Promotion of periostin expression contributes to the migration of Schwann cells

Sonnenberg-Riethmacher, Eva; Miehe, Michaela; Riethmacher, Dieter

doi:10.1242/jcs.174177

Cited by 20 publications

(24 citation statements)

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“…As a complementary approach, we sought to determine whether a HSC line can be induced to express POSTN. It has been reported that Neuregulin 1 (NRG1) and TGF-β can induce Postn expression in cultured rat Schwann cells (35). Further, Tgfb is highly expressed in the sciatic nerve of SAPP mice (Supplemental Figure 7), suggesting its presence at the site of inflammation.…”

Section: Resultsmentioning

confidence: 92%

“…PNS autoimmunity in NOD.Aire GW/+ mice shares key features with human CIDP, including infiltration of peripheral nerves by CD4 + T cells and F4/80 + macrophages (25)(26)(27), IFN-γ production by CD4 + T cells (28,29), and peripheral nerve demyelination (7,30). We screened mRNA levels of 6 ECM genes with known roles in PNS development: laminin a2 (Lama2) (31), laminin a4 (Lama4) (31), laminin a5 (Lama5) (32), laminin b1 (Lamb1) (31), thrombospondin 2 (Thbs2) (33), and Postn (34,35). We used quantitative reverse transcription PCR (qRT-PCR) to compare the relative expression levels of these genes between sciatic nerves from age-matched WT (NOD.…”

Section: Resultsmentioning

confidence: 99%

“…Postn is upregulated in various diseases and injuries (59) including cancer (60), myocardial infarction (61), asthma (62), skin inflammation (21), glomerulonephritis (22), and colitis (43). TGF-β or neuregulin/ERBB3 signaling (35,63) can induce Postn expression in Schwann cells during development; additionally, Postn expression can also be induced by IL-4 and IL-13 in keratinocytes during allergic skin inflammation (21) and by TNF-α and IL-17 during liver fibrosis (64). POSTN signals through ITGAV/ B3 (21,42), ITGAV/B5 (42), and ITGAM (eosinophils) (41) and results in the phosphorylation of focal adhesion kinase (FAK) (65) and AKT (65) in neurons and the activation of NF-κB (21,43) in keratinocytes and colon.…”

Section: Discussionmentioning

confidence: 99%

“…*P < 0.05 and **P < 0.005, by log-rank test (A), 2-tailed, unpaired t test with Welch's correction (C-E and G), or Fisher's exact test (J). jci.org Volume 128 Number 10 October 2018Postn is expressed in the PNS during embryonic development, it has been reported that Postn-deficient mice have normal PNS function(35) demonstrating that Postn is dispensable for PNS development. Consistent with this, we saw no overt signs of PNS impairment in NOD.Postn -/mice when assessed clinically (SupplementalFigure 8A), electrophysiologically (SupplementalFigure 8, B-E), or anatomically (SupplementalFigure 8, F-H).…”

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confidence: 99%

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Schwann cell–derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment

Allard¹,

Wang²,

Li³

et al. 2018

Journal of Clinical Investigation

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Chronic inflammatory demyelinating polyneuropathy (CIDP) and Guillain-Barre syndrome (GBS) are inflammatory neuropathies that affect humans and are characterized by peripheral nerve myelin destruction and macrophage-containing immune infiltrates. In contrast to the traditional view that the peripheral nerve is simply the target of autoimmunity, we report here that peripheral nerve Schwann cells exacerbate the autoimmune process through extracellular matrix (ECM) protein induction. In a spontaneous autoimmune peripheral polyneuropathy (SAPP) mouse model of inflammatory neuropathy and CIDP nerve biopsies, the ECM protein periostin (POSTN) was upregulated in affected sciatic nerves and was primarily expressed by Schwann cells. Postn deficiency delayed the onset and reduced the extent of neuropathy, as well as decreased the number of macrophages infiltrating the sciatic nerve. In an in vitro assay, POSTN promoted macrophage chemotaxis in an integrin-AM (ITGAM) and ITGAV-dependent manner. The PNS-infiltrating macrophages in SAPP-affected nerves were pathogenic, since depletion of macrophages protected against the development of neuropathy. Our findings show that Schwann cells promote macrophage infiltration by upregulating Postn and suggest that POSTN is a novel target for the treatment of macrophage-associated inflammatory neuropathies.

