2003
DOI: 10.1073/pnas.0337404100
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Promoters of the murine embryonic β-like globin genes Ey and β h1 do not compete for interaction with the β-globin locus control region

Abstract: Mammalian ␤-globin loci contain multiple ␤-like genes that are expressed at different times during development. The murine ␤-globin locus contains two genes expressed during the embryo stage, Ey and ␤h1, and two genes expressed at both the fetal and postnatal stages, ␤-major and ␤-minor. Studies of transgenic human ␤-like globin loci in mice have suggested that expression of one gene at the locus will suppress expression of other genes at the locus. To test this hypothesis we produced mouse lines with deletion… Show more

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Cited by 11 publications
(14 citation statements)
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“…As reported previously, 15 the transcription of ␤h1 was not changed by the deletion of the Ey promoter. Hence, the transcription level of ␤h0 could be compared with that of ␤h1 directly, even on the ⌬Ey allele.…”
Section: Transcription Of Other ␤-Type Globin Genes In the Ey And ␤H1supporting
confidence: 56%
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“…As reported previously, 15 the transcription of ␤h1 was not changed by the deletion of the Ey promoter. Hence, the transcription level of ␤h0 could be compared with that of ␤h1 directly, even on the ⌬Ey allele.…”
Section: Transcription Of Other ␤-Type Globin Genes In the Ey And ␤H1supporting
confidence: 56%
“…Each deleted region roughly measured 1.1 kbp, spanning from 700 bp 5Ј to the transcription start site to near the 3Ј end of exon 2 of each gene. 15 One of the ES clones previously targeted to replace the ␤h1 promoter with neo (⌬␤h1neo) was retargeted with the construct that replaced the Ey promoter with hygro (⌬Eyhy-gro). ES clones were screened for targeting of Ey, and Southern blots were used to identify clones that were targeted on both alleles in cis rather than in trans ( Figure 1B).…”
Section: Production Of the Double Embryonic Gene Promoter Deletion Micementioning
confidence: 99%
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“…The ␤-globin gene promoters are the most obvious candidate sequences. As such, we examined histone modifications across ␤-globin loci harboring deletions of the embryonic ␤-globin gene promoters.Targeted deletion of the ⑀y-and ␤h1-globin gene promoters, alone and in combination, has been performed previously 28,29 ( Figure 4A). In agreement with these studies, we find that each promoter deletion eliminates expression of the corresponding ␤-globin gene, but expression of the other globin genes is either not affected (⑀y in the ␤h1 promoter deletion, ␤h1 in the ⑀y promoter deletion), or slightly affected (␤1/␤2 expression in the ⑀y/␤h1 double promoter deletion) ( Figure 4B).…”
mentioning
confidence: 99%