Promotion of follicular antrum formation by pig oocytes <i>in vitro</i>

Shen, Xiangju; Miyano, Takashi; Kato, Seishiro

doi:10.1017/s0967199400005074

Cited by 20 publications

(22 citation statements)

References 21 publications

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“…Conversely, the oocyte also affects the development and function of granulosa cells. The oocyte has been shown to stimulate granulosa cell proliferation and to promote the differentiation of the cumulus cell phenotype in antral follicles [42]. Therefore, the gap junction channels form a metabolic syncytium within the follicle, whereby the granulosa and cumulus cells provide essential metabolites to the oocyte [14] and presumably vise versa, as well.…”

Section: Discussionmentioning

confidence: 99%

Expression of Gap Junction Protein Connexin 43 during Follicular Atresia in the Ovary of Swamp Buffaloes

Feranil

Isobe

Nakao

2005

Journal of Reproduction and Development

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Abstract. The present study was performed to detect the presence of gap junction protein connexin 43 (Cx43) and describe the changes in its expression during ovarian follicular atresia in the swamp buffalo in comparison with cattle. Ovaries of Philippine swamp buffaloes (Bubalus bubalis; SB) and Holstein-Friesian cows (Bos taurus; HF) were collected from slaughterhouses, fixed in 10% formalin in PBS and embedded in paraffin. Sections of healthy follicles and at various follicular stages of atresia were immunostained with anti-Cx43 antibody. Cx43 appeared as punctate staining between granulosa cells (healthy to advanced atretic follicles), indicating assembled gap junctions, but was absent in the theca interna. In SB as well as in HF, granulosa cells showed a dense, moderate, and sparse immunoreactivity to Cx43 in healthy, early atretic, and advanced atretic follicles, respectively. Cumulus cells (in the advanced atretic follicle) surrounding oocytes and adjacent granulosa layers retain the Cx43 protein, although there was only a sparse expression of Cx43 observed in the granulosa layers distant from oocytes in the same follicles. The results indicate that gap junction protein Cx43 decreases in association with atresia and supports the concept that a loss of gap junctional communication plays a coordinating role in the process of atresia. Furthermore, the schema of Cx43 immunoreactivity in SB granulosa cells is similar to that of HF.

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Section: Discussionmentioning

confidence: 99%

Expression of Gap Junction Protein Connexin 43 during Follicular Atresia in the Ovary of Swamp Buffaloes

Feranil

Isobe

Nakao

2005

Journal of Reproduction and Development

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“…Similarly, a high concentration of [91,92]. Moreover, the presence of an oocyte is essential for development of an antral cavity [93].…”

Section: Oocytesmentioning

confidence: 99%

“…In addition, oocyte-derived factors alter cumulus cell activities such as specific amino acid transporter expression [89], cholesterol biosynthesis [90], and glycolysis [91,92]. Moreover, the presence of an oocyte is essential for development of an antral cavity [93].…”

Section: Oocyte-granulosa Cell Interactionsmentioning

confidence: 99%

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Isolation of Ovarian Components Essential for Growth and Development of Mammalian Oocytes <i>In Vitro</i>

