Promotion proliferation effect of a polysaccharide from Aloe barbadensis Miller on human fibroblasts in vitro

Yao, Hong; Chen, Yan; Li, Shaoguang; Huang, Liying; Chen, Wei; Lin, Xinhua

doi:10.1016/j.ijbiomac.2009.04.013

Cited by 29 publications

(20 citation statements)

References 24 publications

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“…Some species, especially A. vera L. are purported to have medicinal properties (Eriko et al, 2012;Woźnik and Paduch, 2012). Other uses of aloe include their role in alternative medicines and in home first aid (Yao et al, 2009;Dutta and Masakapalli, 2013). Both the translucent inner pulp as well as the resinous yellow exudates from the wounding aloe plant are used externally to relieve skin discomforts and internally as a laxative (Saini and Saini, 2011;He et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of silicon (Si) on growth, quality and ionic homeostasis of aloe under salt stress

Liu

2015

South African Journal of Botany

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The physiological characteristics of aloe irrigated with brackish water and the relationship with ameliorative effects of silicon (Si) nutrition were studied. Results of cultivation and analysis showed that 2.0 mmol · L −1 added Si significantly alleviated growth inhibition and quality reduction of aloe under 100 mmol · L −1 NaCl stress; added Si significantly reduced Na + content while increased K + content and K + /Na + ratio of salt stressed aloe plant, especially absorption and translocation selectivity of aloe root to K + and Na + were promoted, and ion homeostasis of aloe plant was kept well. Results of cross-sectional X-ray energy dispersive spectrum microanalysis of aloe root and leaf further confirmed the above findings. One of the mechanisms of silicon was its improvement to activity of plasma membrane H + -ATPase, tonoplast membrane H + -ATPase and H + -pyrophosphates of aloe root under salt stress.

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Section: Introductionmentioning

confidence: 99%

Effects of silicon (Si) on growth, quality and ionic homeostasis of aloe under salt stress

Liu

2015

South African Journal of Botany

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“…Polysaccharides extracted from various plants have been widely used for wound treatment in both traditional and modern medicine [30] . For example, polysaccharides from kiwi fruit or aloe have been reported to increase either the proliferation or the differentiation of keratinocytes and fibroblasts [30,31] . Concerning the dermatologic activities of lithospermic acid, the methanol extracted fraction of gromwell in which lithospermic acid and two derivative esters are isolated as main components, has been found to increase mRNA and protein expression of serine palmitoyl transferase in human keratinocyte HaCaT cells [32] .…”

Section: Discussionmentioning

confidence: 99%

Water Extract of Gromwell <i>(Lithospermum erythrorhizon)</i> Enhances Migration of Human Keratinocytes and Dermal Fibroblasts with Increased Lipid Synthesis in an in vitro Wound Scratch Model

Kim

Kim²,

Park³

et al. 2011

Skin Pharmacol Physiol

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Background: Although organic extracts of gromwell (Lithospermum erythrorhizon) have been shown to promote wound healing, the wound healing effects of water extracts of gromwell (WG) that are commonly used in traditional remedies have not been elucidated. Objective: We investigated whether WG promotes the migration and/or proliferation of cultured human keratinocytes (CHK) or dermal fibroblasts in parallel with increases in lipid synthesis during in vitro wound healing. Methods: CHK or fibroblasts were treated with 1–1,000 µg/ml WG for up to 48 h following scratch wound formation. Cell migration was assessed by measuring coverage (in percent) from the wound margin, while cell proliferation and lipid synthesis were determined by [³H]thymidine incorporation into DNA fractions, and [³H]palmitate or [³H]serine incorporation into lipid fractions, respectively. Results: Low-dose WG (1 µg/ml) enhanced the wound coverage for both CHK and fibroblasts at 24 h, while cell proliferation was not altered in either cell types. Synthesis of both total lipids and individual lipid classes, including phospholipids, sphingolipids and neutral lipids, were found to be increased at 24 h in CHK treated with 1 µg/ml WG; in similarly treated fibroblasts, only the syntheses of sphingolipids (such as ceramides and glucosylceramides), but not other lipid species, were significantly increased. In contrast, a higher dose of WG (10–1,000 µg/ml) did not enhance wound coverage, and 100 µg/ml WG neither altered cell proliferation nor lipid synthesis in both CHK and fibroblasts. Conclusion: Low-dose WG (1 µg/ml) enhances the migration of both CHK and fibroblasts with increased lipid synthesis in an in vitro wound scratch model.

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“…Dore et al [9] also found that the Sargassum vulgare polysaccharide increased viability of normal rabbit aortic endothelial cells to above 100% in the concentration range of 25-100 μg/mL and also promoted cell growth. Yao et al [10] reported that Aloe polysaccharide (API) speeded up the proliferation of fibroblasts significantly, which OD value detected by MTT was 0.48, 0.51, 0.55, 0.59, 0.63 and 0.65 at the concentration of 0, 25, 50, 100, 200 and 400 μg/mL.…”

Section: Repair Effect Of Six Low Molecular Polysaccharides With Diffmentioning

confidence: 99%

Degradation, Characterization, Ferrous Ion Chelating Ability and Repair Effect of Sulfated Polysaccharides of Six Kinds of Seaweed Polysaccharides

Sun¹,

Guo²,

Wang³

et al. 2018

Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

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Abstract. Six kinds of seaweed polysaccharides (SPSs) extracted from Laminaria japonica, Porphyra yezoensis, Gracilaria lemaneiformis, Sargassum fusiforme, Eucheuma gelatinae and Undaria pinnatifida were degraded and characterized. The sulfate group (-OSO 3 H) content of these polysaccharides were 21.7, 17.9, 13.3, 8.2, 7.0, and 5.5%, respectively. The ferrous ion chelating ability and repair effect on injured human kidney proximal tubular epithelial cells (HK-2) of these polysaccharides were studied. These polysaccharides had stronger ferrous ion chelating ability, suggesting that it possessed high antioxidant activity. Each polysaccharide had a repair effect on oxalate-induced damaged HK-2 cells. The antioxidant activity and repair ability of polysaccharide was positively correlated with its -OSO 3 H content. These results indicated that these polysaccharides, especially those with high -OSO 3 H content, may be potential drugs for the prevention and treatment of calcium oxalate stones.

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Promotion proliferation effect of a polysaccharide from Aloe barbadensis Miller on human fibroblasts in vitro

Cited by 29 publications

References 24 publications

Effects of silicon (Si) on growth, quality and ionic homeostasis of aloe under salt stress

Effects of silicon (Si) on growth, quality and ionic homeostasis of aloe under salt stress

Water Extract of Gromwell <i>(Lithospermum erythrorhizon)</i> Enhances Migration of Human Keratinocytes and Dermal Fibroblasts with Increased Lipid Synthesis in an in vitro Wound Scratch Model

Degradation, Characterization, Ferrous Ion Chelating Ability and Repair Effect of Sulfated Polysaccharides of Six Kinds of Seaweed Polysaccharides

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