Promotion effect of sulfite on deoxyosones and 4-methylimidazole in caramel model system

Abstract

In this study, hydrogen-deuterium (H/D) exchange experiment was carried out to reveal the promotion effect of sulfite on the formation of deoxyosones and 4-methylimidazole (4-MeI) in the Maillard reaction. Glucose-ammonium (40mmol/L, pH 7.4 in PBS) model systems with different levels of sulfite were incubated at 110°C for 2h. Alpha-dicarbonyls were detected after derivatization by a high-performance liquid chromatography with a diode array detector (HPLC-DAD). 4-MeI in the Maillard reaction was tested using a high-performance anion exchange chromatography with an electrochemical detector (HPAEC-ED). The H/D exchange ratios of hexose (fructose, glucose and mannose) were tested by HPAEC-MS. Results showed that the aldo-enol transition of enediol was promoted by sulfite, which promoted the formation of deoxyosones and 4-MeI. In addition, the oxidation reaction of enediols was inhibited by the antioxidant sulfite, which caused the inhibition of osones formation in the Maillard reaction.

Mechanism of Carbon Skeleton Formation of 2,3,5-Trimethylpyrazine via a Conversion Reaction between Methylglyoxal and Glyoxal

“…A total of 8 μL of internal standard (3,4-hexanedione, 25 g/L) and 1 mL of derivatization reagent obtained from o -phenylenediamine (2%) and solubilized in HEPES (1 mol/L, pH 7.0) at 11 mmol/L DTPA were added to the reaction solutions of methylglyoxal/ammonium sulfide (1 mL). The reaction mixtures were incubated in the dark for 2 h at room temperature (about 15 °C) and subsequently cleaned by C18-SPE (Agela Technologies, Tianjin, China). , The reaction solutions of glyoxal/ammonium sulfide and formaldehyde/ammonium sulfide were similarly treated.…”

“…The reaction mixtures were incubated in the dark for 2 h at room temperature (about 15 °C) and subsequently cleaned by C18-SPE (Agela Technologies, Tianjin, China). 23,24 The reaction solutions of glyoxal/ammonium sulfide and formaldehyde/ammonium sulfide were similarly treated. The cleaned mixtures were analyzed using HPLC (Agilent Technologies, Inc.) with a SPD-M20A DAD (Agilent Technologies, Inc.) system.…”