Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair

Li, Xiaoran; Xiao, Zhifeng; Han, Jin Soo; Chen, Lei; Xiao, Huahua; Ma, Fukai; Hou, Xun; Li, Xing; Sun, Jie; Ding, Wenyong; Zhao, Yannan; Chen, Bing; Dai, Jianwu

doi:10.1016/j.biomaterials.2013.03.062

Cited by 105 publications

(64 citation statements)

References 30 publications

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“…In our previous study, the NeuroRegen scaffold was prepared from bovine aponeurosis, which mainly consists of linearly ordered collagen fibers. When the scaffold was transplanted into complete SCI rat and canine models, it successfully guided neurite outgrowth along its fibers and decreased scar formation (Fan et al, 2010;Han et al, 2009;Han et al, 2010;Han et al, 2015;Li et al, 2013;Lin et al, 2006). Based on these preclinical results, the clinical study was approved by the ethics committee and registered on the National Institute of Health database.…”

Section: Discussionmentioning

confidence: 99%

“…Our recent work showed that NeuroRegen scaffold with active factors increased neuronal differentiation of the transplanted or endogenous neural stem cells to produce different types of neurons throughout the lesion area. These newly generated relay neurons may further rebuild the synaptic connections with each other or with the host spinal neurons to improve locomotion outcomes in SCI animals Li et al, 2013). Thus, rebuilding neuronal relays at the injury site would be a more efficient way to repair SCI compared with inducing axonal regrowth (Xiao et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The NeuroRegen scaffold bridges the gap of the lesion and acts as a vehicle to deliver stem cells or biomolecules to promote neural regeneration. In our previous study, a functional biomaterial consisting of NeuroRegen scaffold, biologically active molecules (neurotrophic factors or antagonists to myelin-associated inhibitors), and stem cells was developed that greatly promoted neural regeneration and functional recovery in SCI animals (Han et al, 2010;Han et al, 2015;Li et al, 2015;Li et al, 2013). In future work, biologically active molecules or stem cells could be added to the NeuroRegen scaffold for clinical SCI repair.…”

Section: Discussionmentioning

confidence: 99%

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One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients

Xiao

Tang

et al. 2016

Sci. China Life Sci.

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The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, NeuroRegen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemical barrier that prevents neural regeneration. However, identification of scar tissue is still a major challenge. In this study, the nerve electrophysiology method was used to distinguish scar tissue from normal neural tissue, and then different lengths of scars ranging from 0.5-4.5 cm were surgically resected in five complete chronic spinal cord injury (SCI) patients. The NeuroRegen scaffold along with autologous bone marrow mononuclear cells (BMMCs), which have been proven to promote neural regeneration and SCI recovery in animal models, were transplanted into the gap in the spinal cord following scar tissue resection. No obvious adverse effects related to scar resection or NeuroRegen scaffold transplantation were observed immediately after surgery or at the 12-month follow-up. In addition, patients showed partially autonomic nervous function improvement, and the recovery of somatosensory evoked potentials (SSEP) from the lower limbs was also detected. The results indicate that scar resection and NeuroRegen scaffold transplantation could be a promising clinical approach to treating SCI.NeuroRegen scaffold, chronic spinal cord injury, scar resection, collagen scaffold transplantation, bone marrow mononuclear cells, tissue regeneration Citation:Xiao, Z., Tang, F., Tang, J., Yang, H., Zhao, Y., Chen, B., Han, S., Wang, N., Li, X., Cheng, S., Han, G., Zhao, C., Yang, X., Chen, Y., Shi, Q., Hou, S., Zhang, S., and Dai, J. (2016). One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients. Sci China Life Sci 59, 647 -655.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients

Xiao

Tang

et al. 2016

Sci. China Life Sci.

