2003
DOI: 10.1002/ijc.11270
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Promotion of intestinal tumor formation by inulin is associated with an accumulation of cytosolic β‐catenin in Min mice

Abstract: Inulin, polydisperse ␤ (2-1) fructan, has been suggested to protect against colon carcinogenesis and is currently used in a number of food applications. However, the data regarding the role of inulin in intestinal carcinogenesis remains controversial since the results of our previous study suggested that inulin promotes intestinal tumor formation in Min mice, an animal model for intestinal cancer with a mutation in the Apc tumor suppressor gene (Carcinogenesis 2000;21:1167-73). In our present study, we further… Show more

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Cited by 50 publications
(47 citation statements)
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“…These data not only correlated with clinical signs of diarrhea but also help explain the initial fluid accumulation observed in previous studies. 14,39 Thus, taken together with prior data, the findings presented here suggest that NF-B activation opens paracellular spaces and promotes movement of fluid into bowel lumen.…”
Section: Discussionsupporting
confidence: 76%
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“…These data not only correlated with clinical signs of diarrhea but also help explain the initial fluid accumulation observed in previous studies. 14,39 Thus, taken together with prior data, the findings presented here suggest that NF-B activation opens paracellular spaces and promotes movement of fluid into bowel lumen.…”
Section: Discussionsupporting
confidence: 76%
“…39 Tissue samples were homogenized in 5 ml of 20 mmol/L Tris-HCl, ph7.4, 2 mmol/L EDTA, 10 mmol/L EGTA, 0.25 mol/L sucrose, and protease inhibitor cocktail (Sigma), and centrifuged at 200 ϫ g at 4°C for 10 minutes. The supernatant was centrifuged at 10 3 ϫ g at 4°C for 10 minutes followed by ultracentrifugation at 10 5 ϫ g at 4°C for 1 hour.…”
Section: Subcellular Fractionationmentioning
confidence: 99%
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“…The effects of coffee on b-catenin and cyclin D1 in tumours were therefore analysed by a semi-quantitative Western blot method. As observed in a previous study (24), immunoblots of b-catenin gave one band at 98 kDa in rat brain homogenate (control), and one band (or two bands) at 92 kDa (full-length b-catenin) in the adenoma tissue of Apc Min/' mice ( Fig. 2A).…”
Section: B-catenin and Cyclin D1 Analysissupporting
confidence: 84%
“…Specificities of signals were verified either with incubations without appropriate primary antibody or by using a blocking peptide provided by a supplier (Lab Vision, Fremont, CA, USA). The specificity of the b-catenin bands was ensured earlier by using two other commercially available antibodies (24). Blots were scanned and analysed using a GS-800 Calibrating …”
Section: Western Blot Analysismentioning
confidence: 99%