Promotion of Ovarian Follicular Development by Injecting Vascular Endothelial Growth Factor (VEGF) and Growth Differentiation Factor 9 (GDF-9) Genes

Shimizu, Takashi

doi:10.1262/jrd.17072

Cited by 36 publications

(26 citation statements)

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“…One of the main properties of VEGF is prevention of apoptosis and inflammation (Jung Won Jeona et al 2014). Results of the present research are similar to those of a previous study that has demonstrated VEGF injection to the ovary caused angiogenesis, which reduces ischemia and prevents apoptosis (Shimizu 2006). In this research, a higher dosage of VEGF was shown to be the most effective to induce angiogenesis and to prevent apoptosis in transplanted tissue.…”

Section: Discussionsupporting

confidence: 89%

“…VEGF as an angiogenic factor decreases ischemia and prolongs the graft functionality that helps to keep normal follicular population in whole transplanted mouse ovary. Results of this research confirm previous researches about positive effects of VEGF on function transplanted ovaries (Shimizu 2006;Labied et al 2013). However, the effect of VEGF on transplantation of whole mouse ovary on gluteal muscle was evaluated.…”

Section: Discussionsupporting

confidence: 87%

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Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Zand-vakili

Eimani

Golkar‐Narenji

et al. 2016

Animal Cells and Systems

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One of the most important factors affecting survival rate of ovarian follicles during transplantation period is proper vascular development. The objective of the study was to evaluate the effect of vascular endothelial growth factor (VEGF) on auto-transplanted ovarian tissue. Twenty-one-dayold female mice (n = 30) transplanted as control group and 21-day-old female mice (n = 40) were divided into 4 groups that were treated with 0.5, 1, 2 and 4 µg/mL of VEGF directly injected to auto-transplanted ovarian tissue. Twenty-one days after transplantation, mice were treated with 7.5 IU pregnant mare serum gonadotropin and human chorionic gonadotropin. Transplanted ovaries were removed and sections were prepared from transplanted tissues for staining. The most effective dosage of VEGF on transplanted tissue was determined over H&E (hematoxylin and eosin) staining results. Slides were compared using TUNEL staining and CD31 assay for the most effective dosage. The percentages of preantral and antral follicles were not significantly different between transplanted group with 4 µg/mL VEGF and non-transplanted group. Lower apoptotic areas and higher CD31 expression were observed in transplanted ovaries treated with 4 µg/mL VEGF when compared to transplanted ovaries without VEGF treatment. VEGF positively affects the quality of ovarian tissue during transplantation. Survival rate of follicles and follicular development has improved with the effect of VEGF. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 87%

Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Zand-vakili

Eimani

Golkar‐Narenji

et al. 2016

Animal Cells and Systems

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“…The importance of VEGFA, KDR, and FLT1 in biological processes such as embryogenesis (Carmeliet et al 1996), folliculogenesis (Shimizu 2006), and seminiferous cord formation in the embryonic testis (Bott et al 2006) has been demonstrated. An increasing number of reports suggest that VEGFA has direct effects on nonvascular cells including neuronal cells (Marti & Risau 1998, Wada et al 2006, Nishijima et al 2007), muscle cells (Germani et al 2003, Bryan et al 2008, and granulosa cells (Greenaway et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Vascular endothelial growth factor regulates germ cell survival during establishment of spermatogenesis in the bovine testis

Caires

Avila²,

McLean

2009

Reproduction

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Vascular endothelial growth factor-A (VEGFA) is a hypoxia-inducible peptide essential for angiogenesis and targets nonvascular cells in a variety of tissues and cell types. The objective of the current study was to determine the function of VEGF during testis development in bulls. We used an explant tissue culture and treatment approach to test the hypothesis that VEGFA-164 could regulate the biological activity of bovine germ cells. We demonstrate that VEGFA, KDR, and FLT1 proteins are expressed in germ and somatic cells in the bovine testis. Treatment of bovine testis tissue with VEGFA in vitro resulted in significantly more germ cells following 5 days of culture when compared with controls. Quantitative real-time RT-PCR analysis determined that VEGF treatment stimulated an intracellular response that prevents germ cell death in bovine testis tissue explants, as indicated by increased expression of BCL2 relative to BAX and decreased expression of BNIP3 at 3, 6, and 24 h during culture. Blocking VEGF activity in vitro using antisera against KDR and VEGF significantly reduced the number of germ cells in VEGF-treated testis tissue to control levels at 120 h. Testis grafting provided in vivo evidence that bovine testis tissue treated with VEGFA for 5 days in culture contained significantly more differentiating germ cells compared with controls. These findings support the conclusion that VEGF supports germ cell survival and sperm production in bulls.

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“…In particular, the vascular endothelial growth factor A (VEGFA) seems to play a major role in ovarian angiogenesis as demonstrated recently by several pieces of evidence (Wulff et al 2001, Zimmermann et al 2003, Kaczmarek et al 2005, Shimizu & Sato 2005, Abramovich et al 2006, Shimizu 2006. Even if relevant progress has been made in understanding the mechanisms involved in the angiogenic events during the dominant final phase of tertiary follicle development (Fraser 2006, Martelli et al 2006, the role of blood vessel remodelling in early follicular stages is unclear.…”

Section: Introductionmentioning

confidence: 99%

Vascular supply as a discriminating factor for pig preantral follicle selection

Martelli¹,

Bernabò²,

Berardinelli³

et al. 2009

Reproduction

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This research analyses how somatic and vascular compartments change during preantral follicle growth. To address this aim, theca-granulosa (somatic) proliferation indexes (PIs), proportion of proliferating endothelial cells (PE), vascular area (VA) and vascular endothelial growth factor A (VEGFA) expression were simultaneously recorded on single healthy preantral follicles, classified into six different stages on the basis of the diameter and the granulosa layers. An autonomous blood vessel network starts to appear only in class 3. Vascular remodelling requires VEGFA expression, and VEGFA mRNA and VA significantly increase between class 3 and classes 4 and 5 and, further, in class 6. In addition, a positive correlation exists between these parameters in classes 3-5. Despite variation in angiogenesis results from classes 3 to 5, the statistical analysis reveals that the vascular parameters are positively and strictly correlated with somatic PIs. Conversely, class 6, also characterized by higher values of somatic PIs, displays a stable proportion of PEs (y40%) without showing any correlation among the different parameters analysed. To identify follicular subpopulations within different classes, a multivariate hierarchical cluster analysis was performed. This analysis reveals that the majority of classes 3 and 4 are quiescent follicles or structures that grow very slowly. Class 5 represents a transitory category, where half of the follicles maintain a low activity and the remaining express significantly higher levels of granulosa PI and VA. The follicles with this high activity are probably able to reach class 6 becoming dominant structures where somatic and vascular parameters are constantly on high levels and the VA remains the unique differentiating element.

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Promotion of Ovarian Follicular Development by Injecting Vascular Endothelial Growth Factor (VEGF) and Growth Differentiation Factor 9 (GDF-9) Genes

Abstract: Abstract. Ovarian follicular development in mammals is the complex process including endocrine, paracrine and autocrine.

Cited by 36 publications

References 50 publications

Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Vascular endothelial growth factor regulates germ cell survival during establishment of spermatogenesis in the bovine testis

Vascular supply as a discriminating factor for pig preantral follicle selection

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