Promoter polymorphisms in the lncRNA-MIAT gene associated with acute myocardial infarction in Chinese Han population: a case–control study

Abstract

Background: Coronary atherosclerotic disease (CAD) is one of the greatest causes of death and disability around the world, and has emerged as a major public health problem. Acute myocardial infarction (AMI) is the most serious type of CAD. Myocardial infarction (MI) association transcript (MIAT) has demonstrated that it plays an important role in AMI. Purpose: To investigate the association between MIAT promoter polymorphisms and AMI in Chinese Han population. Methods: A total of 212 AMI patients and 218 healthy controls were recruited. The long non-coding RNA (lncRNA)-MIAT promoter polymorphisms (single nucleotide polymorphisms (SNPs)) were obtained using polymerase chain reaction (PCR) and sequencing techniques. Chi-square test was used to analyze the allele and genotype frequencies of each SNP in two groups. Logistic regression analysis was used to analyze the association of each SNP with AMI. Linkage disequilibrium (LD) and haplotype analysis were performed using SHEsis software. A JASPAR database search predicts transcription factors transition of linked polymorphism in MIAT promoter. Results: Ten SNPs were found, including rs56371714, rs55892869, rs151057042, rs2157598, rs150465374, rs5761664, rs8142890, rs5752375, rs9608515 and rs1055293700, whereas rs1055293700 was found only in the control group. Single and logistic regression analysis showed that there was a significant correlation between rs5752375 and rs9608515 polymorphisms and AMI, while other sites had no relationship with AMI. These MI association polymorphisms may change the binding sites with transcription factor. Conclusions: The polymorphisms of lncRNA-MIAT promoter rs5752375 and rs9608515 were significantly associated with AMI in Chinese Han population. This result would be of clinical importance for the early diagnosis of AMI.

Association of Angio-LncRNAs MIAT rs1061540/MALAT1 rs3200401 Molecular Variants with Gensini Score in Coronary Artery Disease Patients Undergoing Angiography

“…Recently, Ma et al investigated ten polymorphisms of MIAT promoters and found that two SNPs (rs5752375T/C and rs9608515T/C) were associated with MI, with the TT genotype being a risk factor compared with the CC genotype, potentiating the role of MIAT SNPs in CAD development [ 32 ].…”

“…It is worth noting that the functional role of MIAT polymorphisms might be related to gene expression alteration. A minor allele at exon 5 11,741 G>A SNP of MIAT increased its transcription compared with major allele G. Additionally, rs5752375 and rs9608515 polymorphisms of MIAT promotor might influence binding of the transcription factors, causing it to lose some and interact with others [ 31 , 32 ]. MIAT was highly expressed in the atheromatous plaques [ 33 ] and the peripheral blood of CAD patients.…”

The Long Non-coding Road to Atherosclerosis

“…LncRNA SMILR seems to play a role in plaque stability [58], and its plasma levels positively correlate with C-reactive protein [59]. SNPs in the lncRNA MIAT promoter correlated with acute myocardial infarction in a Chinese Han population [86], while a specific polymorphism in MALAT1 (rs619586AG/GG) might be CVD protective [87].…”

The Long Non-Coding RNA GOMAFU in Schizophrenia: Function, Disease Risk, and Beyond

“…While the previous study focused on the SNPs within the GOMAFU transcript, another study analyzed whether SNPs near the GOMAFU transcript were associated with acute myocardial infarction (AMI) [ 137 ]. This study examined the proximal promoter region of GOMAFU through PCR amplification using genomic DNA isolated from the peripheral leukocytes of controls and AMI patients in a Chinese Han population.…”

“…This study examined the proximal promoter region of GOMAFU through PCR amplification using genomic DNA isolated from the peripheral leukocytes of controls and AMI patients in a Chinese Han population. Comparing the SNPs identified from the control and patient group, two SNPs were significantly associated with AMI, rs5752375, and rs9608515 ( Table 1 and Figure 2 ) [ 137 ]. These two SNPs are located 938 and 864 bp upstream of the GOMAFU transcription start site, respectively, likely within the GOMAFU promoter.…”