Promoters ofAaGL2andAaMIXTA-Like1genes ofArtemisia annuadirect reporter gene expression in glandular and non-glandular trichomes

Jindal, Sunita; Longchar, Bendangchuchang; Singh, Alka; Gupta, Vikrant

doi:10.1080/15592324.2015.1087629

Cited by 12 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even in Artemisia annua promoters of AaGL2 and AaMIXTA LIKE drive the expression of reporter gene in non-glandular trichomes of Arabidopsis, as well as in both glandular and T shaped trichomes of Artemisia annua (Sunita Jindal et al 2015). Since the expression of ATGL2 is controlled by WRKY transcription factor TTG2 by binding to W box (TGACT) found in the promoter of ATGL2 (Ishida et al 2007), and the presence of W box has been reported in the promoter of AaGL2) Jindal et al 2015). In addition to this overexpression of WD 40 repeat from Artemisia annua rescued the trichomeless phenotype of ttg1 mutant in Arabidopsis (Wang et al 2016).…”

Section: Conservation Among Regulators Of Trichome Development In Plantsmentioning

confidence: 86%

Transcriptional regulation of trichome development in plants: an overview

Khan

Mohammad

Hurrah

et al. 2021

Proc.Indian Natl. Sci. Acad.

View full text Add to dashboard Cite

Trichomes are specialized structures derived from epidermal cells. Apart from providing protection to plants, trichomes are source of medicinally important compounds. Although genes involved in initiation and patterning of trichomes have been characterized in some plants like Arabidopsis thaliana, but our knowledge about the development of these trichomes is still limited. Recent studies confirm the role of different transcription factors in trichome development of plants. In model plant Arabidopsis different transcription factor families have been studied in detail. It has been found that transcription factors like bHLH, R2R3 MYB form an activation complex with TTG1 which initiate trichome formation while as R3 MYB type transcription factors inhibit the formation of activation complex. The activation complex acts as a major trigger in the regulation of trichome development. It would be interesting to know whether such activation complex occurs in other plants like Nicotiana tobaccum, Lycopersicum esculentum, Artemisia annua etc. In this review, we focus on recent updates on transcriptional regulation of trichome development and conservation of these regulators in various species of pharmacologically and economically important plants. We have briefly discussed the cross talk of transcription factors with different hormones during trichome development. Further we have elaborated fundamental research ideologies on trichome development and translation of this research from model plant to plants of industrial interest.

show abstract

Section: Conservation Among Regulators Of Trichome Development In Plantsmentioning

confidence: 86%

Transcriptional regulation of trichome development in plants: an overview

Khan

Mohammad

Hurrah

et al. 2021

Proc.Indian Natl. Sci. Acad.

View full text Add to dashboard Cite

show abstract

“…One way involves the genetic ablation of GSTs in peripheral organs. Several promoters upstream of trichome genes in tobacco, tomato, cotton, and Artemisia annua have been shown to induce reporter gene expression in GSTs 38 – 42 ; thus, these promoters could be used for the genetic ablation of GSTs.…”

Section: Discussionmentioning

confidence: 99%

Reduction in organ–organ friction is critical for corolla elongation in morning glory

et al. 2021

View full text Add to dashboard Cite

In complex structures such as flowers, organ–organ interactions are critical for morphogenesis. The corolla plays a central role in attracting pollinators: thus, its proper development is important in nature, agriculture, and horticulture. Although the intraorgan mechanism of corolla development has been studied, the importance of organ–organ interactions during development remains unknown. Here, using corolla mutants of morning glory described approximately 200 years ago, we show that glandular secretory trichomes (GSTs) regulate floral organ interactions needed for corolla morphogenesis. Defects in GST development in perianth organs result in folding of the corolla tube, and release of mechanical stress by sepal removal restores corolla elongation. Computational modeling shows that the folding occurs because of buckling caused by mechanical stress from friction at the distal side of the corolla. Our results suggest a novel function of GSTs in regulating the physical interaction of floral organs for macroscopic morphogenesis of the corolla.

show abstract

“…Interestingly, Jindal et al . () cloned a MIXTA homolog, AaMIXTA‐like1, from A. annua and used a transient assay to show that its promoter was able to direct expression of a reporter in both GST and TSTs, but no functional analysis of the gene was undertaken.…”

Section: Discussionmentioning

confidence: 99%

“…These differences suggest that the molecular mechanism for formation of multicellular trichomes in A. annua may be very distinct from the one involved in formation of unicellular trichomes in Arabidopsis. Interestingly, Jindal et al (2015) cloned a MIXTA homolog, AaMIXTA-like1, from A. annua and used a transient assay to show that its promoter was able to direct expression of a reporter in both GST and TSTs, but no functional analysis of the gene was undertaken.…”

Section: Researchmentioning

confidence: 99%

The roles of AaMIXTA1 in regulating the initiation of glandular trichomes and cuticle biosynthesis in Artemisia annua

et al. 2017

View full text Add to dashboard Cite

SummaryThe glandular secretory trichomes (GSTs) on Artemisia annua leaves have the capacity to secrete and store artemisinin, a compound which is the most effective treatment for uncomplicated malaria. An effective strategy to improve artemisinin content is therefore to increase the density of GSTs in A. annua. However, the formation mechanism of GSTs remains poorly understood.To explore the mechanisms of GST initiation in A. annua, we screened myeloblastosis (MYB) transcription factor genes from a GST transcriptome database and identified a MIXTA transcription factor, AaMIXTA1, which is expressed predominantly in the basal cells of GST in A. annua. Overexpression and repression of AaMIXTA1 resulted in an increase and decrease, respectively, in the number of GSTs as well as the artemisinin content in transgenic plants.Transcriptome analysis and cuticular lipid profiling showed that AaMIXTA1 is likely to be responsible for activating cuticle biosynthesis. In addition, dual-luciferase reporter assays further demonstrated that AaMIXTA1 could directly activate the expression of genes related to cuticle biosynthesis.Taken together, AaMIXTA1 regulated cuticle biosynthesis and prompted GST initiation without any abnormal impact on the morphological structure of the GSTs and so provides a new way to improve artemisinin content in this important medicinal plant.

show abstract

Promoters ofAaGL2andAaMIXTA-Like1genes ofArtemisia annuadirect reporter gene expression in glandular and non-glandular trichomes

Cited by 12 publications

References 41 publications

Transcriptional regulation of trichome development in plants: an overview

Transcriptional regulation of trichome development in plants: an overview

Reduction in organ–organ friction is critical for corolla elongation in morning glory

The roles of AaMIXTA1 in regulating the initiation of glandular trichomes and cuticle biosynthesis in Artemisia annua

Contact Info

Product

Resources

About