Promotion of Momordica Charantia polysaccharides on neural stem cell proliferation by increasing SIRT1 activity after cerebral ischemia/reperfusion in rats

Ma, Juyun; Fan, Haidi; Cai, Heng; Hu, Zhaoli; Zhou, Xiaoling; Li, Fengying; Chen, Hansen; Shen, Jiangang; Qi, Suhua

doi:10.1016/j.brainresbull.2021.02.016

Cited by 27 publications

(17 citation statements)

References 61 publications

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“…the proliferation of endogenous NSCs after cerebral I/R. Our previous research found that Momordica charantia polysaccharides promote endogenous NSC proliferation through the SIRT1/β-catenin pathway after cerebral I/R injury [44]. Theaflavin enhanced NSC proliferation by targeting miRNA-128-3p and further activating the Nrf2 pathway to reduce oxidative stress [45].…”

Section: Discussionmentioning

confidence: 99%

KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA

et al. 2022

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Aims To explore the function of phosphorylation of KAP1 (p-KAP1) at the serine-824 site (S824) in the proliferation and apoptosis of endogenous neural stem cells (NSCs) after cerebral ischemic/reperfusion (I/R). Methods The apoptosis and proliferation of C17.2 cells transfected with the p-KAP1-expression plasmids and the expression of proliferation cell nuclear antigen (PCNA) and p-KAP1 were detected by immunofluorescence and Western blotting after the Oxygen Glucose deprivation/reperfusion model (OGD/R). The interaction of p-KAP1 and CUL4A with PCNA was analyzed by immunoprecipitation. In the rats MCAO model, we performed the adeno-associated virus (AAV) 2/9 gene delivery of p-KAP1 mutants to verify the proliferation of endogenous NSCs and the colocalization of PCNA and CUL4A by immunofluorescence. Results The level of p-KAP1 was significantly down-regulated in the stroke model in vivo and in vitro. Simulated p-KAP1(S824) significantly increased the proliferation of C17.2 cells and the expression of PCNA after OGD/R. Simulated p-KAP1(S824) enhanced the binding of p-KAP1 and PCNA and decreased the interaction between PCNA and CUL4A in C17.2 cells subjected to OGD/R. The AAV2/9-mediated p-KAP1(S824) increased endogenous NSCs proliferation, PCNA expression, p-KAP1 binding to PCNA, and improved neurological function in the rat MCAO model. Conclusions Our findings confirmed that simulated p-KAP1(S824) improved the survival and proliferation of endogenous NSCs. The underlying mechanism is that highly expressed p-KAP1(S824) promotes binding to PCNA, and inhibits the binding of CUL4A to PCNA. This reduced CUL4A-mediated ubiquitination degradation to increase the stability of PCNA and promote the survival and proliferation of NSCs.

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Section: Discussionmentioning

confidence: 99%

KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA

et al. 2022

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“…Lately, the utility of plant-derived EVs in therapeutic drugs has also gained substantial interest ( 23 , 24 ). Hence, we intended to isolate EVs from M. charantia that its extract polysaccharide had neuroprotective effects against cerebral ischemia/reperfusion injury via scavenging excessive free radicals ( 9 ) and enhancing neural stem cells proliferation ( 10 ) and differentiation ( 11 ). Employing desentity gradient centrifugation, we successfully obtained MCELNs with typical sizes, shapes, and markers (TSG101, CD63, and CD9) identified to EVs.…”

Section: Discussionmentioning

confidence: 99%

“…We found that M. charantia extracts polysaccharides protected against cerebral ischemia/reperfusion injury via suppressing oxidative stress-mediated c-Jun N-terminal Kinase 3 signaling pathway ( 9 ). In addition, M. charantia polysaccharide promoted neural stem cells proliferation and differentiation via the SIRT1/Beta-catenin axis ( 10 , 11 ). Considering the ROS scavenging activity of M. charantia polysaccharides, we wonder about its radioprotective capacity against RIHD.…”

Section: Introductionmentioning

confidence: 99%

Momordica. charantia-Derived Extracellular Vesicles-Like Nanovesicles Protect Cardiomyocytes Against Radiation Injury via Attenuating DNA Damage and Mitochondria Dysfunction

Cui

Wang

et al. 2022

Front. Cardiovasc. Med.

