Promotive Role of CircATRNL1 on Chondrogenic Differentiation of BMSCs Mediated by miR-338-3p

Zheng, Jianzhang; Lin, Yunshuo; Tang, Fa-Qiang; Guo, Hui-Ling; Yan, Laipeng; Hu, Shiping; Wu, Hong

doi:10.1016/j.arcmed.2021.02.003

Cited by 14 publications

(11 citation statements)

References 31 publications

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“…Articular cartilage damage is one of the main pathological changes in osteoarthritis, and cartilage repair is the key to solving osteoarthritis. Zheng et al ( 63 ) found that circATRNL1 (hsa_circ_0020093) was highly expressed during the chondrogenic differentiation of BMSCs, and also found that the chondrogenic differentiation-related factors SRY-related HMG box 9 (SOX9), type II collagen (COL2) and aggrecan were highly expressed. Overexpression of circATRNL1 enhanced the proliferation of BMSCs and simultaneously enhanced the expression of SOX9, COL2 and aggrecan, as well as the degree of chondrogenic differentiation of BMSCs, and miR-338-3p was its target ( 63 ).…”

Section: Associations Between Circrnas and Bmscsmentioning

confidence: 99%

“…Zheng et al ( 63 ) found that circATRNL1 (hsa_circ_0020093) was highly expressed during the chondrogenic differentiation of BMSCs, and also found that the chondrogenic differentiation-related factors SRY-related HMG box 9 (SOX9), type II collagen (COL2) and aggrecan were highly expressed. Overexpression of circATRNL1 enhanced the proliferation of BMSCs and simultaneously enhanced the expression of SOX9, COL2 and aggrecan, as well as the degree of chondrogenic differentiation of BMSCs, and miR-338-3p was its target ( 63 ). This study demonstrated that circATRNL1 promotes the cartilage differentiation of BMSCs by regulating miR-338-3p, which provides new insights into cartilage repair.…”

Section: Associations Between Circrnas and Bmscsmentioning

confidence: 99%

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Roles of circular RNAs in osteogenic differentiation of bone marrow mesenchymal stem cells (Review)

Wang

Zhang

et al. 2022

Mol Med Rep

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Bone marrow mesenchymal stem cells (BMSCs) can differentiate into osteoblasts, chondrocytes, adipocytes and even myoblasts, and are therefore defined as pluripotent cells. BMSCs have become extremely important seed cells in gene therapy, tissue engineering, cell replacement therapy and regenerative medicine due to their potential in multilineage differentiation, self-renewal, immune regulation and other fields. Circular RNAs (circRNAs) are a class of non-coding RNAs that are widely present in eukaryotic cells. Unlike standard linear RNAs, circRNAs form covalently closed continuous loops with no 5′ or 3′ polarity. circRNAs are abundantly expressed in cells and tissues, and are highly conserved and relatively stable during evolution. Numerous studies have shown that circRNAs play an important role in the osteogenic differentiation of BMSCs. Further studies on the role of circRNAs in the osteogenic differentiation of BMSCs can provide a new theoretical and experimental basis for bone tissue engineering and clinical treatment.

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Section: Associations Between Circrnas and Bmscsmentioning

confidence: 99%

Section: Associations Between Circrnas and Bmscsmentioning

confidence: 99%

Roles of circular RNAs in osteogenic differentiation of bone marrow mesenchymal stem cells (Review)

Wang

Zhang

et al. 2022

Mol Med Rep

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“…A previous clinical study revealed that the expression of miR-338-3p was increased in the serum of knee OA patients compared with normal controls and was positively correlated with visual analog scale (VAS) scores and joint space narrowing, indicating miR-338-3p may be as a promising biomarker for diagnosis of knee OA [ 46 ]. Furthermore, miR-338-3p in bone marrow mesenchymal stem cells (BMSCs) was reported to inhibit the differentiation of BMSCs into cartilage, characterized by decreased expression of SOX9, COL2, and Aggrecan [ 47 ]. However, in contradiction with these reports, our data demonstrated that miR-338-3p expression was decreased in IL-1β-stimulated chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

