Promotion of survival and proliferation by interleukin 3, fcil‐ligand and erythropoietin on early and late appearing spleen colony forming units in culture

Abstract

Abstract. We examined the effects of interleukin 3 (IL-3), kit-ligand and erythropoietin (EPO) on the survival and growth of early appearing spleen colony forming units (CFU-Ss) and late appearing CFU-S (CFU-SI2) in short-term liquid culture (SLC). In the control cultures, without any additive, CFU-S8 and CFU-SI2 declined; nearly 10% of the initial number of CFU-S still survived by the second day of culture. The addition of IL-3 or kit-ligand increased the survival both of CFU-S8 and CFU-S12, with an increased dose of concentration, a t final concentrations of 10-200 U/ml and 500-SOX dilution, respectively. However, only the survival of CFU-S8 increased when EPO was added up to 10 U/ml, while the frequency of CFU-Slz was not higher than in the control culture. The percentage of CFU-S8 and CFU-SIZ in DNA synthesis, evaluated in 'H-TdR cytocide experiments, increased after one day in culture with each of the three factors. The results suggest that all three factors stimulated the proliferation of both populations of CFU-S, but the two populations showed different patterns of response to each factor; EPO stimulates the proliferation of CFU-SIz that differentiate into CFU-S8 that have less capacity for self-renewal, while the addition of IL-3 or kit-ligand causes an increase in the number of both populations due to the stimulation of an earlier stage of stem cell differentiation or self-renewal of CFU-SI2. Our experimental system (SLC-CFU-S assay) is useful for evaluating the response of hematopoietic stem cells to cytokines which promote the in vitro survival and oroliferation of these cells.

Ability of Early Acting Cytokines to Directly Promote Survival and Suppress Apoptosis of Human Primitive CD34+CD38− Bone Marrow Cells With Multilineage Potential at the Single-Cell Level: Key Role of Thrombopoietin

“…The present findings on CD34 / CD38 0 BM progenitor and suppress apoptosis of primitive murine hematopoietic cells were in agreement with those previously made on canprogenitor cells, 4,6-14,37,49,50 less is known about the ability of didate murine stem cell populations, [8][9][10][11][12][13][14] in that IL-3, KL, and cytokines to promote viability of populations of candidate FL can directly promote viability of primitive progenitor human stem cells.…”

“…Address reprint requests to Ole J. Borge, MSc, Stem Cell Laborareconstituting stem cells, and that it can maintain the majority of such progenitor cells viable after prolonged culture. [11][12][13] Sweden. In contrast, studies of human CD34 / DR 0 BM progenitor The publication costs of this article were defrayed in part by page cells as well as CD34 / CD38 0 fetal liver cells have reported charge payment.…”

“…However, whereas KL has been found to be the most The present study is the first dissecting the ability of cytopotent viability-promoting cytokine for primitive murine kines to promote viability of human CD34 / CD38 0 BM proprogenitor cells and to maintain most progenitors viable genitor cells. CD34 / CD38 0 cells represent a minor fraction through prolonged culture, [11][12][13]37 KL (as well as IL-3 and of BM cells, but are highly enriched in primitive progenitor FL) were only capable of maintaining a low fraction of cells. [18][19][20] Thus, in addition to being used extensively for in CD34 / CD38 0 progenitor cells viable.…”

“…19,20 However, and for promote viability of subpopulations of primitive progenitor/ unknown reasons, effective growth of CD34 / CD38 0 cells stem cells. [6][7][8][9][10][11][12][13][14] Although less is known about putative viability occurs only in liquid medium and not in semisolid medium factors for human stem cells, IL-3, granulocyte-macrophage such as methylcellulose, which more selectively promotes colony-stimulating factor (GM-CSF), KL, and FL have been growth of more committed progenitor cells. 19,20,22 In addition shown to enhance viability of enriched primitive human proto being used extensively for in vitro studies of primitive genitor cell populations in culture.…”

Protective effects of cytokine combinations against the apoptotic activity of glucocorticoids on CD34+ hematopoietic stem/progenitor cells

“…The viability and proliferation of haematopoietic cells are strictly dependent on haematopoietic cytokines (Williams et al 1990), and haematopoietic cells die by apoptosis in the absence of cytokines, whereas malignant cells release cytokines in an autocrine manner (Kinoshita et al 1995;Lotem and Sachs 1999). Several studies have shown that cytokines promote viability and supress apoptosis in haematopoietic progenitor cells (Williams et al 1990;Ikebuchi et al 1988; Williams and Broxmeyer 1988;Bodine et al 1991;Itoh et al 1992;Katayama et al 1993;Sasaki et al 1993;Li and Johnson 1994;Jacobsen et al 1995;Keller et al 1995;Rasko et al 1995;Lotem and Sachs 1995;Metcalf 2008;Bordoni et al 2018). Cytokines can also protect cells from apoptotic death caused by chemotherapeutic agents and can decrease their toxic effects on haematopoietic cells (Griffin and Löwenberg 1986;Lotem and Sachs 1999;Zeuner et al 2003).…”