Promoter polymorphism in FAE1.1 and FAE1.2 genes associated with erucic acid content in Brassica juncea

Saini, Neeru; Yashpal, .; Koramutla, Murali Krishna; Singh, Naveen; Singh, S. P.; Singh, Rajendra; Yadav, Sangita; Bhattacharya, Ramcharan; Vasudev, Sujata; Yadava, Devendra K.

doi:10.1007/s11032-019-0971-x

Cited by 13 publications

(13 citation statements)

References 26 publications

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“…CAPS markers for genes FAE1.1 and FAE1.2 were developed and successfully used in breeding programs in the improvement of B. juncea ( 104 ) and B. rapa ( 105 ), but when compared to simple PCR-based markers, CAPS markers are labor- and cost-intensive, which restrict their routine use by breeders. Recently, Saini et al ( 112 ) assessed polymorphism in the upstream region of FAE1.1 and FAE1.2 genes, across the low erucic acid and high erucic acid genotypes, and developed PCR-based markers (FAE1.1P and FAE1.2P) based on this variability. These markers can be effectively used in marker-assisted selection for the development of low-erucic-acid varieties in B. juncea.…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

et al. 2020

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Nutritional stress is making over two billion world population malnourished. Either our commercially cultivated varieties of cereals, pulses, and oilseed crops are deficient in essential nutrients or the soils in which these crops grow are becoming devoid of minerals. Unfortunately, our major food crops are poor sources of micronutrients required for normal human growth. To overcome the problem of nutritional deficiency, greater emphasis should be laid on the identification of genes/quantitative trait loci (QTLs) pertaining to essential nutrients and their successful deployment in elite breeding lines through marker-assisted breeding. The manuscript deals with information on identified QTLs for protein content, vitamins, macronutrients, micro-nutrients, minerals, oil content, and essential amino acids in major food crops. These QTLs can be utilized in the development of nutrient-rich crop varieties. Genome editing technologies that can rapidly modify genomes in a precise way and will directly enrich the nutritional status of elite varieties could hold a bright future to address the challenge of malnutrition.

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Section: Introductionmentioning

confidence: 99%

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

et al. 2020

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“…It has been proven that erucic acid content is governed by the fatty acid elongase 1 ( FAE1 ) gene that translates the enzyme β-ketoacyl-CoA synthase (KCS) in erucic acid biosynthesis alleyway and catalyzes the first four enzymatic reactions in the synthesis of very-long-chain monounsaturated fatty acids (VLCMFAs) [ 1 , 2 , 46 , 47 , 48 ]. The mutation in the FAE1 gene primes the forfeiture of occupation in enzymatic action and diminishes the gathering of VLCMFAs in seeds [ 1 , 2 , 49 , 50 ]. In the current research, plants with lower erucic acid have been obtained within R 0 and R 1 generations of putative somaclones raised from both the genotypes compared to mother plants, possibly due to mutations.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, applications of RAPD markers for the same purposes have been reported by other researchers [25,45] also. It has been proven that erucic acid content is governed by the fatty acid elongase 1 (FAE1) gene that translates the enzyme β-ketoacyl-CoA synthase (KCS) in erucic acid biosynthesis alleyway and catalyzes the first four enzymatic reactions in the synthesis of very-long-chain monounsaturated fatty acids (VLCMFAs) [1,2,[46][47][48]. The mutation in the FAE1 gene primes the forfeiture of occupation in enzymatic action and diminishes the gathering of VLCMFAs in seeds [1,2,49,50].…”

Section: Molecular Confirmation Of Putative Drought Tolerant Plant (S)mentioning

confidence: 99%

“…It is an oilseed in high demand proving an increasing trend by fulfilling demand and supply with desired qualitative traits. The occurrence of erucic acid (C22:1) in elevated concentration (35–50%) is a significant drawback to mustard oil [ 1 , 2 , 3 , 4 , 5 ]. This high concentration of erucic acid in edible mustard oil makes it nutritionally unfavorable for the human diet.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

Shyam¹,

Tripathi²,

Tiwari³

et al. 2021

Plants

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Brassica junceais a crucial cultivated mustard species and principal oilseed crop of India and Madhya Pradesh, grown for diverse vegetables, condiments, and oilseeds. Somaclonal variation was explored as a probable source of additional variability for the manipulation of fatty acids, especially low erucic acid contents that may be valuable for this commercially important plant species. The plantlets regenerated from tissue cultures (R0), their R1 generation and respective parental lines were compared for morpho-physiological traits and fatty acid profile for the probable existence of somaclonal variations. The first putative somaclone derived from genotype CS54 contained 5.48% and 5.52% erucic acid in R0 and R1 regenerants, respectively, compared to the mother plant (41.36%). In comparison, the second somaclone acquired from PM30 exhibited a complete absence of erucic acid corresponding to its mother plant (1.07%). These putative somaclones present a source of variation for exploitation in the development of future mustard crops with low erucic acid content.

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“…Cleaved amplified polymorphic sequences were also used to characterize FAE1.1 and FAE1.2 alleles in B. juncea ( Saini et al, 2016 ), but CAPS markers are dominant and these fail to identify recessive alleles in heterozygous state. Recently, Saini et al (2019) have reported PCR markers based on the sequence variation in the promoter regions of the FAE1 . These are gel-based markers.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Kompetitive Allele-Specific PCR Assays for Erucic Acid Content in Indian Mustard [Brassica juncea (L.) Czern and Coss.]

Gill

Kaur

et al. 2021

Front. Plant Sci.

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Brassica juncea L. is the most widely cultivated oilseed crop in Indian subcontinent. Its seeds contain oil with very high concentration of erucic acid (≈50%). Of late, there is increasing emphasis on the development of low erucic acid varieties because of reported association of the consumption of high erucic acid oil with cardiac lipidosis. Erucic acid is synthesized from oleic acid by an elongation process involving two cycles of four sequential steps. Of which, the first step is catalyzed by β-ketoacyl-CoA synthase (KCS) encoded by the fatty acid elongase 1 (FAE1) gene in Brassica. Mutations in the coding region of the FAE1 lead to the loss of KCS activity and consequently a drastic reduction of erucic acid in the seeds. Molecular markers have been developed on the basis of variation available in the coding or promoter region(s) of the FAE1. However, majority of these markers are not breeder friendly and are rarely used in the breeding programs. Present studies were planned to develop robust kompetitive allele-specific PCR (KASPar) assays with high throughput and economics of scale. We first cloned and sequenced FAE1.1 and FAE1.2 from high and low erucic acid (<2%) genotypes of B. juncea (AABB) and its progenitor species, B. rapa (AA) and B. nigra (BB). Sequence comparisons of FAE1.1 and FAE1.2 genes for low and high erucic acid genotypes revealed single nucleotide polymorphisms (SNPs) at 8 and 3 positions. Of these, three SNPs for FAE1.1 and one SNPs for FAE1.2 produced missense mutations, leading to amino acid modifications and inactivation of KCS enzyme. We used SNPs at positions 735 and 1,476 for genes FAE1.1 and FAE1.2, respectively, to develop KASPar assays. These markers were validated on a collection of diverse genotypes and a segregating backcross progeny. KASPar assays developed in this study will be useful for marker-assisted breeding, as these can track recessive alleles in their heterozygous state with high reproducibility.

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Promoter polymorphism in FAE1.1 and FAE1.2 genes associated with erucic acid content in Brassica juncea

Cited by 13 publications

References 26 publications

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

Development and Validation of Kompetitive Allele-Specific PCR Assays for Erucic Acid Content in Indian Mustard [Brassica juncea (L.) Czern and Coss.]

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