Pronuclear relationship and association of maternal and paternal chromosomes in flushed rabbit ova

Gondos, Bernard; Bhiraleus, Prapas

doi:10.1007/bf00339782

Cited by 17 publications

(7 citation statements)

References 16 publications

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“…Similar to data observed in the rabbit (Gondos and Bhiraleus, 1970) and man (Van Blerkom and Henry, 1991), labelling occurred preferentially in areas near pronuclear apposition. We believe that this labelling pattern reflects the concentration of DNA in this region of the pronuclei, as earlier demonstrated in cattle (Hyttel et al, 1988).…”

Section: Discussionsupporting

confidence: 85%

DNA Synthesis and pronucleus development in pig zygotes obtained in vivo: An autoradiographic and ultrastructural study

Laurinčík

Hyttel²,

Kopečný

1995

Molecular Reproduction Devel

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Porcine zygotes flushed from oviducts 48, 52, 56, 60, or 64 hr after hCG were incubated 30 min in 3H-thymidine, transferred to nonradioactive medium for 2 hr, and incubated for 30 min with 14C-thymidine. After this procedure, ova were prepared (i.e., at 51, 55, 59, 63, or 67 hr after hCG) for autoradiography and ultrastructural observations, respectively. The first autoradiographic labelling, i.e., DNA synthesis, was observed at 56-56.5 hr after hCG, while the latest labelling was seen at 60-60.5 hr. At 51 hr after hCG, formation of the pronuclear envelope was observed, while no nucleolus precursor bodies or prestages to these structures were found. At 55 hr a few clusters of small electron-dense granules were observed, together with condensed chromatin in the pronuclei. At 59 hr the apposed regions of both pronuclei contained nucleolus precursor bodies and condensed chromatin, in close contact with both clusters of small granules and clusters of an additional category of large granules and the nuclear envelope. Additionally, large accumulations of the small granules were found in the vicinity of similarly sized accumulations of the large granules without chromatin association. At 63 hr the spherical accumulations of large granules on some occasions presented a central vacuole, and condensed chromatin and clusters of small granules were attached to its periphery. Within the vacuole, electron-dense material was found.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionsupporting

confidence: 85%

DNA Synthesis and pronucleus development in pig zygotes obtained in vivo: An autoradiographic and ultrastructural study

Laurinčík

Hyttel²,

Kopečný

1995

Molecular Reproduction Devel

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“…A possible mechanism underlying the haploid set-based segregation is that each haploid compartment contains chromosomes of the same parental origin. Since the male and female pronuclei do not immediately fuse and are separated at early stages after fertilization (21, 33–39), it is plausible this parental origin may be spatially inherited as the identity for each haploid set. In support of this parental origin hypothesis, we tested two different experimental conditions: ( i ) Compartmentalization of a haploid chromosome set is spatially preserved in naturally occurring tetraploid cells (4n), and ( ii ) genetic tracing of chromosomes reveals haploid set derived by parental origin.…”

Section: Resultsmentioning

confidence: 99%

“…For example the expected values for a random distribution for 66 cells would be calculated as 66 cells/18 total 10° intervals = 3.67. A χ 2 analysis χ 2 (36) was then performed for the observed values against the expected values of 3.67 for each 10° interval and calculated for significance ( P value).…”

Section: Methodsmentioning

confidence: 99%

“…The values were calculated based on the relative frequency (number of mitotic cells measuring minimal distance at a particular interval/total mitotic cells) for a chromosome to its homologous partner or nonhomologous partner (X or Y chromosome). A Student’s t test (36) was performed for the minimum distance values between chromosome 4 and its homolog compared with chromosome 4 to a heterologous chromosome (X or Y) and calculated for significance ( P value). Note that 0–0.4 µm were grouped into a single distance interval as it is defined by the limit in confocal microscopy resolution.…”

Section: Methodsmentioning

confidence: 99%

“…A binomial probability distribution (35, 36) was employed for statistics wherein the null hypothesis is that sex and homologous chromosome pairs are randomly distributed in two hemispheres of a mitotic cell at metaphase/anaphase. For example, if distributions of chromosomes are random between two hemispheres of equal size in a cell at metaphase/anaphase, the probability of the first chromosome of a given pair’s being positioned in one hemisphere will be 1.…”

Section: Methodsmentioning

confidence: 99%

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Mitotic antipairing of homologous and sex chromosomes via spatial restriction of two haploid sets

Hua

Matsukawa

2018

Proc. Natl. Acad. Sci. U.S.A.

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SignificanceMitotic recombination must be prevented to maintain genetic stability across daughter cells, but the underlying mechanism remains elusive. We report that mammalian cells impede homologous chromosome pairing during mitosis by keeping the two haploid chromosome sets apart, positioning them to either side of a meridional plane defined by the centrosomes. Chromosome oscillation analysis revealed collective genome behavior of noninteracting chromosome sets. Male translocation mice with a maternal-derived supernumerary chromosome display the tracer chromosome exclusively to the haploid set containing the X chromosome. This haploid set-based antipairing motif is shared by multiple cell types, is doubled in tetraploid cells, and is lost in carcinoma cells. The data provide a model of nuclear polarity through the antipairing of homologous chromosomes during mitosis.

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A detailed analysis of pronucleus development in bovine zygotes in vitro: Cell-cycle chronology and ultrastructure

Laurinčík

Hyttel²,

Baran

et al. 1998

Mol. Reprod. Dev.

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The aim of the present experiment was to analyze the chronology of pronucleus development and DNA synthesis, as well as the ultrastructure of intranuclear bodies, in bovine zygotes produced in vitro. Bovine oocytes were matured and fertilized in vitro, and sperm penetration and pronucleus development were examined. DNA synthesis was investigated by sequential incubation with [3H]‐ and [14C]thymidine followed by autoradiography on semithin sections. Ultrathin sections for transmission electron microscopy were prepared from the same zygotes. Sperm penetration was noted for the first time at 4 hr after in vitro insemination and reached a maximum at 6 hr. Pronucleus formation was initiated at 4 hr, and up to at least 11 hr the maternal pronucleus was more developed than its paternal counterpart. DNA synthesis was initiated at 14–15 hr, and the S‐phase lasted for 8–10 hr. The most prominent ultrastructural entities of the pronuclei were the nucleolus precursor bodies (NPBs). During the S‐ and G2‐phases, the NPBs spatially associated with clusters of interchromatin‐like granules. The two components were firmly attached to each other by an electron‐dense reticulum. During the late G2‐phase, the NPBs were apparently detached from the interchromatin‐like granules and the electron‐dense reticulum again. The interaction between the intranuclear bodies and granules appears to be comparable with the situation previously described for in vivo‐produced bovine zygotes (J Laurinčík et al., Mol Reprod Dev 43:62–69, 1996), except for the lack of vacuolization of the NPBs during the S‐phase in vitro. Mol. Reprod. Dev. 50:192–199, 1998. © 1998 Wiley‐Liss, Inc.

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Pronuclear relationship and association of maternal and paternal chromosomes in flushed rabbit ova

Cited by 17 publications

References 16 publications

DNA Synthesis and pronucleus development in pig zygotes obtained in vivo: An autoradiographic and ultrastructural study

DNA Synthesis and pronucleus development in pig zygotes obtained in vivo: An autoradiographic and ultrastructural study

Mitotic antipairing of homologous and sex chromosomes via spatial restriction of two haploid sets

A detailed analysis of pronucleus development in bovine zygotes in vitro: Cell-cycle chronology and ultrastructure

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