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Schwann cell–derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment

Allard¹,

Wang²,

Li³

et al. 2018

Journal of Clinical Investigation

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“…We demonstrated that the competent human EGFR + SCp can be readily isolated from 365 differentiated hPSCs and form de novo myelination both in vitro and in vivo. The EGFR + cells 366 isolated from our in vitro system had unique gene expression profile including several genes and 367 pathways that have prominent roles in different steps of SC development and myelination 368 (Campana et al, 2003;Tawk et al, 2011) (Sonnenberg-Riethmacher et al, 2015. Importantly, 369 these cells are expandable and cryopreserved cells reserved their myelin forming ability when 370 co-cultured with peripheral neurons and in vivo.…”

Section: Discussion 359mentioning

confidence: 97%

Modeling Human Peripheral Myelin Fully from Pluripotent Stem Cells and Immune-mediated NeuropathyIn Vitrousing ZIKA Virus

Mirakhori

Qin

2020

Preprint

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11The generation of in vitro model of human peripheral myelin development and associated 12 disease from human pluripotent stem cells (hPSCs) has been a challenge so far. In addition, the 13 underlying mechanism for ZIKA virus (ZIKV) infection incurred Guillain-Barré syndrome 14 (GBS) remains unexplored due to the lack of a suitable model. Here, we report the de novo 15 generation of a human peripheral myelination model with competent Schwann cells (SCs). 16Those human SCs generated from hPSCs via compound screening were capable of forming 17 compact myelin both in vitro and in vivo. We found ZIKV infection caused GBS-like events in 18 vitro including myelin sheath degeneration, as well as dysregulated transcriptional profile 19 including the activated cell death pathways and cytokine production. These effects could be 20 partially reversed by several pharmacological inhibitors. Our model therefore provides a new and 21 robust tool for studying the pathogenic mechanisms and developing of therapeutic strategies for 22 related neuropathies. 23 24 25

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Neuregulin‐1 promotes the proliferation, migration, and angiogenesis of human periodontal ligament stem cells in vitro

Shang

Kang

et al. 2022

Cell Biology International

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Neuregulin-1 (NRG-1) can promote the proliferation, migration, and angiogenesis of multiple stem cells, as well as prohibit cell apoptosis. In the present study, we aimed to explore the effects of NRG-1 on the proliferation, migration, apoptosis, angiogenic, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. The expression of erythroblastic leukemia viral oncogene homolog 2 (ERBB2), ERBB3, and ERBB4 on PDLSCs were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and immunofluorescence. The effects of NRG-1 on the proliferation, migration, apoptosis, angiogenic and osteogenic differentiation of PDLSCs were assessed by cell proliferation and viability assays, transwell migration assay, flow cytometry assay, tubule formation assay, alkaline phosphatase (ALP) activity, and Alizarin Red S staining, respectively. Gene expression of angiogenesis and osteogenesisrelated markers were detected by qRT-PCR. Among the ERBB family members, ERBB2 had the highest expression level in PDLSCs. Further, 10 ng/ml NRG-1 exhibited the maximal effect on proliferation, migration and remarkably inhibited the apoptosis of PDLSCs (p < .05). Moreover, NRG-1 upregulated the expression of vascular endothelial growth factor (VEGF), platelet/endothelial cell adhesion molecule-1 (CD31), hypoxia-inducible factor (HIF), kinase insert domain-containing receptor (KDR) in a dose-dependent manner as well as induced more tube formation. However, NRG-1 did not affect osteogenesis (p > .05). In summary, our study demonstrated that NRG-1 promotes the proliferation, migration, and angiogenesis and inhibits the apoptosis of PDLSCs in vitro and can potentially be used in tissue engineering for periodontal regeneration.

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Promotion of periostin expression contributes to the migration of Schwann cells

Cited by 20 publications

References 59 publications

Schwann cell–derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment

Schwann cell–derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment

Modeling Human Peripheral Myelin Fully from Pluripotent Stem Cells and Immune-mediated NeuropathyIn Vitrousing ZIKA Virus

Neuregulin‐1 promotes the proliferation, migration, and angiogenesis of human periodontal ligament stem cells in vitro

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