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Thoracic radiotherapy patients have higher risks of developing radiation-induced heart disease (RIHD). Ionizing radiation generates excessive reactive oxygens species (ROS) causing oxidative stress, while Momordica. charantia and its extract have antioxidant activity. Plant-derived extracellular vesicles (EVs) is emerging as novel therapeutic agent. Therefore, we explored the protective effects of Momordica. charantia-derived EVs-like nanovesicles (MCELNs) against RIHD. Using density gradient centrifugation, we successfully isolated MCELNs with similar shape, size, and markers as EVs. Confocal imaging revealed that rat cardiomyocytes H9C2 cells internalized PKH67 labeled MCELNs time-dependently. In vitro assay identified that MCELNs promoted cell proliferation, suppressed cell apoptosis, and alleviated the DNA damage in irradiated (16 Gy, X-ray) H9C2 cells. Moreover, elevated mitochondria ROS in irradiated H9C2 cells were scavenged by MCELNs, protecting mitochondria function with re-balanced mitochondria membrane potential. Furthermore, the phosphorylation of ROS-related proteins was recovered with increased ratios of p-AKT/AKT and p-ERK/ERK in MCELNs treated irradiated H9C2 cells. Last, intraperitoneal administration of MCELNs mitigated myocardial injury and fibrosis in a thoracic radiation mice model. Our data demonstrated the potential protective effects of MCELNs against RIHD. The MCELNs shed light on preventive regime development for radiation-related toxicity.

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“…The results showed that Lactobacillus plantarum 69-2 and GOS could restore hepatic antioxidant activity and alleviate aging by activating the hepatic adenosine monophosphate-activated protein kinase (AMPK)/Sirt1 signal pathway. For instance, bitter melon polysaccharide could activate Sirt1-mediated β -linked protein deacetylation to exert neuroprotective effects [ 73 , 74 ]. Lentinan (LEN) has various biological properties such as anticancer, antibacterial, antiviral, and antioxidant effects and could protect cardiomyocytes from hypoxic damage by regulating microRNA-22/Sirt1 [ 75 ].…”

Section: Polysaccharides and Aging-related Signal Pathwaysmentioning

confidence: 99%

Antiaging Effects of Dietary Polysaccharides: Advance and Mechanisms

Han

Huang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Aging is a process in which the various physiological functions of the body gradually deteriorate and eventually lead to death. During this process, the body’s resistance to external stresses gradually decreases and the aging-related diseases gradually are increased. Polysaccharides are a group of active substances extracted from living organisms and are widely found in plants, animals, and microorganisms. In the last decade, a variety of natural polysaccharides from functional and medicinal foods have attracted considerable interest for their beneficial effects in the prevention of chronic diseases such as cancers, diabetes, and neurodegenerative diseases. Interestingly, these polysaccharides have also been found to delay aging by reducing oxidative damage, inhibiting telomere shortening, and being anti-inflammatory in different animal models of aging. These reviews summarized the progresses in effects of polysaccharides on antiaging and the potential mechanisms and especially focused on the signaling pathways involved in the antiaging functions. Finally, the applications and prospects of the antiaging effects of polysaccharides are discussed.

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Promotion of Momordica Charantia polysaccharides on neural stem cell proliferation by increasing SIRT1 activity after cerebral ischemia/reperfusion in rats

Cited by 27 publications

References 61 publications

KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA

KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA

Momordica. charantia-Derived Extracellular Vesicles-Like Nanovesicles Protect Cardiomyocytes Against Radiation Injury via Attenuating DNA Damage and Mitochondria Dysfunction

Antiaging Effects of Dietary Polysaccharides: Advance and Mechanisms

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