Promoting effects of organic medium supplements on the micropropagation of promising ornamental Daphne species (Thymelaeaceae)

Wiszniewska, Alina; Hanus-Fajerska, Ewa; Grabski, Krzysztof; Tukaj, Zbigniew

doi:10.1007/s11627-012-9480-x

Cited by 20 publications

(18 citation statements)

References 34 publications

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“…Such mechanism may explain rhizogenic ability in WD shoots induced on auxin-free media. Considering the root-promoting character of supplements tested, our previous studies revealed that natural auxins are not likely to be present in dialyzed pineapple (Wiszniewska et al 2013). Their presence in the conditioned medium obtained from green alga Desmodesmus subspicatus has not been confirmed either.…”

Section: Discussionmentioning

confidence: 91%

“…Pineapple pulp was prepared according to Kitsaki et al (2004). The dialyzate of pineapple pulp and conditioned medium after the green alga Desmodesmus subspicatus culture (CM) were produced as described earlier (Grabski and Tukaj 2008;Wiszniewska et al 2013). After 7 days the shoots were transferred to a hormone-free MS medium for root elongation.…”

Section: Rooting Protocolmentioning

confidence: 99%

“…Therefore, such products provide an alternative to synthetic agrochemicals used in crop production (Saxena and Pandey 2001). Recently, the biological activity of natural additives was evaluated in tissue cultures of numerous plant species, including agricultural crops such as pea (Molnar and Ö rdög 2005) and date palm (Al-Khayri 2011), medicinal plants (Amin et al 2009) and ornamentals (Kaur and Bhutani 2012;Wiszniewska et al 2013). It has been proved that the use of phytoactive medium supplements promotes organogenesis, differentiation of cultured tissues, as well as the ability of the obtained microplantlets to acclimatize.…”

Section: Introductionmentioning

confidence: 99%

“…pineapple pulp dialyzate and conditioned medium containing green algae exudates, during in vitro rooting of the common plum (Prunus domestica L.). Both additives examined were recently found to promote rooting in woody Daphne species (Wiszniewska et al 2013). Therefore, the current study was designed to assess their suitability as potential rooting stimulators in woody plants.…”

Section: Introductionmentioning

confidence: 99%

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Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Wiszniewska

Nowak

Kołton

et al. 2016

Plant Cell Tiss Organ Cult

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The study aimed at evaluating the response of common plum (Prunus domestica L.) microshoots during in vitro rooting in the presence of two phytoactive medium supplements, i.e. a dialyzate of pineapple pulp and a conditioned medium containing green algae Desmodesmus subspicatus exudates. Rooting efficiency was evaluated after 4 weeks of culture. During the root induction phase the content of phenolic compounds in shoot bases was determined and anatomical studies were conducted. Medium supplements were analyzed for the content of carbohydrates and phenolic acids. Both supplements were efficient in rooting induction of shoots of a difficult-to-root cultivar 'Węgierka Zwykła'. Medium supplementation allowed a significant reduction in the exogenous auxin content required for rooting. The highest rooting efficiencies on supplemented media were 28.9 and 27.8 %, in comparison with 33.3 % obtained in the control medium with doubled concentration of exogenous auxins. In the easy-to-root plum cultivar 'Węgierka Dąbrowicka' the rooting rate was slightly reduced in the presence of pineapple dialyzate, while in the presence of algal conditioned medium the rooting rate decreased substantially compared with the nonsupplemented medium. Approximately 30 % of 'Węgierka Dąbrowicka' shoots rooted on supplemented auxin-free media. The content of phenolic compounds accumulated in shoot bases during the root induction stage reflected the differences in rooting ability between both plum cultivars, indicating potential stressful conditions of the culture generated by the presence of phytoactive natural supplements. Anatomical study allowed to recognize the mode of dedifferentiation leading to adventitious rhizogenesis in the common plum. The results are discussed in relation to the composition of medium supplements and their potential root-promoting activity.

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Section: Discussionmentioning

confidence: 91%

Section: Rooting Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Wiszniewska

Nowak

Kołton

et al. 2016

Plant Cell Tiss Organ Cult

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“…Despite the potential usefulness of such media in biotechnology, there are few papers on the interaction of algal extracellular matter with terrestrial plant growth. Preliminary studies indicate that algal extracellular exudates are indeed able to stimulate the growth of plant cells, even in a phylogenetically distinct higher plant such as Silene vulgaris (Caryophyllaceae) (Hanus-Fajerska et al, 2009) or in Daphne species (Wiszniewska et al, 2013). Previous studies have examined the effects on higher plants of compound(s) released into medium by ubiquitously occurring phytoplankton in suspension cell cultures and organ or embryo cultures (Hanus-Fajerska et al, 2009;Shrivastava and Banerjee, 2009;Gurusaravanan et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

Zielińska¹,

Matusiak-Mikulin²,

Grabski³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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Conditioned medium (CM) is a general term describing media in which cells have already been cultivated for some time. Such media, usually clarified by filtration, have been used by plant biotechnologists as additives supporting the growth of cell suspensions, organs and whole plants. This study examined the effect of CM obtained from green alga Desmodesmus subspicatus on the growth and functioning of the photosynthetic apparatus of Nicotiana tabacum and Arabidopsis thaliana in culture in vitro. Plants where cultured on CM diluted 1.25-, 2-and 5-fold with MS medium. The increase in fresh and dry weight was highest in tobacco and Arabidopsis cultured on CM/2 and CM/1.25 media. Those two concentrations also increased the amount of chlorophylls in both plants tested. CM improved parameter PI (reflecting the photosynthetic "vitality" of the organism) and electron transport efficiency, and increased the fraction of active reaction centers. Analysis of chlorophyll fluorescence in vivo suggests that the improvement of these plants grown in the presence of algal CM may result from stimulation of photosynthesis. Algal CM offers a convenient, cheap, universal supplement for stimulating the growth of higher plants in vitro.K Ke ey y w wo or rd ds s: : Algal exudates, in vitro culture, growth improvement, photosynthesis.

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Rhizosphere Engineering and Agricultural Productivity

Parray¹,

Mir

Shameem

2019

Sustainable Agriculture: Biotechniques in Plant Biology

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Promoting effects of organic medium supplements on the micropropagation of promising ornamental Daphne species (Thymelaeaceae)

Cited by 20 publications

References 34 publications

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

Rhizosphere Engineering and Agricultural Productivity

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