Promotion of skin fibroblasts collagen synthesis by polydioxanone mats combined with concentrated growth factor extracts

Abstract

The purpose of this study was to combine polydioxanone and concentrated growth factor extracts to enhance the stimulation of fibroblast collagen synthesis by polydioxanone in facial plastic. In this experiment, polydioxanone was prepared as a nanoscale bioresorbable scaffold by electrospinning. The PDO-CGF mat was prepared by loading the concentrated growth factor extract onto the polydioxanone mat by lyophilization after soaking. The diameter of the mat fibers was observed by scanning electron microscopy, and the mechanical properties, biocompatibility and cytotoxicity of the material were analyzed. The release rate of transforming growth factor-beat1 (TGF-β1) in the PDO-CGF mat was evaluated by enzyme-linked immunosorbent assay (ELISA), and the amount of collagen synthesis was evaluated by real-time polymerase chain reaction (RT-PCR) and immunofluorescence. The results showed that the PDO-CGF mat had a nanometer-scale fiber diameter and an elastic modulus similar to skin and was able to pull during cell contraction. The PDO-CGF mat showed good biocompatibility, and the appropriate concentration of the PDO-CGF mat can significantly promote cell proliferation. ELISA showed that the PDO-CGF mat stably and continuously released TGF-β1. RT-PCR and immunofluorescence staining showed that the PDO-CGF mat stimulated fibroblasts to synthesize type I and type III collagen. These results indicate that a suitable concentration of the PDO-CGF mat can better promote the synthesis of collagen by fibroblasts.

Optimization of a concentrated growth factor/mesoporous bioactive glass composite scaffold and its application in rabbit mandible defect regeneration

“…[59][60][61] It has been reported that freezedried CGFs in composite materials (loaded on chitosan-alginate composite gel or polydioxanone mat) can promote cell osteogenic induction 62 and the synthesis of collagen by fibroblasts. 63 Based on the above, a fdCGF/MBG composite scaffold was proposed and compared with the fibrin gel formation method in the rabbit mandibular defect model (Fig. 5B) by micro-CT scanning, VG staining, histological staining and sequential fluorescence staining.…”

Novel Applications of Concentrated Growth Factors in Facial Rejuvenation and Plastic Surgery

“…Yue et al 20 added 1%, 2%, 5% and 10% CGF to the culture medium of rat dermal fibroblasts, and found that cell proliferation was not proportional to CGF concentration. The results showed that 1% and 2% CGF could promote proliferation of rat dermal fibroblasts more significantly than other concentrations of CGF.…”

“…However, the cell growth and proliferation were not influenced. Yue et al 20 immersed PDO electrospun mats into medium containing CGF, and got the CGF-coated PDO mat through freeze drying. The results showed that 10% and 30% CGF-PDO mats could significantly stimulate proliferation of rat dermal fibroblasts, and promote the synthesis of Col I and Col III because of TGF-β1 released by CGF through raising the secretion of the enzyme collagenase 27 .…”

Considerations for Clinical Use of Concentrated Growth Factor in Maxillofacial Regenerative Medicine

“…Generally, multiple growth factors are collected from CGF extracts. 11 Platelet-growth factors are mostly represented by BMPs, transforming growth factor-b1, epidermal growth factor, PDGF, vascular endothelial growth factor, and basic-fibroblast growth factor. 12 In this systematic review, growth factors within CGF has been shown to stimulate the proliferation, migration and angiogenic or osteogenic differentiation of multiple stem cells in maxillofacial field, including dental pulp cells, 13,14 dental pulp stem cells, 15,16 stem cells of the apical papilla, 17,18 periodontal ligament cells, [19][20][21] periodontal ligament fibroblasts, 22 gingiva-derived mesenchymal stem cells, 23 bone marrow-derived stromal cells, 24,25 osteoblast cells, 26 and periosteum-derived cells.…”

“…Generally, multiple growth factors are collected from CGF extracts 11 . Platelet-growth factors are mostly represented by BMPs, transforming growth factor-β1, epidermal growth factor, PDGF, vascular endothelial growth factor, and basic-fibroblast growth factor 12 .…”