Promotion of neurite outgrowth and protective effect of erythropoietin on the retinal neurons of rats

Zhong, Yisheng; Yao, Huiping; Deng, Lianfu; Cheng, Yu; Zhou, Xiaoqing

doi:10.1007/s00417-007-0671-9

Cited by 30 publications

(14 citation statements)

References 38 publications

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“…In cells that involve the brain or the retina, EPO can prevent injury from hypoxic ischemia (Chong et al, 2002b;Liu et al, 2006;Meloni et al, 2006;Wei et al, 2006;Yu et al, 2005), excitotoxicity (Montero et al, 2007;Yamasaki et al, 2005), infection (Kaiser et al, 2006), free radical exposure Chong et al, 2003e;Yamasaki et al, 2005), staurosporine (Pregi et al, 2006), and dopaminergic cell injury (Demers et al, 2005;McLeod et al, 2006;Signore et al, 2006). In addition, administration of EPO also represents a viable option for the prevention of retinal cell injury during glutamate toxicity (Zhong et al, 2007) and glaucoma (Tsai et al, 2007). Systemic application of EPO also can improve functional outcome and reduce cell loss during spinal cord injury (King et al, 2007;Okutan et al, 2007), traumatic cerebral edema (Verdonck et al, 2007), cortical trauma (Cherian et al, 2007), and epileptic activity (Mikati et al, 2007;Nadam et al, 2007).…”

Section: Epo and Neurodegenerationmentioning

confidence: 99%

Erythropoietin: Elucidating new cellular targets that broaden therapeutic strategies

Maiese

Chong

et al. 2008

Progress in Neurobiology

102

155

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Given that erythropoietin (EPO) is no longer believed to have exclusive biological activity in the hematopoietic system, EPO is now considered to have applicability in a variety of nervous system disorders that can overlap with vascular disease, metabolic impairments, and immune system function. As a result, EPO may offer efficacy for a broad number of disorders that involve Alzheimer's disease, cardiac insufficiency, stroke, trauma, and diabetic complications. During a number of clinical conditions, EPO is robust and can prevent metabolic compromise, neuronal and vascular degeneration, and inflammatory cell activation. Yet, use of EPO is not without its considerations especially in light of frequent concerns that may compromise clinical care. Recent work has elucidated a number of novel cellular pathways governed by EPO that can open new avenues to avert deleterious effects of this agent and offer previously unrecognized perspectives for therapeutic strategies. Obtaining greater insight into the role of EPO in the nervous system and elucidating its unique cellular pathways may provide greater cellular viability not only in the nervous system but also throughout the body.

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Section: Epo and Neurodegenerationmentioning

confidence: 99%

Erythropoietin: Elucidating new cellular targets that broaden therapeutic strategies

Maiese

Chong

et al. 2008

Progress in Neurobiology

102

155

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“…Our previous studies demonstrated that EPO had a neuroprotective effect in vivo (15) and a neurite outgrowth promotion effect on retinal neurons in vitro (23). However, the mechanism of the axonal regeneration effect of EPO on retinal neurons has not been fully clarified.…”

Section: Discussionmentioning

confidence: 99%

“…The retinal explants in the control group were continually cultured with serum-free R16 nutrient medium; the retinal explants in the glutamate group were continually cultured with serum-free R16 nutrient medium containing 5 mM/l glutamate (Sigma-Aldrich, St. Louis, MO, USA); and the retinal explants in the glutamate + EPO group were continually cultured with serum-free R16 nutrient medium containing 5 mM/l glutamate and 6.0 U/ml EPO. The retinal explants in the three groups were continually cultured for another 72 h. The doses of glutamate and EPO used in the present study were selected according to our previous study in which it was shown that 6.0 U/ml EPO significantly improved the survival of cultured retinal neurocytes incubated with 5 mM/l glutamate (23).…”

