Promotive effects of human induced pluripotent stem cell-conditioned medium on the proliferation and migration of dermal fibroblasts

Oh, Myeongsik; Kim, Yu Jin; Son, Young Ju; Yoo, Hyuk Sang; Park, Jae Young

doi:10.1007/s12257-017-0221-1

Cited by 16 publications

(14 citation statements)

References 47 publications

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“…The proliferation of HDFs was increased by iPSC-Exo in a dose-dependent manner up to 50 × 10 8 particles/mL and maintained with a higher concentration ( Figure 2 ). In our previous study, some toxicity had been shown at high concentrations of iPSC-CM [ 34 ]. However, no toxic effect was observed when iPSC-Exo was treated even with higher concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…It was supposed to be due to accumulation of toxic metabolites such as lactic acid and the depletion of nutrients in the iPSC-CM. For this reason, it was shown in the result of the MTT assay that the maximum increase in HDFs proliferation was limited, up to about 40% [ 34 ]. On the other hand, when iPSC-Exo was applied, the cell proliferation has increased by up to 80% without any significant cytotoxicity ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…We previously demonstrated the stimulatory effects of human induced pluripotent stem cell-conditioned medium (iPSC-CM) on the proliferation and migration of dermal fibroblasts [ 34 ]. Herein, we hypothesized that the iPSCs-CM contained exosomes and the human induced pluripotent stem cells-derived exosomes (iPSC-Exo) played a key role in these effects of iPSC-CM.…”

Section: Introductionmentioning

confidence: 99%

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Exosomes Derived from Human Induced Pluripotent Stem Cells Ameliorate the Aging of Skin Fibroblasts

Lee

Kim

et al. 2018

IJMS

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169

155

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Stem cells and their paracrine factors have emerged as a resource for regenerative medicine. Many studies have shown the beneficial effects of paracrine factors secreted from adult stem cells, such as exosomes, on skin aging. However, to date, few reports have demonstrated the use of exosomes derived from human pluripotent stem cells for the treatment of skin aging. In this study, we collected exosomes from the conditioned medium of human induced pluripotent stem cells (iPSCs) and investigated the effect on aged human dermal fibroblasts (HDFs). Cell proliferation and viability were determined by an MTT assay and cell migration capacity was shown by a scratch wound assay and a transwell migration assay. To induce photoaging and natural senescence, HDFs were irradiated by UVB (315 nm) and subcultured for over 30 passages, respectively. The expression level of certain mRNAs was evaluated by quantitative real-time PCR (qPCR). Senescence-associated-β-galactosidase (SA-β-Gal) activity was assessed as a marker of natural senescence. As a result, we found that exosomes derived from human iPSCs (iPSCs-Exo) stimulated the proliferation and migration of HDFs under normal conditions. Pretreatment with iPSCs-Exo inhibited the damages of HDFs and overexpression of matrix-degrading enzymes (MMP-1/3) caused by UVB irradiation. The iPSCs-Exo also increased the expression level of collagen type I in the photo-aged HDFs. In addition, we demonstrated that iPSCs-Exo significantly reduced the expression level of SA-β-Gal and MMP-1/3 and restored the collagen type I expression in senescent HDFs. Taken together, it is anticipated that these results suggest a therapeutic potential of iPSCs-Exo for the treatment of skin aging.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exosomes Derived from Human Induced Pluripotent Stem Cells Ameliorate the Aging of Skin Fibroblasts

Lee

Kim

et al. 2018

IJMS

Self Cite

169

155

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show abstract

“…The differentiation of stem cells is conventionally controlled by soluble cues; that is, the use of defined culture media containing multiple growth factors (e.g., bone morphogenetic proteins, insulin, peroxisome proliferator-activated receptor γ) and biomolecules (e.g., dexamethasone, ascorbic acid, glycerophosphate) [ 10 , 11 , 12 , 13 , 14 ]. However, recent findings suggest that biophysical cues, such as the physicochemical characteristics of the underlying substrate, to which the cells attach and actively interact with as they grow, can also play key roles in steering the transformation of MSCs into specific cell types [ 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells

Kang

Kim

Han

et al. 2018

IJMS

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Graphene derivatives have immense potential in stem cell research. Here, we report a three-dimensional graphene/arginine-glycine-aspartic acid (RGD) peptide nanoisland composite effective in guiding the osteogenesis of human adipose-derived mesenchymal stem cells (ADSCs). Amine-modified silica nanoparticles (SiNPs) were uniformly coated onto an indium tin oxide electrode (ITO), followed by graphene oxide (GO) encapsulation and electrochemical deposition of gold nanoparticles. A RGD–MAP–C peptide, with a triple-branched repeating RGD sequence and a terminal cysteine, was self-assembled onto the gold nanoparticles, generating the final three-dimensional graphene–RGD peptide nanoisland composite. We generated substrates with various gold nanoparticle–RGD peptide cluster densities, and found that the platform with the maximal number of clusters was most suitable for ADSC adhesion and spreading. Remarkably, the same platform was also highly efficient at guiding ADSC osteogenesis compared with other substrates, based on gene expression (alkaline phosphatase (ALP), runt-related transcription factor 2), enzyme activity (ALP), and calcium deposition. ADSCs induced to differentiate into osteoblasts showed higher calcium accumulations after 14–21 days than when grown on typical GO-SiNP complexes, suggesting that the platform can accelerate ADSC osteoblastic differentiation. The results demonstrate that a three-dimensional graphene–RGD peptide nanoisland composite can efficiently derive osteoblasts from mesenchymal stem cells.

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“…[ 4 , 5 ]. Moreover, ESCs are classified as pluripotent stem cells (PSCs) and have the ability to proliferate indefinitely and can generate any specific cell type in the body, such as endothelial cells, skin cells, and many others [ 6 , 7 , 8 ]. Additionally, it is possible to induce PSCs into multipotent cells, e.g., hematopoietic stem cells (HSCs), MSCs, NSCs, and so on [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Nanobiosensing Platforms for Real-Time and Non-Invasive Monitoring of Stem Cell Pluripotency and Differentiation

Suhito

Angeline

Choo

et al. 2018

Sensors

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Breakthroughs in the biomedical and regenerative therapy fields have led to the influential ability of stem cells to differentiate into specific types of cells that enable the replacement of injured tissues/organs in the human body. Non-destructive identification of stem cell differentiation is highly necessary to avoid losses of differentiated cells, because most of the techniques generally used as confirmation tools for the successful differentiation of stem cells can result in valuable cells becoming irrecoverable. Regarding this issue, recent studies reported that both Raman spectroscopy and electrochemical sensing possess excellent characteristics for monitoring the behavior of stem cells, including differentiation. In this review, we focus on numerous studies that have investigated the detection of stem cell pluripotency and differentiation in non-invasive and non-destructive manner, mainly by using the Raman and electrochemical methods. Through this review, we present information that could provide scientific or technical motivation to employ or further develop these two techniques for stem cell research and its application.

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Promotive effects of human induced pluripotent stem cell-conditioned medium on the proliferation and migration of dermal fibroblasts

Cited by 16 publications

References 47 publications

Exosomes Derived from Human Induced Pluripotent Stem Cells Ameliorate the Aging of Skin Fibroblasts

Exosomes Derived from Human Induced Pluripotent Stem Cells Ameliorate the Aging of Skin Fibroblasts

Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells

Nanobiosensing Platforms for Real-Time and Non-Invasive Monitoring of Stem Cell Pluripotency and Differentiation

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