][20][21]. In high-grade epithelial ovarian cancer, SOX11 was a tumor suppressor that associated with improved survival [13].…”

Section: Discussionmentioning

confidence: 99%

“…In high-grade epithelial ovarian cancer, SOX11 was a tumor suppressor that associated with improved survival [13]. In prostate cancer, SOX11 hypermethylation associated with adverse clinicopathologic characteristics, including higher PSA levels, high Gleason scores, and perineural invasion [21]. In gastric cancer, SOX11 methylation associated with Helicobacter pylori infection and related to tumor invasion, Borrmann classification, and tumor differentiation status [19,20].…”

Section: Discussionmentioning

confidence: 99%

SOX11 hypermethylation as a tumor biomarker in endometrial cancer

et al. 2019

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We previously reported that SOX4 is overexpressed in endometrial cancer and that it partially contributes to hypermethylation of miR-129-2 and miR-203. The current study seeks to identify methylation and expression levels of the SOX gene family in endometrial carcinomas. Methylation levels of the 16 SOX gene family members were measured by combining bisulfite restriction analysis (COBRA), MassARRAY, and pyrosequencing assays of cell lines and endometrial cancer samples. Gene expression was determined by RT-qPCR. The methylation level of the SOX11 locus

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Promoter hypermethylation of SOX11 correlates with adverse clinicopathological features of human prostate cancer

Cited by 13 publications

References 33 publications

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

MiR-219-5p is Involved in the Proliferation, Migration and Invasion of Oral Cancer Through SOX5 Regulation

SOX11 hypermethylation as a tumor biomarker in endometrial cancer

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