Hirao

2012

Journal of Reproduction and Development

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Abstract. Mammalian ovaries contain a large number of oocytes, most of which degenerate either before or at various stages of growth. Dynamic and precise regulation in the ovary involves many factors, each with a unique role. Identifying the single most important factor is impossible; however, it may be possible to identify factors essential for oocyte growth. It is evident that oocytes can grow into competent ova in vitro; however, how faithfully the follicle should mimic the in vivo conditions remains unclear. In the culture system discussed in this review, bovine and mouse oocyte-granulosa cell complexes, at approximately the late mid-growth stage, spread on a substratum without the involvement of theca cells. The structural simplicity of this system is advantageous because it reduces the basic conditions essential for regulation of oocyte growth. Apart from biological factors, high concentrations of polyvinylpyrrolidone (molecular weight: 360000) improved oocyte growth. Among ovarian factors, androstenedione was used to compensate for the absence of theca cells, and it promoted both follicular growth and acquisition of oocyte meiotic competence. Most oocytes cultured in a group were viable after long-term culture, suggesting that unlike ovarian events, there was no exhaustive follicle selection. Collectively, oocytes and their associated granulosa cells can establish independent units capable of supporting oocyte growth in appropriately modified culture media. Key words: In vitro, Oocyte-granulosa cell complexes, Oocyte growth, Polyvinylpyrrolidone (J. Reprod. Dev. 58: [167][168][169][170][171][172][173][174] 2012) I n the mammalian ovary, a small proportion of primordial oocytes periodically enter the growth phase and even fewer grow, mature, and ovulate [1]. Various factors, including gonadotrophins, growth factors, and steroid hormones, underlie the dynamic regulation of oocyte and follicular growth and their selection. The regulation is so dynamic and precise that examination of these events in situ is extremely difficult.One solution to this problem is to isolate oocytes and their associated ovarian cells and culture them in vitro. It is well established that oocytes can grow in vitro because "normal" ova have been produced in vitro in mice [2][3][4][5][6] and cattle [7,8] (oocytes were cultured for the latter half of their growth period). In mice, offspring have even been derived from oocytes cultured from primordial follicles [9,10]. It is thus clear that requirements for oocyte growth can be studied in vitro. In addition, it is possible to isolate ovarian components essential for oocyte growth and acquisition of developmental competence in vitro. However, how faithfully the follicle should mimic the in vivo conditions remains unclear. This review focuses on culture systems with the smallest possible functional structure effective to support oocyte growth. Two Major Types of Culture SystemsSeveral culture systems have been developed for in vitro oocyte growth [11]. They can be broadly divided into ...

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“…FSH is thought to affect granulosa cells by activating adenylyl cyclase activity and promoting cAMP accumulation (Kolena and Channing, 1972). Shen et al (1998) reported that the dbcAMP derivative of cAMP, rather than FSH, induces antrum formation in porcine COCs. However, little information concerning the effects of hormone supplementation and meiotic arresting substances on antrum formation and oocyte development is currently available in the bovine species because the studies of follicle and oocyte development in vitro have been conducted mainly in the mouse.…”

mentioning

confidence: 99%

Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

Ono

Takaoka

Morita

et al. 2015

Acta Veterinaria Hungarica

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This study evaluated the effect of dibutyryl cyclic adenosine monophosphate (dbcAMP) and human chorionic gonadotropin (hCG) on the formation of antral follicle-like structures (AFLSs) and on the meiotic status of bovine cumulus-oocyte complexes (COCs) embedded in collagen gel. Supplementation with dbcAMP increased the mean diameter of AFLSs during days 4-8 of culture compared with that of control COCs, irrespective of the concentration of dbcAMP used (0.5-2.0 mM). When the embedded COCs were cultured for 8 days with hCG, the diameters of AFLSs after 4 days of culture tended to be lower in the supplemented COCs than in the control COCs without hCG, irrespective of the concentration used (1-100 IU/mL). Supplementation with 10 IU/mL hCG increased the concentrations of anti-Müllerian hormone but not progesterone and oestradiol in the culture medium after 4 days of culture. Almost all oocytes collected from AFLSs had resumed meiosis by the end of culture, irrespective of supplementation of dbcAMP and hCG. These results indicate that although dbcAMP had a positive effect on AFLS formation and development, supplementation with hCG was detrimental. Moreover, hCG supplementation did not influence the luteinisation of granulosa cells in the AFLS for 4 days after the start of culture.Key words: Antrum-like structures, cAMP, cumulus cells, gap junction, follicular formation Three-dimensional (3-D) culture of cumulus-oocyte complexes (COCs) may provide a valuable approach for studying the regulation of follicular development. When the 3-D organisation of cumulus granulosa cells around the oocyte was maintained during oocyte culture, COCs formed an antral follicle-like structure (AFLS) and grew in vitro (Harada et al., 1997). The formation of an AFLS is dependent on the proliferation and differentiation of cumulus granulosa

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Promotion of follicular antrum formation by pig oocytes in vitro

Cited by 20 publications

References 21 publications

Expression of Gap Junction Protein Connexin 43 during Follicular Atresia in the Ovary of Swamp Buffaloes

Expression of Gap Junction Protein Connexin 43 during Follicular Atresia in the Ovary of Swamp Buffaloes

Isolation of Ovarian Components Essential for Growth and Development of Mammalian Oocytes <i>In Vitro</i>

Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

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