Promoter of Vegetable Soybean GmTIP1;6 Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic Arabidopsis

Abstract

Tonoplast intrinsic proteins (TIPs), a sub-family of aquaporins (AQPs), are known to play important roles in plant abiotic stress responses. However, evidence for the promoters of TIPs involvement in abiotic stress processes remains scarce. In this study, the promoter of the vegetable soybean GmTIP1;6 gene, which had the highest similarity to TIP1-type AQPs from other plants, was cloned. Expression pattern analyses indicated that the GmTIP1;6 gene was dramatically induced by drought, salt, abscisic acid (ABA), and methyl jasmonate (MeJA) stimuli. Promoter analyses revealed that the GmTIP1;6 promoter contained drought, ABA, and MeJA cis-acting elements. Histochemical staining of the GmTIP1;6 promoter in transgenic Arabidopsis corroborated that it was strongly expressed in the vascular bundles of leaves, stems, and roots. Beta-glucuronidase (GUS) activity assays showed that the activities of the GmTIP1;6 promoter were enhanced by different concentrations of polyethylene glycol 6000 (PEG 6000), NaCl, ABA, and MEJA treatments. Integrating these results revealed that the GmTIP1;6 promoter could be applied for improving the tolerance to abiotic stresses of the transgenic plants by promoting the expression of vegetable soybean AQPs.

Cloning and functional analysis of the promoter of a UDP-glycosyltransferase gene from Panax quinquefolium L.

“…MeJA-responsive is common in the plant pathogen interaction genes promoters, has been demonstrated to improve plant resistance to environmental stresses by activate defense mechanisms(Zheng et al 2011;Su et al 2011;Li et al 2021). MeJA treatment produced a signi cant induction effect on the promoter of the vegetable soybean GmTIP1;6 gene and enhanced the activity of the GUS(Feng et al 2022), and the induction of MeJA also found in AtPROPEPs and AtPEPR promoter from Arabidopsis thaliana (Safaeizadeh M, Boller T 2019). Similar results were also found in this study, the GUS activity of Pq3-O-UGT2 promoter deletion fragments can be enhanced by 48 hours of MeJA treatment, and the signi cate change is P-1399::GUS with 2.12 times increased, much more than the other six 5′ terminal series deleted fragments.…”