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“…EGF has positive effects on proliferation and/or differentiation of neurons (24). Activated EGF receptor (EGFR) by EGF reduces the cytotoxic effect of TNF-α in some brain tumors (25).…”

Section: +mentioning

confidence: 99%

Investigation of Epidermal Growth Factor, Tumor Necrosis Factor-alpha and Thioredoxin System in Rats Exposed to Cerebral Ischemia

Erol-Demirbilek

Kılıç

Kömürcü

2016

Revista Romana De Medicina De Laborator

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“…instead, it is combined with other supportive materials to enhance growth promotion [35,39,52,53] and cell differentiation [54] . Recently, it was shown that the [86] iHC [31,32,38] Bladder function [31,32] BBB [87] inclined plane [87] Walking movements [40] Reflexive behaviors [40] Climbing ability [40] Electrophysiology [40] BBB [49,88] Combined behavior score [88] iHC [49,88] Robotic recording [88] Von Frey filaments [89] iHC [62,[89][90][91] Horizontal ladder [91] Beamwalk [91] BBB [70,71,92] inclined plane [70] Von Frey [70] iHC [70,92,93] Electrophysiology [70,72] Structural MRi [70] BBB [85,94,95] iHC [85,94,95] Electrophysiology …”

Section: Polymersmentioning

confidence: 99%

Biomaterials for spinal cord repair

Haggerty

Oudega

2013

Neurosci. Bull.

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Spinal cord injury (SCi) results in permanent loss of function leading to often devastating personal, economic and social problems. A contributing factor to the permanence of SCi is that damaged axons do not regenerate, which prevents the re-establishment of axonal circuits involved in function. Many groups are working to develop treatments that address the lack of axon regeneration after SCi. The emergence of biomaterials for regeneration and increased collaboration between engineers, basic and translational scientists, and clinicians hold promise for the development of effective therapies for SCi. A plethora of biomaterials is available and has been tested in various models of SCi. Considering the clinical relevance of contusion injuries, we primarily focus on polymers that meet the specific criteria for addressing this type of injury. Biomaterials may provide structural support and/or serve as a delivery vehicle for factors to arrest growth inhibition and promote axonal growth. Designing materials to address the specific needs of the damaged central nervous system is crucial and possible with current technology. Here, we review the most prominent materials, their optimal characteristics, and their potential roles in repairing and regenerating damaged axons following SCi.Keywords: spinal cord injury; axon regeneration; biodegradable materials; extracellular matrix proteins; functional recovery; growth factor; guidance; injury and repair; spinal motor neuron IntroductionSpinal cord injury (SCi) causes loss of neurons and axons resulting in motor and sensory function impairments. There are thousands of new cases of SCi in the world annually [1,2] occurring often in young adults. The lack of endogenous repair and the significant costs to the individual, family and society [1] are important motivations behind the continuing efforts to develop effective therapies. Promoting axonal regeneration is considered a potential repair strategy because it could lead to recovery of axonal circuits involved in motor and/or sensory function [3] .The central nervous system (CNS) neurons are intrinsically capable of some regeneration of damaged axons [4] but their attempts after SCi are frustrated by structural and chemical obstructions in the damaged nervous tissue [5] . Spinal cord repair approaches typically lead to small functional gains. one feature that most of these approaches have in common is a poor axonal regeneration response, suggesting that promoting axonal regeneration, including synapse formation, is essential to achieve substantial functional repair after SCi. if so, it is rational to anticipate that effective spinal cord therapies will need to include interventions addressing the axon growth-inhibition that leads to the poor axonal growth responses. As introduced above, axonal regeneration is considered an important repair mechanism for the injured spinal cord. Therefore, this review focuses on materials that have shown most promise to elicit an axonal regeneration response to foster functional restoration after ...

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Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair

Cited by 105 publications

References 30 publications

One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients

One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients

Investigation of Epidermal Growth Factor, Tumor Necrosis Factor-alpha and Thioredoxin System in Rats Exposed to Cerebral Ischemia

Biomaterials for spinal cord repair

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