Promoter methylation of E-cadherin gene in gastric mucosa associated with Helicobacter pylori infection and in gastric cancer

Chan, Annie On‐On; Lam, Shiu Kum; Wong, Benjamin C Y; Wong, Wai Man; Yuen, Man Fung; Yeung, Y; Hui, W. M.; Rashid, Asif; Kwong, Yok Lam

doi:10.1136/gut.52.4.502

Cited by 270 publications

(289 citation statements)

References 22 publications

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“…In this study, we investigated DNA methylation of 12 tumor-related genes, of which only 2, CDH1 and RAR-beta, have been shown to be involved in tumor invasion or metastasis. 56,57 In the present study, DNA methylation of CDH1 and RARbeta was correlated with advanced T grade. However, DNA methylation of each of the 12 genes was not associated with tumor stage but occurred frequently in the high-methylation group, and therefore, the association between high-methylation group and tumor stage is not a secondary effect of the loss of specific biologic functions.…”

Section: Discussionmentioning

confidence: 84%

“…59 In GC, DNA methylation of CDH1 has been reported to be correlated with H. pylori infection. 56 Conversely, it has been reported that in nonneoplastic gastric mucosa, H. pylori infection is not correlated with the number of methylated genes. 60 In the present study, no correlation was found between methylation of any of 12 genes and presence of H. pylori infection in corresponding nonneoplastic gastric mucosa and normal gastric mucosa.…”

Section: Discussionmentioning

confidence: 99%

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Accumulation of DNA methylation is associated with tumor stage in gastric cancer

Oue

Mitani

Motoshita

et al. 2006

Cancer

129

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We present a methodological framework for estimating the degree of mixing between successive miscible fluids pumped along a near‐horizontal pipe. Either or both of the fluids can be non‐Newtonian, of Herschel–Bulkley type. Overall it is considered that the objective is to minimise mixing. In laminar regimes our estimates are based on front velocity of the leading displacement front. In turbulent regimes the spreading mechanism is dispersion. In addition to the estimates of mixing volumes/lengths, we also predict a minimal flow rate necessary in order to achieve a successful displacement of the residual fluid. © 2013 Canadian Society for Chemical Engineering

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Accumulation of DNA methylation is associated with tumor stage in gastric cancer

Oue

Mitani

Motoshita

et al. 2006

Cancer

129

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“…This result is consistent with those of previous studies that have demonstrated the close association of HP infection with aberrant CpG island hypermethylation. [25][26][27] We found that HP infection was a confounding factor in the comparison of methylation between CG and GCN and the analysis restricted to HP-positive cases did not show any difference in the number of methylated genes between CG and GCN. This was in contrast to the previous study of Waki et al, 28 who demonstrated higher number of methylated CpG island loci in GCN than in CG using MSP.…”

Section: Methylation In Gc Was Revealed For the First Time In Thismentioning

confidence: 88%

DNA methylation profiles of gastric carcinoma characterized by quantitative DNA methylation analysis

Kang

Lee

Cho

et al. 2008

Laboratory Investigation

153

107

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Transcriptional silencing by CpG island hypermethylation is a potential mechanism for the inactivation of tumor-related genes. Virtually, all types of human cancers show CpG island hypermethylation, and gastric carcinoma (GC) is one of the tumors with a high frequency of aberrant CpG island hypermethylation. In this study, we prescreened DNA methylation of 170 CpG island loci in a training set of 8 paired GC and GC-associated non-neoplastic mucosae (GCN) using MethyLight technology and selected 27 DNA methylation markers showing higher methylation frequency or level in GC than in GCN. These markers were then analyzed in a tester set of 25 paired GC and GCN and 27 chronic gastritis (CG) from non-cancer patients to generate their DNA methylation profiles. We identified 17 novel methylation markers in GC, including SFRP4, SEZ6L, TWIST1, BCL2, KL, TERT, SCGB3A1, IGF2, GRIN2B, SFRP5, DLEC1, HOXA1, CYP1B1, SMAD9, MT1G, NR3C1, and HOXA10. Of the 27 selected CpG island loci, 23 were methylated in GC, GCN, and CG and the remainder four loci (DLEC1, CHFR, CYP1B1, and NR3C1) were only methylated in GC. We found that the number of methylated loci was significantly higher in GC than in GCN or CG and that Helicobacter pylori infection was strongly associated with aberrant CpG island hypermethylation in CG. Hypermethylation was more prevalent in Epstein-Barr virus (EBV)-positive GC than in EBV-negative GC and in diffuse-type GC than in intestinal-type GC. Through our large-scale screening of 170 CpG island loci, we found 17 new DNA methylation markers of GC, which may serve as useful markers that may identify a distinct subset of GC.

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“…Whether the methylation differences at CpGs 3, 7 and 8 contribute to TB predisposition or are merely the result of infection with M. tuberculosis and treatment of TB is unknown. Chan et al [34] showed that E-cad promoter methylation is initiated by Helicobacter pylori infection, leading to gastric carcinogenesis. Kovalenko et al [35] showed that treatment with the anti-tuberculosis drug pyrazinamide leads to methylation alterations of LINE-1 (long interspersed nucleotide elements), GSTP (glutathione-Stransferase) and p16(INK4A) genes in rat liver.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D receptor gene methylation is associated with ethnicity, tuberculosis, and TaqI polymorphism

et al. 2011

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The Vitamin D Receptor (VDR) gene encodes a transcription factor which, on activation by vitamin D, modulates diverse biological processes including calcium homeostasis and immune function. Genetic variation involving VDR shows striking differences in allele frequency between populations and has been associated with disease susceptibility including tuberculosis and autoimmunity, although results have often been conflicting. We hypothesized that methylation of VDR may be population specific and that the combination of differential methylation and genetic variation may characterise TB predisposition. We use bisulphite conversion and/or pyrosequencing to analyse the methylation status of 17 CpGs of VDR and to genotype 7 SNPs in the 3′ CpG Island (CGI 1060), including the commonly studied SNPs ApaI (rs7975232) and TaqI (rs731236). We show that for lymphoblastoid cell lines from two ethnically diverse populations (Yoruba from HapMap, n=30 and Caucasians, n=30) together with TB cases (n=32) and controls (n=29) from the Venda population of South Africa there are methylation variable positions (MVPs) in the 3′ end that significantly distinguish ethnicity (9/17 CpGs) and TB status (3/17 CpGs). Moreover methylation status shows complex association with TaqI genotype highlighting the need to consider both genetic and epigenetic variants in genetic studies of VDR association with disease.

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Promoter methylation of E-cadherin gene in gastric mucosa associated with Helicobacter pylori infection and in gastric cancer

Cited by 270 publications

References 22 publications

Accumulation of DNA methylation is associated with tumor stage in gastric cancer

Accumulation of DNA methylation is associated with tumor stage in gastric cancer

DNA methylation profiles of gastric carcinoma characterized by quantitative DNA methylation analysis

Vitamin D receptor gene methylation is associated with ethnicity, tuberculosis, and TaqI polymorphism

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