Exosomes derived from miR-338-3p-modified adipose stem cells inhibited inflammation injury of chondrocytes via targeting RUNX2 in osteoarthritis

et al. 2022

J Orthop Surg Res

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Background Osteoarthritis (OA) is a chronic degenerative disease that is one of the main causes of disability in middle-aged and elderly people. Adipose stem cell (ASC)-derived exosomes (ASC-Exo) could repair cartilage damage and treat OA. MiRNA-338-3p expression was confirmed to play a role in inhibiting proinflammatory cytokines. Herein, we aimed to explore the mechanism by which exosomes derived from miR-338-3p overexpressing ASCs protects chondrocytes from interleukin (IL)-1β-induced chondrocyte change. Methods Exosomes were extracted from ASCs transfected with miR-338-3p or its antisense inhibitor. The ASC-Exos (miR-338-3p silencing/overexpression) were incubated with IL-1β-induced ATDC5 cells, followed by evaluation of the chondrocyte proliferation, degradation, and inflammation injury. Results In vitro results revealed that ASC-Exos inhibited the expression of prostaglandin E2 (PGE2), IL-6, IL-1β, and TNF-α, as well as promoted the proliferation of ATDC5 cells. Moreover, ASC-Exos inhibited inflammation injury and degradation of ATDC5 cells by transferring miR-338-3p. Luciferase reporter assays showed that RUNX2 was a target gene of miR-338-3p. Additionally, RUNX2 overexpression in ATDC5 cells reversed the protective effect of miR-338-3p on chondrocytes. Taken together, this study demonstrated that exosomes secreted from miR-338-3p-modified ASCs were effective in the repair of IL-1β-induced chondrocyte change by inhibiting RUNX2 expression. Conclusions Our result provided valuable data for understanding the mechanism of ASC-Exos in OA treatment. Graphical abstract

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“…CircATRNL1 is the first reported coding circRNA in ovarian cancer. At present, seven studies have explored the roles of circATRNL1 ( Zhang et al, 2019a ; Wang et al, 2020a ; Chen et al, 2020 ; Wang et al, 2021a ; Wang et al, 2021b ; Zhang et al, 2021c ; Zheng et al, 2021 ), but three did not include has_circ_0020093 ( Chen et al, 2020 ; Wang et al, 2021b ; Zhang et al, 2021c ). In a study by Wang et al, circATRNL1 activated Smad4 signaling and suppressed angiogenesis and ovarian cancer metastasis by binding miR-378 ( Wang et al, 2021a ).…”

Section: Discussionmentioning

confidence: 99%

“…Zhang et al found that circATRNL1 was downregulated in women with polycystic ovary syndrome ( Zhang et al, 2019a ). Zheng et al showed that circATRNL1 is beneficial to bone marrow mesenchymal stem cell differentiation into cartilage by regulating miR-338-3p ( Zheng et al, 2021 ). However, circATRNL1 contributes to the progression of endometriosis by promoting epithelial–mesenchymal transition ( Wang et al, 2020a ).…”

Section: Discussionmentioning

confidence: 99%

CircATRNL1 and circZNF608 Inhibit Ovarian Cancer by Sequestering miR-152-5p and Encoding Protein

Lyu

Shen

et al. 2022

Front. Genet.

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Background: CircRNAs have been found to be involved in the pathogenesis of various diseases. We aimed to explore the roles of circRNAs in ovarian cancer.Methods: The expression levels of circRNAs in ovarian cancer and normal ovarian tissues were analyzed using RNA sequencing. Fluorescent in situ hybridization (FISH), proliferation assays and transwell assays were used to assess the effects of circRNAs on ovarian cancer.Results: CircATRNL1 and circZNF608 were downregulated in 20 ovarian cancer tissues compared to normal tissues. CircATRNL1 and circZNF608 are mainly located in the cytoplasm of ovarian cancer cells, and circATRNL1 is a highly conserved circRNA. The overexpression of circATRNL1 and circZNF608 inhibits the proliferation and invasion of ovarian cancer cells. We predicted miRNA–circRNA interactions for circZNF608 and circATRNL1 and obtained 63 interactions. However, a luciferase reporter assay showed that only miR-152-5p was sequestered by circZNF608. Bioinformatics analysis and experiments indicated that circATRNL1 contains an internal ribosome entry site and an open reading frame encoding a 131 aa protein.Conclusion: In conclusion, circATRNL1 and circZNF608 are two downregulated circRNAs in ovarian cancer and work as tumor suppressors. CircZNF608 may exert antitumor activity in ovarian cancer by binding miR-152-5p, and circATRNL1 may encode a 131 aa protein.

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Promotive Role of CircATRNL1 on Chondrogenic Differentiation of BMSCs Mediated by miR-338-3p

Cited by 14 publications

References 31 publications

Roles of circular RNAs in osteogenic differentiation of bone marrow mesenchymal stem cells (Review)

Roles of circular RNAs in osteogenic differentiation of bone marrow mesenchymal stem cells (Review)

Exosomes derived from miR-338-3p-modified adipose stem cells inhibited inflammation injury of chondrocytes via targeting RUNX2 in osteoarthritis

CircATRNL1 and circZNF608 Inhibit Ovarian Cancer by Sequestering miR-152-5p and Encoding Protein

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