Promotion of growth and biocontrol of brown patch disease by inoculation of Paenibacillus ehimensis KWN38 in bentgrass

Jeon, Seung Woo; Naing, Kyaw Wai; Lee, Yong Seong; Nguyen, Xuan Hoa; Kim, Kil Yong

doi:10.1007/s13580-015-0109-y

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…This approach uses microorganism(s) to manage plant disease by reducing pathogen activities and inducing plant defense mechanisms ( Nelson et al., 1994 ). Various microorganisms such as Paenibacillus ehimensis , Stenotrophomonas maltophilia , and Trichoderma harzianum have been characterized for controlling brown patch ( Yuen and Zhang, 2001 ; Guerrero et al., 2008 ; Jeon et al., 2015 ), but most commercially available biological products (active ingredients) for brown patch management are Bacillus subtilis (e.g. strain QST713, Rhapsody from Bayer; strain GB03, Companion from Growth Products Ltd).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method

Lee

Choi²,

Liu³

et al. 2023

Front. Plant Sci.

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Brown patch caused by the basidiomycete fungus Rhizoctonia solani is an economically important disease of cool-season turfgrasses. In order to manage the disease, different types of fungicides have been applied, but the negative impact of fungicides on the environment continues to rise. In this study, the beneficial bacteria Bacillus velezensis GH1-13 was characterized as a potential biocontrol agent to manage brown patch disease. The strain GH1-13 strongly inhibited the mycelial growth of turf pathogens including different anastomosis groups of R. solani causing brown patch and large patch. R. solani AG2-2(IIIB) hyphae were morphologically changed, and fungal cell death resulted from exposure to the strain GH1-13. In addition, the compatibility of fungicides with the bacterial strain, and the combined application of fungicide azoxystrobin and the strain in brown patch control on creeping bentgrass indicated that the strain could serve as a biocontrol agent. To develop strain-specific detection method, two unique genes from chromosome and plasmid of GH1-13 were found using pan-genome analysis of 364 Bacillus strains. The unique gene from chromosome was successfully detected using both SYBR Green and TaqMan qPCR methods in bacterial DNA or soil DNA samples. This study suggests that application of GH1-13 offers an environmentally friendly approach via reducing fungicide application rates. Furthermore, the developed pipeline of strain-specific detection method could be a useful tool for detecting and studying the dynamics of specific biocontrol agents.

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Section: Introductionmentioning

confidence: 99%

Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method

Lee

Choi²,

Liu³

et al. 2023

Front. Plant Sci.

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“…Rhizoctonia solani is a widespread soil‐borne fungal species affecting many crops, has a wide host range and causes various plant diseases such as brown patch in turfgrass , damping off , as well as black scurf in potatoes , bare patch in cereals , root rot in sugar beet , belly rot in cucumber , and sheath blight in rice . In recent years, although Rhizoctonia disease is managed by cultural practices , chemical synthetic fungicides are frequently used to control this disease .…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of N‐butyl‐tetrahydro‐5‐oxofuran‐2‐carboxamide produced by Bacillus sp. L60 and its antifungal activity

et al. 2017

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Rhizoctonia solani is the cause of substantial economic loss in many crops. The aim of this study is to investigate biocontrol potential of Bacillus sp. L60 against R. solani and to purify an antifungal compound. In this study, Bacillus sp. L60 demonstrated significant antagonism toward R. solani with the dual culture assay. The antifungal compound was extracted from Bacillus sp. L60 culture supernatant with n-butanol, and identified as N-butyl-tetrahydro-5-oxofuran-2-carboxamide (BT-5O-2C) having molecular weights of 185.1052 Da with the formula C H NO using NMR and HR-ESI-MS analysis. The minimum inhibitory concentration (MIC) value of the antifungal compound was 256 µg ml against R. solani. Therefore, our results clearly demonstrated BT-5O-2C as well as Bacillus sp. L60 as potential biological control agents for the management of R. solani.

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“…Sclerotinia homoeocarpa is the most economically important disease that affects creeping bentgrass [ 13 , 15 ] and is also widespread within the grass family [ 16 ]. Rhizoctonia solani is another major fungal pathogen that affects turfgrass species including creeping bentgrass [ 17 ]. R. solani affects diverse crops including potato, tomato, cucumber, green pea and rice [ 18 – 23 ].…”

Section: Introductionmentioning

confidence: 99%

Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities

et al. 2016

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BackgroundEndophytes are microbes that inhabit internal plant tissues without causing disease. Plant microbial communities consist of large numbers of endophyte species. Understanding the functions of these endophytes is a major challenge. An important function of some endophytes is to suppress fungal pathogens. Typically, plant associated microbes are screened for anti-fungal activities in vitro using the high-throughput dual culture screen, but it is not clear whether this method correlates with the activities of these microbes in planta. Furthermore, it is not clear whether in vitro screening captures all of the microbes that show this activity inside plants. The objective of this study was to evaluate the relevance of the in vitro dual culture method for screening endophytes with anti-fungal activity.ResultsIn parallel, 190 bacterial endophytes from the corn grass family (Zea) were screened for suppression of two fungal pathogens (Sclerotinia homoeocarpa and Rhizoctonia solani) using the in vitro dual culture method, and in planta using the model plant, creeping bentgrass. All endophytes that showed anti-fungal activity in planta against Sclerotinia homoeocarpa and Rhizoctonia solani (3 or 4 strains, respectively, out of 190), were captured in vitro. The in vitro and in planta screening results strongly correlated (r = 0.81 and r = 0.94 for the two pathogens, respectively).ConclusionsEvidence was gained here that the in vitro dual culture method is a relevant method for high throughput screening of plant endophyte communities for anti-fungal activity. In our study, the method captured all of the microbes that suppressed the corresponding pathogens in planta.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0623-9) contains supplementary material, which is available to authorized users.

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Promotion of growth and biocontrol of brown patch disease by inoculation of Paenibacillus ehimensis KWN38 in bentgrass

Cited by 7 publications

References 30 publications

Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method

Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method

Isolation and identification of N‐butyl‐tetrahydro‐5‐oxofuran‐2‐carboxamide produced by Bacillus sp. L60 and its antifungal activity

Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities

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