Section: Methodsmentioning

confidence: 99%

The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate

Huang

Wang

Shen

et al. 2012

Molecular Medicine Reports

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Abstract. The aim of this study was to investigate the effects of erythropoietin (EPO) on RhoA/Rho-associated kinase (ROCK) expression in rat retinal explants cultured with glutamate. After the retinal explants were cultured in serumfree R16 nutrient medium for 24 h, the retinal explants were divided into control (R16 nutrient medium), glutamate (R16 nutrient medium containing 5 mM/l glutamate) and glutamate + EPO (R16 nutrient medium containing 5 mM/l glutamate and 6.0 U/ml EPO) groups, and culturing was continued for another 72 h. The mRNA and protein expression of total RhoA, ROCK1 and ROCK2 in the retinal explants was examined by RT-PCR and western blotting, and active RhoA in the retinal explants was detected via GST-RBD binding and immunoblotting with an antibody specific to active RhoA. The total RhoA mRNA and protein expression did not differ substantially between the control, the glutamate and the glutamate + EPO groups. The glutamate increased the active RhoA, ROCK1 and ROCK2 expression in cultured retinal explants (P<0.05), whereas the expression of active RhoA, ROCK1 and ROCK2 in the glutamate + EPO group was significantly lower than that in the simple glutamate group (P<0.05) and similar to that in the control group. In conclusion, EPO downregulates active RhoA, ROCK1 and ROCK2 expression in retinal explants cultured with glutamate.

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“…In cells that involve the brain or the retina, EPO can prevent injury from hypoxic ischemia [45,110,[225][226][227], excitotoxicity [228,229], infection [230], free radical exposure [61, 105,229], amyloid exposure [112], staurosporine [231], and dopaminergic cell injury [232]. In addition, administration of EPO also represents a viable option for the prevention of retinal cell injury during glutamate toxicity [233] and glaucoma [234]. Systemic application of EPO also can improve functional outcome and reduce cell loss during spinal cord injury [235,236], traumatic cerebral edema [237], cortical trauma [238], and epileptic activity [211,239].…”

Section: Erythropoietin a Cytokine And Growth Factormentioning

confidence: 99%

Triple play: Promoting neurovascular longevity with nicotinamide, WNT, and erythropoietin in diabetes mellitus

Maiese

2008

Biomedicine & Pharmacotherapy

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SummaryOxidative stress is a principal pathway for the dysfunction and ultimate destruction of cells in the neuronal and vascular systems for several disease entities, non promoting the ravages of oxidative stress to any less of a degree than diabetes mellitus. Diabetes mellitus is increasing in incidence as a result of changes in human behavior that relate to diet and daily exercise and is predicted to affect almost 400 million individuals worldwide in another two decades. Furthermore, both type 1 and type 2 diabetes mellitus can lead to significant disability in the nervous and cardiovascular systems, such as cognitive loss and cardiac insufficiency. As a result, innovative strategies that directly target oxidative stress to preserve neuronal and vascular longevity could offer viable therapeutic options to diabetic patients in addition to more conventional treatments that are designed to control serum glucose levels. Here we discuss the novel application of nicotinamide, Wnt signaling, and erythropoietin that modulate cellular oxidative stress and offer significant promise for the prevention of diabetic complications in the nervous and vascular systems. Essential to this process is the precise focus upon diverse as well as common cellular pathways governed by nicotinamide, Wnt signaling, and erythropoietin to outline not only the potential benefits, but also the challenges and possible detriments of these therapies. In this way, new avenues of investigation can hopefully bypass toxic complications, or at the very least, avoid contraindications that may limit care and offer both safe and robust clinical treatment for patients.

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Promotion of neurite outgrowth and protective effect of erythropoietin on the retinal neurons of rats

Abstract: EPO had a significant biological effect on neurite outgrowth of the dissociated retinal neurocytes in vitro. EPO was beneficial in promoting the survival and decreasing the apoptosis rates of the cultured retinal neurocytes suffering from glutamate-induced cytotoxicity.

Cited by 30 publications

References 38 publications

Erythropoietin: Elucidating new cellular targets that broaden therapeutic strategies

Erythropoietin: Elucidating new cellular targets that broaden therapeutic strategies

The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate

Triple play: Promoting neurovascular longevity with nicotinamide, WNT, and erythropoietin in diabetes mellitus

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